Applied and Environmental Microbiology, May 2003, p . 2692-2698, Vol . 69, No . 5

Role of Listeria monocytogenes ^B in Survival of Lethal Acidic Conditions and in the Acquired Acid Tolerance Response

Adriana Ferreira,¹ David Sue,¹ Conor P . O'Byrne,² and Kathryn J . Boor^1*

Department of Food Science, Cornell University, Ithaca, New York 14853,¹ Department of Microbiology, National University of Ireland, Galway, Ireland²

Received 27 August 2002/ Accepted 3 February 2003

The adaptive ATR plays an important role in the survival of L . monocytogenes in a variety of foods (15) and in the ability of this pathogen to cause illness (26, 28) . In L . monocytogenes, acid adaptation offers cross protection against heat, ethanol, oxidative, and osmotic stresses and against the bacteriocin nisin (20, 25, 28, 36) . Adaptive ATR cross protection may also enhance the ability of L . monocytogenes to cause illness by contributing to bacterial survival of a variety of challenges imposed by a host . Such host challenges include exposure to gastric fluid, bile, and competitive intestinal flora; the presence of organic acids found in the small intestine; and the oxidative products in the phagosome (15, 37) .

One mechanism that contributes to bacterial survival under changing environmental conditions is transcription redirection through association of different alternative sigma factors with the core RNA polymerase . For example, the alternative sigma factors ^S (RpoS) (24) and ^B (21) have been associated with general stress responses in gram-negative and gram-positive bacteria, respectively . In Bacillus subtilis, ^B regulates a large general stress response regulon, contributing to the transcription of more than 100 genes that are induced by exposure to environmental stresses such as heat, acid, ethanol, salt, and oxidative stress (1, 2, 14, 31, 33, 38) . In Staphylococcus aureus, ^B activity is induced by mild acid stress (7) and the adaptive ATR in S . aureus appears to be ^B mediated (9) . In L . monocytogenes, ^B contributes to cellular survival under several adverse conditions, such as carbon depletion and exposure to acid, oxidative, low temperature, and osmolarity stresses (5, 6, 16) . We have found previously that L . monocytogenes cells in the stationary phase display two distinct mechanisms of AR: a ^B-dependent AR mechanism and an ATR mechanism that is at least partially ^B independent (16) . In this study, we investigated the contributions of ^B to acid tolerance throughout growth in L . monocytogenes . To define a possible role for ^B in additional L . monocytogenes AR mechanisms, we also examined the effects of loss of ^B function on intracellular pH buffering and in net charge flux across the cell membrane at pH 2.5 .

Construction of gus fusion strains.
The plasmid pNF580 (19), which bears the gus gene, was used to construct L . monocytogenes strains containing chromosomal gus reporter fusions with the opuCA promoter region . Specifically, a 312-bp fragment bearing the predicted opuCA promoter region (17) was amplified by using primers opuCA-F1 (5' GCG GAT CCC GTG CCA CAA GTA CGA AAT CA 3') and opuCA-R1 (5' GCT CTA GAA TCA TCT TCA TTG TTG TCG TT 3') . PCR was performed by using Vent polymerase (New England Biolabs, Beverly, Mass.) and the following cycling conditions: 3 min at 94°C for 1 cycle; 30 s at 94°C, 30 s at 58°C, and 30 s at 72°C for 35 cycles; and a final extension of 5 min at 72°C .

PCR products were purified by using the QiaQuick PCR purification kit (Qiagen, Valencia, Calif.) and digested by using XbaI and BamHI for cloning upstream of the promoterless gus gene in pNF580 . The resulting pDS2 plasmid, which bears an opuCA-gus fusion, was constructed in OneShot Escherichia coli cells (Invitrogen, Carlsbad, Calif.) . The plasmid was electroporated into both L . monocytogenes 10403S and L . monocytogenes FSL A1-254 . Transformants were selected on brain heart infusion (BHI; Difco, Sparks, Md.) agar plates containing 10 µg of chloramphenicol/ml . Isolates were serially passaged at 42°C in BHI broth containing 10 µg of chloramphenicol/ml to allow for selection of chromosomal integration of the fusion plasmids . Chromosomal integration of the plasmids was confirmed by PCR and sequence analysis . The sigB genotype in each strain was verified by PCR .

