Journal of Bacteriology, January 2003, p . 20-27, Vol . 185, No . 1

Cinnamate:Coenzyme A Ligase from the Filamentous Bacterium Streptomyces coelicolor A3(2)

Masafumi Kaneko, Yasuo Ohnishi, and Sueharu Horinouchi^*

Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan

Received 29 July 2002/ Accepted 9 October 2002

4CL-catalyzed CoA ester formation takes place via a two-step reaction . During the first step, 4-coumarate and ATP form a coumaroyl-adenylate intermediate with the simultaneous release of pyrophosphate . In the second step, the coumaroyl group is transferred to the sulfhydryl group of CoA, and AMP is released (2, 19) . The mechanism of formation of an adenylate intermediate is common among a number of enzymes with divergent functions, including luciferases, fatty acyl-CoA ligases, acetyl-CoA ligases, and specialized domains within peptide synthetase multienzymes . Despite their low overall amino acid sequence identity, similar reaction mechanisms and the presence of conserved peptide motifs were used as criteria to classify them in a superfamily of adenylate-forming enzymes (13) . The relationship of 4CL to other adenylate-forming enzymes was substantiated recently by functional analysis of key 4CL amino acid residues that are conserved in other adenylate-forming enzymes (25) . Phylogenetic analyses within the superfamily of adenylate-forming enzymes show that 4CL forms a monophyletic plant-specific group that is most closely related to luciferases rather than long-chain acyl-CoA ligases and acetyl-CoA ligases (7) .

The filamentous, soil-living, gram-positive bacterial genus Streptomyces is characterized by the ability to produce a wide variety of secondary metabolites, including antibiotics, and by complex morphological differentiation culminating in sporulation (17) . Streptomyces coelicolor A3(2) is the best genetically characterized strain within the Streptomyces genus, and recently the whole genome has been sequenced (http://www.sanger.ac.uk/Projects/S_coelicolor) . In the database, a gene (SCD10.15) encoding a 522-amino-acid protein has been annotated as a 4CL gene . The protein shows higher similarity in amino acid sequence to plant 4CLs than to bacterial acyl-CoA ligases; it shows 44% identity and 58% similarity to Arabidopsis thaliana 4CL2 . This is the first bacterial protein which shows end-to-end sequence similarity to plant 4CLs over 40% identity .

The purpose of this study was to reveal the enzymatic properties of the 4CL homologue . We cloned and expressed the SCD10.15 gene in Escherichia coli . The recombinant protein showed distinct 4CL activity, but its substrate specificity turned out to be unique; the enzyme efficiently converted cinnamate, which is a very poor substrate for plant 4CL . The enzyme was therefore named ScCCL for S . coelicolor A3(2) cinnamate:CoA ligase . Site-directed mutagenesis of ScCCL was also carried out to elucidate the amino acid residues providing the unique substrate specificity of ScCCL .

Plasmid construction. On the basis of the nucleotide sequence of SCD10.15 in the DNA database, the ScCCL gene (AL359988) was amplified by PCR with S . coelicolor A3(2) chromosomal DNA as a template and the following two primers: N-Hind-Nde (5'-GCGaagcttCATATGTTCCGCAGCGAGTACGCAGAC-3'; the italic letters indicate the start codon of the ScCCL gene, and the lowercase letters and underlined letters indicate HindIII and NdeI sites, respectively) and C-Bam (5'-CGCGGATCCTCATCGCGGCTCCCTGAGCTGTC-3'; the italic letters indicate the termination codon of the ScCCL gene, and the underlined letters indicate a BamHI site) . The amplified fragment was digested with HindIII plus BamHI and cloned into pUC19 . The absence of undesired alterations was checked by nucleotide sequencing . The ScCCL gene was then excised with NdeI and BamHI and cloned into pET16b, generating pET-ScCCL . The expression plasmid pET-ScCCL directs the synthesis of a protein (calculated M_r, 58,000) with the structure Met-Gly-His₁₀-Ser₂-Gly-His-Ile-Glu-Gly-Arg-His-ScCCL .

