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Binding of  A and
B to Core RNA Polymerase after Environmental Stress in Bacillus subtilis.Claudia Rollenhagen, 2003.In Bacillus subtilis, the alternative sigma factor B is activated in response to environmental stress or energy depletion . The general stress regulon under the control of
B provides the cell with multiple stress resistance . Experiments were designed to determine how activated
B replaces
A as a constituent of the RNA polymerase holoenzyme . Studies of the transcription of the
A-dependent stress gene clpE under
B-inducing conditions showed that expression was higher in a sigB mutant background than in the wild type . The relative affinities of
A and
B for binding to the core RNA polymerase (E) were determined by means of indirect surface plasmon resonance . The results showed that the affinity of
B for E was 60-fold lower than that of
A . Western blot analyses with antibodies against
A,
B, and E showed that, after exposure to ethanol stress, the concentration of
B was only twofold higher than those of
A and E . Thus, the concentration of
B after stress is not high enough to compensate for its relatively low affinity for E, and it seems that additional mechanisms must be invoked to account for the binding of
B to E after stress .
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