Growth conditions and cell viability.
Single colonies from BHI agar plates were used to inoculate tubes containing 5 ml of BHI broth, which were then incubated overnight . All cultures were grown in BHI and incubated with shaking (250 rpm) at 37°C . The overnight cultures were then subcultured (1:1,000) in 10 ml of BHI and grown to an optical density at 600 nm (OD₆₀₀) of 0.4 prior to all experiments, except where otherwise indicated . For acid stress assays, a 6 N HCl solution was used to acidify the media to pH 4.5 or 2.5 . For all experiments, cell viabilities were assessed by serially diluting 100-µl culture aliquots in 900 µl of phosphate-buffered saline, plating 100 µl of each dilution on BHI agar, incubating them for approximately 18 h at 37°C, and enumerating the cells by standard plate counting procedures .

Acid tolerance during L . monocytogenes growth.
Cell viabilities were compared after exposure to BHI, pH 2.5, with and without preexposure to a milder acidic environment (BHI, pH 4.5), as previously described (16) but with the following modifications . Cell cultures that had been grown to an OD₆₀₀ of 0.4 as described above were subcultured again (1:200) in 100 ml of fresh BHI . At several time points throughout growth, 1-ml samples were collected and cells were harvested by centrifugation at 13,000 x g for 2 min . The cell pellets were resuspended in 1 ml of BHI (pH 2.5) and incubated at 37°C for 60 min . For adaptation, cell pellets were resuspended in 1 ml of BHI (pH 4.5) and incubated for 60 min before centrifugation and resuspension in BHI (pH 2.5) . Cell viability was measured at 0 and 60 min after lethal acid (pH 2.5) exposure . Results are reported as the means of data collected from two independent replications .

Survival in synthetic gastric fluid.
Cell cultures at an OD₆₀₀ of 0.4 were subcultured (1:200) in 100 ml of fresh BHI, and the cells were harvested at the early log (OD₆₀₀ = 0.2), mid-log (OD₆₀₀ = 0.4), and stationary (16 h after medium inoculation) phases . Cells were resuspended in 1 ml of synthetic gastric fluid (8.3 g of proteose peptone liter^-1, 3.5 g of D-glucose liter^-1, 2.05 g of NaCl liter^-1, 0.6 g of KH₂PO₄ liter^-1, 0.11 g of CaCl₂ liter^-1, 0.37 g of KCl liter^-1, 0.05 g of bile liter^-1, 0.1 g of lysozyme liter^-1, and 13.3 mg of pepsin liter^-1; adjusted to pH 2.5 with 6 N HCl) (10) and incubated at 37°C for 60 min . For adaptation, cells were resuspended in 1 ml of BHI (pH 4.5) and incubated for 60 min before resuspension in synthetic gastric fluid . Cell viability was measured as described above at 0 and 60 min after exposure to synthetic gastric fluid . Results are reported as the means of three independent experiments, each performed in duplicate .

Survival in organic acid.
Cell survival in the presence of acetic acid was compared with cell survival in the presence of inorganic acid (HCl) at pH 2.5 . Aliquots (1 ml) of overnight cell cultures were centrifuged at 13,000 x g for 2 min . Harvested cells were resuspended in 1 ml of BHI (pH 2.5) (inorganic acid), 1 ml of BHI (pH 2.5) (10 mM acetic acid), or 1 ml of BHI (pH 2.5) (100 mM acetic acid) and incubated for 60 min at 37°C . Cell viability was measured as described above at 0 and 60 min after exposure to the acidified BHI media . Results are reported as the means of three independent experiments performed in duplicate . The t test was used to calculate P values for these assay results .