Generation of mutant ScCCL. The ScCCL sequence in pUC19-ScCCL was divided into three fragments, and restriction sites were introduced by PCR amplification with the following primers: fragment I-F, 5'-GCGaagcttCATATGTTCCGCAGCGAGTACGCAGAC-3' (the lowercase letters and underlined letters indicate HindIII and NdeI sites, respectively); fragment I-R, 5'-ACGGCATGCTCGGTTCGAGCTGGGCGAGGT-3' (the underlined letters indicate an SphI site); fragment II-F, 5'-CGTGCATGCCGTCCGCGCCCGGCGA-3' (the underlined letters indicate an SphI site); fragment II-R, 5'-CTCCTGCAGGGAGGTCCGTGCCCGGGTC-3' (the underlined letters indicate a PstI site); fragment III-F, 5'-TCCCTGCAGGAGAGTCCGGGGAGATCCT-3' (the underlined letters indicate a PstI site); and fragment III-R, 5'-CGCGGATCCTCATCGCGGCTCCCTGAGCTGTC-3' (the underlined letters indicate a BamHI site) . The resulting three fragments were cloned into pUC19 .

Fragment III was excised and ligated into pUC19-fragment I to generate pUC19-fragment I-fragment III . Fragment II was mutagenized with the QuikChange multisite-directed mutagenesis kit (Stratagene) with the following primers: T228N, 5'-ATCTACGGCCTGAACGCCCTGATGAACGCCCCG-3' (the altered nucleotides are shown in italics); Y265A, 5'-CGCATCACCAGCCTGGCCGTCGCCCCGCCG-3'; A294G, 5'-TACATCGTCAGCGGCGCCGCCCCGCTCGACGCG-3'; A318G, 5'-CCGCCCGTCGGCCAGGGCTACGGCATGACC-3'; and G327V, 5'-GAACTGTCCCCGGTCACCCACGTCGTCCCC-3' . Mutagenized fragment II was excised and ligated between fragment I and fragment III in pUC19-fragment I-fragment III . The mutated ScCCL sequences were recombined with pET16b and expressed as described below .

Expression and purification of histidine-tagged ScCCL. We examined the culture conditions of E . coli BL21(DE3) harboring pET16b-ScCCL to produce the histidine-tagged ScCCL in a soluble form as much as possible, because the protein was found in both soluble and insoluble fractions under the usual culture conditions . After cultivation temperature, inoculum size, and induction of the T7 promoter via the lac operator with isopropyl-ß-D-thiogalactopyranoside (IPTG) had been examined, we established the following cultivation conditions . E . coli BL21(DE3) harboring pET-ScCCL was cultured at 37°C for 8 h in Luria-Bertani broth (1% Bacto-tryptone, 0.5% Bacto yeast extract, and 1% NaCl) containing 50 µg of ampicillin per ml, and 60 µl of the seed culture was transferred to 30 ml of the same medium containing 100 µg of ampicillin per ml and cultured at 26.5°C for 18 h without induction with IPTG . The cells were harvested from 30 ml of culture by centrifugation and suspended in 600 µl of buffer A (40 mM NaH₂PO₄, 240 mM NaCl, 8 mM imidazole, and 20% ethylene glycol, pH 8.0) . After the cells had been disrupted by sonication, a soluble fraction obtained by centrifugation at 20,000 x g of the sonicate was applied to the Ni-nitrilotriacetic acid spin column (Qiagen), and the histidine-tagged ScCCL was eluted according to the manual of the supplier . Purification was monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis .

Enzyme assays. 4CL activity was determined by the spectrophotometric assay, as previously described (20), with various cinnamate derivatives as phenolic substrates . Cinnamate and its derivatives were purchased from Sigma-Aldrich . CoA was purchased from Oriental Yeast . The changes in absorbance during CoA ester formation were monitored at wavelengths of 333, 311, 369, 345, and 352 nm, depending on the absorption maxima for 4-coumaroyl-CoA, cinnamoyl-CoA, caffeoyl-CoA, feruloyl-CoA, and sinapoyl-CoA, respectively (24) . The standard reaction mix, in a total volume of 1 ml, contained 400 mM Tris-HCl (pH 7.8), 5 mM ATP, 5 mM MgCl₂, 0.3 mM CoA, 0.5 mM phenolic substrate, and 40 to 50 µg of ScCCL . After the reaction mixture had been incubated at 30°C for 5 min, the reaction was initiated by adding CoA and the enzyme . The reaction was carried out at 30°C . The change in absorbance was measured by successive scanning of the wavelength with a Beckman DU7400 spectrophotometer, with the first measurement taken for 2 min . Protein concentrations were determined by the method of Bradford (5) with bovine serum albumin as a standard .

Determination of kinetic parameters. The kinetic constants K_m and k_cat for cinnamate derivatives were estimated by linear regression of 1/v against 1/[S] (Lineweaver-Burk plot) at fixed concentrations of ATP (5 mM) and CoA (0.3 mM) . The concentration of the phenolic substrates was varied from 0.05 to 0.5 mM .

Phylogenetic reconstruction. Proteins similar to adenylate-forming enzymes in a total of 29 sequences were aligned with Clustal W (27) . The phylogenetic tree was constructed by the neighbor-joining method based on multiple alignment with MEGA version 2.1 (S . Kumar, K . Tamura, I . B . Jakobsen, and M . Nei, 2001, Arizona State University) . A total of 490 amino acid sites were considered without gap regions in the alignment . The following sequences were used (the EMBL or GenBank accession numbers are given in parentheses): Arabidopsis thaliana 4CL1 (U18675), A . thaliana 4CL2 (AF106086), A . thaliana 4CL3 (AF106088), aspen 4CL1 (AF041049), aspen 4CL2 (AF041050), Lithospermum 4CL1 (D49366), Lithospermum 4CL2 (D49367), parsley 4CL1 (X13324), parsley 4CL2 (X13325), pine 4CL1 (U12012), pine 4CL2 (U12013), poplar 4CL1 (AF008184), poplar 4CL2 (AF008183), potato 4CL1 (M62755), potato 4CL2a (AAD40664), rice 4CL1 (X52623), rice 4CL2 (L43362), soybean 4CL16 (X69955), tobacco 4CL0 (D43773), tobacco 4CL1 (U50845), tobacco 4CL2 (U50846), vanilla 4CL (X75542), Arabidopsis 4CL-like (chromosome 1) (AC011000), Arabidopsis 4CL-like (chromosome 4-14) (AL161502), Arabidopsis 4CL-like (chromosome 4-49) (AL161549), Hotaria luciferase (AAC37253), Photinus luciferase (CAA59281), Pyrocoelia luciferase (AAC37254), and S . coelicolor A3(2) ScCCL (AL359988) .

 
The information obtained from the crystal structure of the phenylalanine-activating domain of gramicidin S synthase (6) predicted that nine residues formed a substrate-binding pocket in A . thaliana 4CLs (26) (Fig . 1; see also Fig . 4) . Of the nine residues, seven are highly conserved in plant 4CLs . Three (Ile²⁵², Tyr²⁵³, and Pro³⁵⁴) of the seven residues are conserved in ScCCL, but others (Asn²⁵⁶, Gly³²², Gly³⁴⁶, and Val³⁵⁵) are not . The involvement of these residues in the substrate selectivity of ScCCL is discussed below .

 
4CL activity of 4CL-like protein from S . coelicolor A3(2). We cloned the ScCCL gene from the S . coelicolor A3(2) genome by PCR and constructed an expression plasmid, pET-ScCCL, to produce histidine-tagged ScCCL . Improvement of the culture conditions of E . coli BL21(DE3) harboring pET-ScCCL led to production of a considerable amount of the recombinant protein in the soluble fraction (Fig . 2) . The recombinant protein was purified to homogeneity from the soluble fraction with an Ni-nitrilotriacetic acid spin column, giving a single protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Fig . 2) . From 30 ml of culture, about 150 µg of histidine-tagged ScCCL was obtained .