GUS activity assay.
GUS activity assays were performed on L . monocytogenes strains FSL S1-059 (sigB, opuCA-gus fusion) and FSL S1-063 (wild-type ^B, opuCA-gus fusion) collected at several time points during growth . Cell cultures at an OD₆₀₀ of 0.4 (grown from overnight cultures as described previously) of either L . monocytogenes FSL S1-059 or FSL S1-063 were used to subculture (1:200) 100 ml of BHI media . Aliquots (1 ml) were harvested at several points throughout growth by centrifugation at 13,000 x g for 2 min and were washed and resuspended in 400 µl of AB light buffer (0.1 M potassium phosphate [pH 7.0], 60 mM K₂HPO₄, 40 mM KH₂PO₄, 0.1 M NaCl) . An aliquot of 100 µl was removed and serially diluted in phosphate-buffered saline to determine cell numbers . The remaining 300 µl was frozen in liquid nitrogen and stored at -80°C .

For the GUS activity assay, 50 µl of AB light buffer with 0.1% Triton X-100 was added to 50 µl of thawed cell suspensions, and the mixtures were incubated at room temperature for 60 min . The mixtures were incubated again for 60 min at room temperature following the addition of 20 µl of the GUS substrate, 4-methylumbelliferyl ß-D-glucoronide (4-MUG) (Sigma-Aldrich Corp., St . Louis, Mo.) . 4-MUG is hydrolyzed by GUS into the fluorescent molecule methylumbelliferone (MU^-) . Relative fluorescence units were measured in a Fusion 96-well plate reader (Packard Instruments, Boston, Mass.) with excitation and emission wavelengths set at 365 and 455 nm, respectively . GUS activity was expressed as picomoles of 4-MUG cleaved per minute per log CFU . Results are reported as the means of two independent experiments, each performed in duplicate .

Net proton flux assay.
The net proton flux assay was performed as described by Jordan et al . (22) with the following modifications . Cells from overnight cultures were harvested, washed, and resuspended in 100 mM KCl at a concentration of 30 mg (wet weight) of cells/ml . The pH of a 20-ml cell suspension in 100 mM KCl was adjusted with 0.5 M HCl to pH 2.5 . The solution was stirred continuously . Following a 1-min equilibration period, the pH was recorded at 30-s intervals for 10 min . An increase in the medium pH was assumed to represent a net flux of protons across the cell membrane . The pH is calculated as the external pH (pH_o) at a given time minus the initial pH_o . Results are reported as the means of three independent experiments performed in duplicate .

GAD assay.
The glutamate decarboxylase (GAD) assay was used to measure the extracellular concentration of gamma aminobutyric acid (GABA) present in the medium under assay conditions . This assay was performed as described by Cotter et al . (10) with the following modifications . Stationary-phase cells were harvested and resuspended in BHI, pH 2.5 . After a 60-min incubation at 37°C, 20 µl of spent cell culture medium was incubated for 30 min at 37°C with 80 µl of the assay solution (0.3 M Tris buffer [pH 8.9], 10 mM -ketoglutarate, 2 mM 2-mercaptoethanol, 0.5 mM NADPH, and 1 U of GABase/ml; Sigma-Aldrich Corp.) . One unit of GAD was defined as that which catalyzes the formation of 1 µmol of GABA min^-1 mg of total protein^-1 . The amount of GABA in the spent medium was deduced from the amount of NADPH produced in the assay . The NADPH formed was measured spectrophotometrically at 340 nm . Total protein was calculated using the Bio-Rad (Hercules, Calif.) protein assay . Results are reported as the means of three independent experiments performed in duplicate . The t test was used to calculate P values for these assay results .

 
As shown in Fig . 1, preexposure to mild acid (pH 4.5) also enhanced the survival of both wild-type and sigB cells to subsequent exposure to lethal acid (pH 2.5) . Wild-type strain survival increased by 5 orders of magnitude in early log-phase cells and by 4 orders of magnitude in mid-log phase cells but was not enhanced in stationary-phase cells (Fig . 1) . Preexposed sigB cell survival increased by 2 and 3 orders of magnitude in the early and mid-log phases but also by 1 order of magnitude in the stationary phase . Therefore, while ^B contributes to adaptive ATR, with the greatest ^B-specific effects observed in exponential-phase cells, L . monocytogenes also appears to have a pH-dependent, ^B-independent acid tolerance mechanism that can be induced throughout growth .