 
With this recombinant protein, we examined the 4CL activity of ScCCL from S . coelicolor A3(2) by the spectrophotometric assay . Under standard conditions used for plant 4CLs, it showed distinct 4CL activity . We first determined the optimum pH and temperature of the 4-coumaroyl-CoA formation catalyzed by ScCCL . The optimum pH was determined to be pH 8.0 when assayed with Tris-HCl buffer . The optimum temperature was determined to be 30°C . These values were similar to those reported for plant 4CLs (19, 20) . We then determined the kinetic constants, K_m for 4-coumarate and k_cat, under the optimum conditions . The K_m for 4-coumarate and k_cat were calculated to be 131 ± 4 µM and 0.202 ± 0.007 s^-1, respectively, on the basis of the Lineweaver-Burk plot (Fig . 3) . The K_m of ScCCL was comparable to those of plant 4CLs; the K_m values for 4-coumarate were 38 µM (A . thaliana 4CL1), 252 µM (A . thaliana 4CL2), and 23 µM (A . thaliana 4CL3) when assayed with recombinant proteins produced in E . coli (10) . Because V_max values per microgram of protein, instead of k_cat values, were described in most cases for plant 4CL-catalyzed reactions and the values were quite different among the individual 4CLs, the k_cat value of ScCCL could not be compared directly with those of plant 4CLs . To our knowledge, this is the first bacterial protein showing distinct 4CL activity .

 
Substrate specificity of ScCCL from S . coelicolor A3(2). The range of substrates used by plant 4CL isoforms varies within and between plant species, but generally 4-coumarate (4-hydroxycinnamate) and caffeate (3,4-dihydroxycinnamate) are the best substrates, followed by ferulate (3-methoxy-4-hydroxycinnamate), with cinnamate itself being a very poor substrate (9) . 4CL activity toward sinapate (3,5-dimethoxy-4-hydroxycinnamate) has been described in a few cases in angiosperms (19, 21), but no such activity of heterologously expressed 4CL isoforms has so far been described .

ScCCL was found to have low activity toward caffeate and no activity toward ferulate (Table 1), both of which are generally good substrates for plant 4CLs . ScCCL also showed no activity toward sinapate . On the other hand, ScCCL efficiently converted cinnamate to the corresponding thiol ester (Table 1), while cinnamate is a very poor substrate for plant 4CLs . The K_m for cinnamate and k_cat values were estimated to be 190 ± 2 µM and 0.475 ± 0.012 s^-1, respectively . The K_m value for cinnamate is 1.5-fold higher than that for 4-coumarate, but the k_cat value for cinnamoyl-CoA formation is 2.4-fold higher than that for 4-coumaroyl-CoA formation, resulting in a 1.7-fold-higher efficiency (k_cat/K_m) for cinnamate than for 4-coumarate . Thus, ScCCL turned out to have a unique substrate specificity .

 
Site-directed mutagenesis of ScCCL and activities of the mutant enzymes. We assumed that the differences in substrate specificity between ScCCL and plant 4CLs could be ascribed to the differences in the amino acid residues that line up along the catalytic pocket, on the basis of the recent study in which the residues important for the substrate specificity of A . thaliana 4CL2 were identified by site-directed mutagenesis (26) . Nine residues probably forming the catalytic pocket have been predicted from the crystal structure of the phenylalanine-activating domain of gramicidin S synthase (6) and the results of the study on A . thaliana 4CLs (26) (Fig . 4) . Two of these residues (Ile²⁵² and Tyr²⁵³; the numbers of the A . thaliana 4CL2 sequence are used) are conserved among all plant 4CLs and ScCCL . Pro³⁵⁴ is also conserved among almost all plant 4CLs (except for potato 4CL2a) and ScCCL . The amino acid residues at positions 293 and 320 varied among plant 4CLs, although the differences are restricted to a few amino acids . The remaining four amino acid residues (Asn²⁵⁶, Gly³²², Gly³⁴⁶, and Val³⁵⁵) are completely conserved in plant 4CLs, whereas the corresponding residues of ScCCL are Thr²²⁸, Ala²⁹⁴, Ala³¹⁸, and Gly³²⁷, respectively .

To determine whether amino acid replacement at these positions changes the substrate specificity of ScCCL, we generated eight ScCCL mutant enzymes (Y265A, A294G, A318G, A294G/A318G, A294G/G327V, A294G/A318G/G327V, T228N/A294G/A318G/G327V, and T228N/Y265A/A294G/A318G/G327V)by using the amino acid residues of A . thaliana 4CL2 at these positions as references (Fig . 4) .