^B activity is induced by entry into stationary phase.
^B activity was deduced from GUS activity measured in strains FSL S1-059 (sigB) and FSL S1-063 (wild-type ^B), each of which bears a transcriptional gus fusion to the ^B-dependent opuCA promoter . The GUS activity assay was determined by using cells collected throughout growth . As shown in Fig . 2, during early log growth and up to 5 h postinoculation, GUS activity in FSL S1-063 was low (<10 GUS activity units) . However, FSL S1-063 GUS activity increased to >30 GUS activity units upon entry into and throughout the stationary phase, whereas, in the absence of an intact sigB, GUS activity in FSL S1-059 never exceeded background levels (<3 GUS activity units) throughout growth .

 
^B enhances cell survival in synthetic gastric fluid.
To determine if the contribution of ^B to acid survival in a rich medium also could be extended to conditions mimicking passage though the human stomach, we compared the relative survival of the sigB and the wild-type strains in synthetic gastric fluid . Wild-type and sigB cultures at early log, mid-log, and stationary phase were assayed for survival in gastric fluid with and without preexposure to pH 4.5 . As shown in Fig . 3, entry into the stationary phase enhanced the survival of non-acid-adapted cells for both strains in synthetic gastric fluid, suggesting that, as with survival to lethal acid exposure, the ability of L . monocytogenes to survive in gastric fluid is growth phase dependent . Survival in synthetic gastric fluid was at least partially ^B dependent, as survival of stationary-phase sigB cells was over 4 orders of magnitude lower than that observed for the wild-type strain .

 
In addition to resistance to synthetic gastric fluid throughout growth, ^B also appears to contribute to the acquisition of enhanced resistance to synthetic gastric fluid following preexposure to milder acidic conditions in log-phase L . monocytogenes cells . Specifically, during early log phase, the percent survival of the wild-type strain that had been preexposed to pH 4.5 increased by almost 3 orders of magnitude . Recoveries of both the nonadapted sigB cells and the preexposed sigB cells remained below detection limits (10 CFU/ml) for early log-phase cells . Mid-log preexposed wild-type cells had a larger increase in percent survival (5 orders of magnitude) than mid-log sigB cells (3 orders of magnitude) . No additional increment in AR was achieved in preexposed wild-type stationary-phase cells, as nonexposed stationary-phase wild-type cells already displayed approximately 100% recovery after 60 min in gastric fluid . On the other hand, the percent survival of stationary-phase sigB cells that had been preexposed to pH 4.5 increased over 4 orders of magnitude, suggesting the presence of a ^B-independent pH-dependent mechanism for enhanced tolerance to stresses imposed by synthetic gastric fluid in stationary-phase L . monocytogenes cells .

Role of ^B in organic acid stress.
We also investigated the contribution of ^B to protection of L . monocytogenes from organic acid stress relative to protection from inorganic acid stress . Specifically, we compared the relative abilities of stationary-phase cells of the wild-type and sigB strains to survive stress associated with undissociated acetic acid or with HCl alone at an external pH of 2.5 .

As shown in Fig . 4, wild-type strain survival did not differ following exposure to organic acid (either 10 or 100 mM acetic acid; P = 0.41 or 0.043, respectively) or inorganic acid alone . Specifically, wild-type survival was reduced by 70% in 100 mM acetic acid and by 50% in HCl alone under our experimental conditions . On the other hand, sigB strain survival was more than 1 log lower in the presence of 100 mM acetic acid (P = 0.0074) than in the presence of either 10 mM acetic acid (P = 0.044) or inorganic acid at the same pH . These results suggest that survival of L . monocytogenes in organic acid under these conditions is enhanced by the presence of ^B . ^B appears to contribute to L . monocytogenes survival both under low external pH conditions and in the presence of undissociated acetic acid .