(i) Y265A. Three 4CL isoforms from A . thaliana have different substrate utilization profiles despite their high sequence identity . All three isoforms are able to activate their typical substrates, 4-coumarate and caffeate . However, only A . thaliana 4CL1 and A . thaliana 4CL3 are able to convert ferulate to the corresponding thiol esters, whereas A . thaliana 4CL2 is not (10) . Stuible and Kombrink (26) revealed that the introduction of a small amino acid at either position 293 or 320 promoted ferulate activation by A . thaliana 4CL2 . Furthermore, they revealed that introduction of bulky aromatic amino acids at position 293 of A . thaliana 4CL2 resulted in a 10-fold increase in the K_m value for caffeate (26) .

Because the amino acid residues at both positions 293 and 320 of A . thaliana 4CL2 have been suggested to form the substrate-binding pocket, the bulkiness of the amino acids lining the substrate-binding pocket is an important factor that determines substrate specificity in the 4CL family . In the ScCCL sequence, the amino acid residue corresponding to position 293 of A . thaliana 4CL2 is Tyr²⁶⁵ . We speculated that this bulky aromatic amino acid residue at this position would explain the weak and no activity of ScCCL toward caffeate and ferulate, respectively . Therefore, we generated a mutant ScCCL, Y265A . The K_m value for cinnamate and 4-coumarate of Y265A was decreased, although the k_cat value was also decreased, resulting in a decrease in k_cat/K_m . Contrary to our expectation, however, Y265A could not use caffeate and ferulate as substrates (Table 1) . These results suggested that other amino acid residues would hinder the enzyme's ability to use caffeate and ferulate as substrates .

(ii) A294G, A318G, and A294G/A318G. Judging from the substrate specificity of ScCCL, the substrate-binding pocket of ScCCL is probably smaller than those of plant 4CLs . Therefore, we first replaced Ala²⁹⁴ and Ala³¹⁸, because the corresponding residues in plant 4CLs are Gly, which has a shorter side chain than Ala . A decrease in K_m and an increase in k_cat for cinnamate and 4-coumarate in A294G led to generation of a mutant enzyme with higher catalytic activity than native ScCCL . Probably due to the high catalytic activity, A294G catalyzed the reaction toward caffeate to a detectable extent (Table 1) . Furthermore, A294G/A318G was active toward caffeate and ferulate, although its kinetic parameters for cinnamate and 4-coumarate were similar to those of native ScCCL (Table 1) . This implies that the combination of changes at positions 294 and 318 reduces the K_m value for ferulate . It is noteworthy that A294G and A294G/A318G showed higher catalytic activities toward cinnamate and 4-coumarate than the wild-type enzyme (Table 1) .

(iii) A294G/G327V, A294G/A318G/G327V, T228N/A294G/A318G/G327V, and T228N/Y265A/A294G/A318G/G327V. In contrast to the results with A294G/A318G, the combination of mutations at positions 294 and 327 lowered the catalytic activity even for 4-coumarate . Addition of a mutation at position 318 to A294G/G327V restored the enzyme activity, up to that of A294G, for cinnamate but not for 4-coumarate . These observations suggested that an appropriate combination of amino acid replacements not only of the nine amino acids lining up along the substrate-binding pocket but also of other amino acids forming the pocket and maintaining each of the catalytic residues at correct orientations is necessary to maintain the enzyme activity and change the substrate specificity of ScCCL . Consistent with this idea, the mutant enzymes T228N/A294G/A318G/G327V and T228N/Y265A/A294G/A318G/G327V, which had the same amino acid residues in the substrate-binding pocket as plant-type 4CLs, showed almost no activity toward 4-coumarate .