 
Role of ^B in net proton flux.
To investigate possible ^B-directed mechanisms contributing to AR in L . monocytogenes, we compared net proton fluxes for wild-type and sigB cells exposed to pH 2.5 . Net proton flux was inferred by the pH in the cell suspension (pH_o) observed during a 10-min period following the adjustment of the suspension pH to 2.5 with HCl . No difference was observed in the pH between the sigB and the wild-type strains during these 10-min periods (data not shown) . An increase of approximately 0.40 U in pH_o was observed for both strains . Neither strain demonstrated viability loss during the 10-min assay, as determined by viable plate counts . These results suggest that net proton movement across cell membranes is not dependent on ^B under these conditions .

^B regulation of glutamate decarboxylase.
Buffering mechanisms for maintenance of a near-neutral pH (pH_i) can contribute to the survival of neutralophilic bacteria following exposure to low-pH environments (3, 8) . Therefore, we also investigated whether ^B contributes to pH_i buffering in L . monocytogenes through regulation of the GAD system . This system has been described previously for E . coli (35) and Shigella flexneri (39) and has only recently been characterized in L . monocytogenes (10) . The GAD system encompasses at least 2 proteins: a GABA antiporter at the cell membrane and a cytoplasmic glutamate decarboxylase enzyme . When exposed to low pH, the GAD system is believed to offer protection from reduced pH by consuming an intracellular proton to convert an extracellular glutamate (i.e., a glutamate from the medium that is transported into the cell) into GABA, which is then transported out of the cell . Thus, this reaction may decrease the net proton concentration in the cytoplasm . To determine if the GAD system is under ^B regulation in L . monocytogenes, we compared GAD activity in the wild-type strain with that of the sigB strain after exposing cells to pH 2.5 for 60 min . GAD activities of the wild-type (273 ± 46) and the sigB (338 ± 49) strains did not differ (P = 0.35, t test) under the conditions used in this study . These results suggest that GAD activity is not ^B dependent under these conditions .

The relative contributions of ^B to the adaptive ATR appear to be greater in log-phase L . monocytogenes cells than in stationary-phase cells . The regulatory function of ^B in log-phase adaptive ATR is likely to be due to rapid induction of ^B activity and, hence, transcription of ^B-regulated genes following acid exposure, since the level of ^B activity during log phase and particularly during early log phase is very low under balanced growth conditions (Fig . 2) . Becker et al . (5) inferred from primer extension analyses that ^B activity in log-phase L . monocytogenes can be induced from nondetectable levels to a level similar to that observed for stationary-phase cells following exposure of cells to mildly acidified media (pH 5.3) . Similarly, ^B activity is induced by mild acid exposure in log-phase B . subtilis (23) and S . aureus (7), and acid-induced log-phase ATR in B . subtilis and S . aureus is at least partially ^B dependent (9, 38) . It is possible that the ^B-independent mechanism that appears to be growth phase dependent, and that appears to contribute to protection against acid stress in late-log- and stationary-phase L . monocytogenes cells, cannot be induced during the early phases of growth .

Adaptive ATR can also confer cross protection against other stress conditions . Acid-adapted L . monocytogenes shows enhanced resistance against heat, ethanol, osmotic stress, or stress due to crystal violet, a surface-active agent (28), while heat adaptation results in enhanced protection against acid stress (32) . At least 37 proteins are induced by mild acid (pH 5.5) exposure in L . monocytogenes, while over 47 proteins are induced by exposure to pH 3.5 . Between these groups of proteins, 23 overlap (32) . These 23 proteins are likely to contribute to enhanced cellular survival in lethal acid conditions following exposure to mild acid conditions

L . monocytogenes resistance to organic acid exposure.
Protonated organic acids cross cell membranes more freely than inorganic acid molecules (11, 13, 30, 41) . Under the conditions used in these experiments (pH 2.5) the majority of acetic acid molecules (99.5%) are protonated . Once inside the higher pH environment of the cell, protonated molecules dissociate, which causes the pH_i of the cell to decrease (4) . Accordingly, the pH_i of L . monocytogenes cells exposed to protonated molecules of acetic acid is lower than that of cells exposed to HCl at a similar external pH (41) . However, as equivalent concentrations of different organic acids have different inhibitory effects on L . monocytogenes (41), a reduction of the internal pH is unlikely to be the sole stress associated with the presence of organic acids . It has been proposed that the presence of organic acids inhibits bacterial cell growth by decreasing cellular pH_i, by increasing energy consumption for pH homeostasis, and by disrupting substrate transport and macromolecular synthesis (8) .

Loss of a functional ^B reduced survival of L . monocytogenes to a greater extent at pH 2.5 in the presence of organic acid (100 mM of acetic acid) than in the presence of inorganic acid (HCl alone) . O'Driscoll et al . (29) demonstrated that different protein expression patterns are observed in L . monocytogenes cells exposed to organic acids than in cells exposed to inorganic acids . In combination with our findings, these observations suggest that the physiological requirements for the survival of organic and inorganic acid challenges differ and that ^B may mediate L . monocytogenes protection against organic and inorganic acid stresses through different mechanisms .

^B-directed mechanisms of AR.
We investigated the role of ^B in pH_i homeostasis through regulation of net proton movement across the cell membrane or by regulation of pH_i buffering by the GAD system . Recently, Shabala et al . (34) investigated proton flux in L . monocytogenes at several pH levels in the presence or absence of glucose . Cells were shown to initiate net proton efflux after 5 min at pH 4 or 6 in the presence of 10 mM glucose while under similar pH conditions, but in the absence of glucose, a continuous net proton influx was observed for a period of at least 50 min . As our experiment was conducted in the absence of glucose, the observed +0.4 pH_o increment in the medium following exposure of cells to pH 2.5 may be due to a net proton influx . Although a loss of viability was not detected for either strain during the time course of this assay, it is also possible that the pH_o is due, in part, to cellular leakage of alkaline materials resulting from membrane damage caused by the extremely low pH . Under the conditions used in these studies, net proton movement across cell membranes and regulation of the GAD system for pH_i buffering both appear to be ^B independent in L . monocytogenes . However, it is possible that ^B contributes to pH_i buffering in L . monocytogenes through regulation of an as yet unknown system .

The results from this study contribute to our understanding of AR mechanisms in L . monocytogenes. We have shown that ^B contributes to cellular survival through at least two mechanisms in L . monocytogenes: (i) a general acid tolerance to which ^B contributes throughout all growth phases and (ii) a pH-inducible ATR mechanism that is at least partially ^B dependent in exponential-phase cells . Furthermore, our results suggest that ^B may contribute to the success of L . monocytogenes as a pathogen by enhancing its survival during passage through the gastric fluid .