Phylogenetic reconstruction of 4CLs and 4CL-like proteins. The Arabidopsis genome database (http://www.arabidopsis.org/agi.html) contains at least three 4CL-like genes . The proteins encoded by these genes are similar in size to bona fide 4CLs and are about 40% identical to bona fide 4CLs, with significant similarity over their whole lengths . We generated a phylogenetic tree of 4CLs including these 4CL-like proteins from A . thaliana and the bacterial ScCCL with three luciferases as the outgroup to root the tree (Fig . 5) . The result showed that ScCCL as well as three Arabidopsis 4CL-like proteins was out of a distinct clade containing bona fide 4CLs . Nevertheless, ScCCL was shown to have distinct 4CL activity in this study . This would be important for deducing the possible enzymatic functions of Arabidopsis 4CL-like proteins, although Stuible and Kombrinks (26) reported that one of these proteins (AC011000, 4CL-like chromosome 1 in Fig . 5) showed no 4CL activity .

 
4CLs have been believed to be specific to plants . However, a gene encoding a 4CL-like enzyme which shows more than 40% identity in amino acid sequence to plant 4CLs was found in the genome of the soil-inhabiting, filamentous bacterium S . coelicolor A3(2) . Although this 4CL homologue (ScCCL) was not included in a distinct clade containing bona fide 4CLs in the phylogenetic analyses, we revealed that ScCCL had distinct 4CL activity . To our knowledge, this is the first bacterial protein showing 4CL activity . This finding suggests that proteins having 4CL activity are more widespread than expected .

ScCCL was able to convert cinnamate and 4-coumarate efficiently to the corresponding thiol esters in vitro . The physiological substrate(s) of ScCCL in S . coelicolor A3(2) is not clear, because cinnamate and 4-coumarate are not general metabolites in bacteria . PAL is an essential enzyme for cinnamate and 4-coumarate production from phenylalanine . Although PAL activity in Streptomyces verticillatus was reported more than 30 years ago (3, 12), no gene encoding a PAL homologue is present in the genome of S . coelicolor A3(2) . S . coelicolor A3(2) has a histidine ammonia lyase (histidase), an enzyme closely related to PAL, but PAL activity of the enzyme has not been reported . Therefore, if cinnamate and 4-coumarate are the physiological substrates, they may be supplied from dead plants in the surrounding environment, although no uptake system for cinnamate or 4-coumarate is known in Streptomyces spp .

Speculating on the in vivo function of ScCCL, we point out that four genes which would be involved in the conversion of CoA ester compounds exist in the neighborhood of the ScCCL gene on the S . coelicolor A3(2) genome . These genes encode probable and ß subunits of acetyl/propionyl-CoA carboxylase, a probable acyl-CoA dehydrogenase, and a probable enoyl-CoA hydratase . The termination codon of ScCCL overlaps the initiation codon of the putative enoyl-CoA hydratase gene, which suggests that these two genes are transcriptionally and translationally coupled . Because functionally related genes are usually clustered in the bacterial genome, ScCCL, together with these enzymes, is perhaps involved in the production of some secondary metabolite .

We point out the following biosynthetic pathway in which a 4CL-like enzyme is responsible for secondary metabolite formation . A biosynthetic pathway for benzoyl-CoA, similar to the plant systems, has recently been found in Streptomyces maritimus (15, 22) . The genes involved in this pathway are members in the biosynthesis gene cluster for the enterocin and wailupemycin family of polyketides . Therefore, the pathway would not be ubiquitous in Streptomyces spp . but specific to strains . In this pathway, similar to the plant system, EncH is suggested to be a cinnamoyl-CoA ligase . In contrast to ScCCL, however, EncH shows higher sequence similarity to bacterial acyl-CoA ligases than to plant 4CLs .

ScCCL is quite different from plant 4CLs in its substrate specificity . Three 4CL isoforms from A . thaliana have different substrate utilization profiles despite their high sequence identity . Stuible and Kombrink (26) showed the importance of the bulkiness of the amino acid residues at positions 293 and 320 for the recognition of the 3-methoxy or 3-hydroxy group of the substrate, on the basis of the observation that the introduction of small amino acids at either position 293 or 320 promoted ferulate activation by A . thaliana 4CL2 and that the introduction of bulky aromatic amino acids at position 293 of A . thaliana 4CL2 resulted in a 10-fold increase in the K_m value for caffeate . Because the amino acid residues at both positions 293 and 320 of A . thaliana 4CL2 are suggested to be part of the substrate-binding pocket, the bulkiness of the amino acids lining the substrate-binding pocket is an important factor that determines substrate specificity in the 4CL family .

We therefore focused on determining how the nine amino acid residues that perhaps line up along the substrate-binding pocket might affect substrate specificity . Contrary to our expectation, mutant Y265A, having an amino acid replacement from Tyr to Ala at the position corresponding to 293 of A . thaliana 4CL2, showed low catalytic activity toward cinnamate and 4-coumarate and no activity toward caffeate and ferulate . Although point mutations in these amino acid residues failed to change completely the substrate specificity of ScCCL into those of plant 4CLs, some of the mutants showed different substrate specificities than the wild-type enzyme . A294G and A294G/A318G could convert caffeate more efficiently than the wild-type enzyme, and A294G/A318G could utilize ferulate efficiently, suggesting that Ala²⁹⁴ and Ala³¹⁸ are involved in substrate recognition . Interestingly, the activities of A294G and A294G/A318G for cinnamate and 4-coumarate were increased .

It is surprising that the mutant enzymes with higher activity than the wild-type enzyme were generated, although this may be related to the idea that ScCCL uses certain compounds, different from the compounds used in this study, as a physiological substrate . The discovery of an enzyme having cinnamate/4-coumarate:CoA ligase activity in bacteria is useful for the production of various compounds by combinatorial biosynthesis .

This work was supported by a Research Grant from the Noda Institute for Scientific Research, by a grant from the Industrial Technology Research Grant Program 2000 of the New Energy and Industrial Technology Development Organization of Japan (00A03004), and by the Bio Design Program of the Ministry of Agriculture, Forestry, and Fisheries of Japan (BDP-02-VI-2-7) .