1. Antelmann, H., S . Engelmann, R . Schmid, and M . Hecker. 1996 . General and oxidative stress responses in Bacillus subtilis: cloning, expression, and mutation of the alkyl hydroperoxide reductase operon . J . Bacteriol . 178:6571-6578.
2. Antelmann, H., S . Engelmann, R . Schmid, A . Sorokin, A . Lapidus, and M . Hecker. 1997 . Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor ^B in Bacillus subtilis . J . Bacteriol . 179:7251-7256.
3. Audia, J . P., C . C . Webb, and J . W . Foster. 2001 . Breaking through the acid barrier: an orchestrated response to proton stress by enteric bacteria . Int . J . Med . Microbiol . 291:97-106.
4. Bearson, S., B . Bearson, and J . W . Foster. 1997 . Acid stress responses in enterobacteria . FEMS Microbiol . Lett . 147:173-180.
5. Becker, L . A., M . S . Cetin, R . W . Hutkins, and A . K . Benson. 1998 . Identification of the gene encoding the alternative sigma factor ^B from Listeria monocytogenes and its role in osmotolerance . J . Bacteriol . 180:4547-4554.
6. Becker, L . A., S . N . Evans, R . W . Hutkins, and A . K . Benson. 2000 . Role of ^B in adaptation of Listeria monocytogenes to growth at low temperature . J . Bacteriol . 182:7083-7087.
7. Bischoff, M., J . M . Entenza, and P . Giachino. 2001 . Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus . J . Bacteriol . 183:5171-5179.
8. Booth, I . R. 1985 . Regulation of cytoplasmic pH in bacteria . Microbiol . Rev . 49:359-378.
9. Chan, P . F., S . J . Foster, E . Ingham, and M . O . Clements. 1998 . The Staphylococcus aureus alternative sigma factor ^B controls the environmental stress response but not starvation survival or pathogenicity in a mouse abscess model . J . Bacteriol . 180:6082-6089.
10. Cotter, P . D., C . G . Gahan, and C . Hill. 2001 . A glutamate decarboxylase system protects Listeria monocytogenes in gastric fluid . Mol . Microbiol . 40:465-475.
11. Cramer, J . A., and J . H . Prestegard. 1977 . NMR studies of pH-induced transport of carboxylic acids across phospholipid vesicle membranes . Biochem . Biophys . Res . Commun . 75:295-301.
12. Davis, M . J., P . J . Coote, and C . P . O'Byrne. 1996 . Acid tolerance in Listeria monocytogenes: the adaptive acid tolerance response (ATR) and growth-phase-dependent acid resistance . Microbiology 142:2975-2982.
13. Eklund, T. 1983 . The antimicrobial effect of dissociated and undissociated sorbic acid at different pH levels . J . Appl . Bacteriol . 54:383-389.
14. Engelmann, S., C . Lindner, and M . Hecker. 1995 . Cloning, nucleotide sequence, and regulation of katE encoding a sigma B-dependent catalase in Bacillus subtilis . J . Bacteriol . 177:5598-5605.
15. Farber, J . M., and P . I . Peterkin. 1991 . Listeria monocytogenes, a food-borne pathogen . Microbiol . Rev . 55:476-511.
16. Ferreira, A., C . P . O'Byrne, and K . J . Boor. 2001 . Role of ^B in heat, ethanol, acid, and oxidative stress resistance and during carbon starvation in Listeria monocytogenes . Appl . Environ . Microbiol . 67:4454-4457.
17. Fraser, K . R., D . Harvie, P . J . Coote, and C . P . O'Byrne. 2000 . Identification and characterization of an ATP binding cassette L-carnitine transporter in Listeria monocytogenes . Appl . Environ . Microbiol . 66:4696-4704.
18. Fraser, K . R., D . Sue, M . Wiedmann, K . Boor, and C . P . O'Byrne. 2003 . Role of ^B in regulating the compatible solute uptake systems of Listeria monocytogenes: osmotic induction of opuC is ^B dependent . Appl . Environ . Microbiol . 69:2015-2022.
19. Freitag, N . E. 2000 . Genetic tools for use with Listeria monocytogenes, p . 488-498 . In V . A . Fischetti (ed.), Gram-positive pathogens . American Society for Microbiology, Washington D.C.
20. Gahan, C . G., B . O'Driscoll, and C . Hill. 1996 . Acid adaptation of Listeria monocytogenes can enhance survival in acidic foods and during milk fermentation . Appl . Environ . Microbiol . 62:3128-3132.
21. Haldenwang, W . G., and R . Losick. 1980 . Novel RNA polymerase sigma factor from Bacillus subtilis . Proc . Natl . Acad . Sci . USA 77:7000-7004.
22. Jordan, K . N., L . Oxford, and C . P . O'Byrne. 1999 . Survival of low-pH stress by Escherichia coli O157:H7: correlation between alterations in the cell envelope and increased acid tolerance . Appl . Environ . Microbiol . 65:3048-3055.
23. Kovacs, T., A . Hargitai, K . L . Kovacs, and I . Mecs. 1998 . pH-dependent activation of the alternative transcriptional factor ^B in Bacillus subtilis . FEMS Microbiol . Lett . 165:323-328.
24. Loewen, P . C., and R . Hengge-Aronis. 1994 . The role of the sigma factor sigma S (KatF) in bacterial global regulation . Annu . Rev . Microbiol . 48:53-80.
25. Lou, Y., and A . E . Yousef. 1997 . Adaptation to sublethal environmental stresses protects Listeria monocytogenes against lethal preservation factors . Appl . Environ . Microbiol . 63:1252-1255.
26. Marron, L., N . Emerson, C . G . Gahan, and C . Hill. 1997 . A mutant of Listeria monocytogenes LO28 unable to induce an acid tolerance response displays diminished virulence in a murine model . Appl . Environ . Microbiol . 63:4945-4947.
27. Mead, P . S., L . Slutsker, V . Dietz, L . F . McCaig, J . S . Bresee, C . Shapiro, P . M . Griffin, and R . V . Tauxe. 1999 . Food-related illness and death in the United States . Emerg . Infect . Dis . 5:607-625.
28. O'Driscoll, B., C . G . Gahan, and C . Hill. 1996 . Adaptive acid tolerance response in Listeria monocytogenes: isolation of an acid-tolerant mutant which demonstrates increased virulence . Appl . Environ . Microbiol . 62:1693-1698.
29. O'Driscoll, B., C . G . M . Gahan, and C . Hill. 1997 . Two-dimensional polyacrylamide gel electrophoresis analysis of the acid tolerance response in Listeria monocytogenes LO28 . Appl . Environ . Microbiol . 63:2679-2685.
30. Ostling, C . E., and S . E . Lindgren. 1993 . Inhibition of enterobacteria and Listeria growth by lactic, acetic and formic acids . J . Appl . Bacteriol . 75:18-24.
31. Petersohn, A., M . Brigulla, S . Haas, J . D . Hoheisel, U . Volker, and M . Hecker. 2001 . Global analysis of the general stress response of Bacillus subtilis . J . Bacteriol . 183:5617-5631.
32. Phan-Thanh, L., F . Mahouin, and S . Alige. 2000 . Acid responses of Listeria monocytogenes . Int . J . Food Microbiol . 55:121-126.
33. Price, C . W., P . Fawcett, H . Ceremonie, N . Su, C . K . Murphy, and P . Youngman. 2001 . Genome-wide analysis of the general stress response in Bacillus subtilis . Mol . Microbiol . 41:757-774.
34. Shabala, L., B . Budde, T . Ross, H . Siegumfeldt, M . Jakobsen, and T . McMeekin. 2002 . Responses of Listeria monocytogenes to acid stress and glucose availability revealed by a novel combination of fluorescence microscopy and microelectrode ion-selective techniques . Appl . Environ . Microbiol . 68:1794-1802.
35. Smith, D . K., T . Kassam, B . Singh, and J . F . Elliott. 1992 . Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci . J . Bacteriol . 174:5820-5826.
36. van Schaik, W., C . G . Gahan, and C . Hill. 1999 . Acid-adapted Listeria monocytogenes displays enhanced tolerance against the lantibiotics nisin and lacticin 3147 . J . Food Prot . 62:536-539.
37. Vazquez-Boland, J . A., M . Kuhn, P . Berche, T . Chakraborty, G . Dominguez-Bernal, W . Goebel, B . Gonzalez-Zorn, J . Wehland, and J . Kreft. 2001 . Listeria pathogenesis and molecular virulence determinants . Clin . Microbiol . Rev . 14:584-640.
38. Volker, U., B . Maul, and M . Hecker. 1999 . Expression of the ^B-dependent general stress regulon confers multiple stress resistance in Bacillus subtilis . J . Bacteriol . 181:3942-3948.
39. Waterman, S . R., and P . L . Small. 1996 . Identification of sigma S-dependent genes associated with the stationary-phase acid-resistance phenotype of Shigella flexneri . Mol . Microbiol . 21:925-940.
40. Wiedmann, M., T . J . Arvik, R . J . Hurley, and K . J . Boor. 1998 . General stress transcription factor ^B and its role in acid tolerance and virulence of Listeria monocytogenes . J . Bacteriol . 180:3650-3656.
41. Young, K . M., and P . M . Foegeding. 1993 . Acetic, lactic and citric acids and pH inhibition of Listeria monocytogenes Scott A and the effect on intracellular pH . J . Appl . Bacteriol 74:515-520.

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