1. Allina, S . M., A . Pri-Hadash, D . A . Theilmann, B . E . Ellis, and C . J . Douglas. 1998 . 4-Coumarate:coenzyme A ligase in hybrid poplar . Plant Physiol . 116:743-754.
2. Becker-André, M., P . Schulze-Lefert, and K . Hahlbrock. 1991 . Structural comparison, modes of expression, and putative cis-acting elements of the two 4-coumarate:CoA ligase genes in potato . J . Biol . Chem . 266:8551-8559.
3. Bezanson, G . S., D . Desaty, A . V . Emes, and L . C . Vining. 1970 . Biosynthesis of cinnamide and detection of phenylalanine ammonia-lyase in Streptomyces verticillatus . Can . J . Microbiol . 16:147-151.
4. Boudet, A . M. 2000 . Lignins and lignification: selected issues . Plant Physiol . Biochem . 38:81-96.
5. Bradford, M . M. 1976 . A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding . Anal . Biochem . 72:248-254.
6. Conti, E., T . Stachelhaus, M . A . Marahiel, and P . Brick. 1997 . Structural basis for activation of phenylalanine in the nonribosomal biosynthesis of gramicidin S . EMBO J . 14:4174-4183.
7. Cukovic, D., J . Ehlting, J . A . VanZiffle, and C . J . Douglas. 2001 . Structure and evolution of 4-coumarate:CoA ligase (4CL) gene family . Biol . Chem . 382:645-654.
8. Dixon, R . A., and N . L . Paiva. 1995 . Stress-induced phenylpropanoid metabolism . Plant Cell 7:1085-1097.
9. Douglas, C . J. 1996 . Phenylpropanoid metabolism and lignin biosynthesis: from weeds to trees . Trends Plant Sci . 1:171-178.
10. Ehlting, J., D . Büttner, Q . Wang, C . J . Douglas, I . E . Somssich, and E . Kombrink. 1999 . Three 4-coumarate:coenzyme A ligases in Arabidopsis thaliana represent two evolutionarily divergent classes in angiosperms . Plant J . 19:9-20.
11. Ehlting, J., J . J . K . Shin, and C . J . Douglas. 2001 . Identification of 4-coumarate:coenzyme A ligase (4CL) substrate recognition domains . Plant J . 27:455-465.
12. Emes, A . V., and L . C . Vining. 1970 . Partial purification and properties of L-phenylalanine ammonia-lyase from Streptomyces verticillatus . Can . J . Microbiol . 48:613-622.
13. Fulda, M., E . Heinz, and F . P . Wolter. 1994 . The fadD gene of Escherichia coli K-12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family . Mol . Gen . Genet . 242:241-249.
14. Hahlbrock, K., and D . Scheel. 1989 . Physiology and molecular biology of phenylpropanoid metabolism . Annu . Rev . Plant Physiol . Plant Mol . Biol . 40:347-369.
15. Hertweck, C., and B . S . Moore. 2000 . A plant-like biosynthesis of benzoyl-CoA in the marine bacterium "Streptomyces maritimus." Tetrahedron 56:9115-9120.
16. Hopwood, D . A., M . J . Bibb, K . F . Chater, T . Kieser, C . J . Bruton, H . M . Kieser, D . J . Lydiate, C . P . Smith, J . M . Ward, and H . Schrempf. 1985 . Genetic manipulation of Streptomyces: a laboratory manual . The John Innes Foundation, Norwich, United Kingdom.
17. Horinouchi, S., and T . Beppu. 1994 . A-factor as a microbial hormone that controls cellular differentiation and secondary metabolism in Streptomyces griseus . Mol . Microbiol . 12:859-864.
18. Hu, W . J., A . Kawaoka, C . J . Tsai, J . H . Lung, K . Osakabe, H . Ebinuma, and V . L . Chiang. 1998 . Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen (Populus tremuloides) . Proc . Natl . Acad . Sci . USA 95:5407-5412.
19. Knobloch, K . H., and K . Hahlbrock. 1975 . Isoenzymes of p-coumarate:CoA ligase from cell suspension cultures of Glycine max . Eur . J . Biochem . 52:311-320.
20. Knobloch, K . H., and K . Hahlbrock. 1977 . 4-Coumarate:CoA ligase from cell suspension cultures of Petroselinum hortense Hoffm . Arch . Biochem . Biophys . 184:237-248.
21. Kutsuki, H., M . Shimada, and T . Higuchi. 1981 . Distribution and roles of p-hydroxycinnamate:CoA ligase in lignin biosynthesis . Phytochemistry 21:267-271.
22. Piel, J., C . Hertweck, P . R . Shipley, D . M . Hunt, M . S . Newman, and B . S . Moore. 2000 . Cloning, sequencing and analysis of the enterosin biosynthesis gene cluster from the marine isolate "Streptomyces maritimus": evidence for the derailment of an aromatic polyketide synthase . Chem . Biol . 7:943-955.
23. Sambrook, J., E . F . Fritsch, and T . Maniatis. 1989 . Molecular cloning: a laboratory manual, 2nd ed . Cold Spring Harbor Laboratory Press, Plainview, N.Y.
24. Stöckigt, J., and M . H . Zenk. 1975 . Chemical synthesis and properties of hydroxycinnamoyl-coenzyme A derivatives . Z . Naturforsch . 30c:352-358.
25. Stuible, H . P., D . Büttner, J . Ehlting, K . Hahlbrock, and E . Kombrink. 2000 . Mutational analysis of 4-coumarate:CoA ligase identifies functionally important amino acids and verifies its close relationship to other adenylate-forming enzymes . FEBS Lett . 467:117-122.
26. Stuible, H . P., and E . Kombrink. 2001 . Identification of the substrate specificity-conferring amino acid residues of 4-coumarate:coenzyme A ligase allows the rational design of mutant enzymes with new catalytic properties . J . Biol . Chem . 276:26893-26897.
27. Thompson, J . D., D . G . Higgins, and T . J . Gibson. 1994 . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position-specific gap penalties and weight matrix choice . Nucleic Acids Res . 22:4673-4680.
28. Uhlmann, A., and J . Ebel. 1993 . Molecular cloning and expression of 4-coumarate:coenzyme A ligase, an enzyme involved in the resistance of soybean (Glycine max) against pathogen infection . Plant Physiol . 102:1147-1156.
29. Weisshaar, B., and G . I . Jenkins. 1998 . Phenylpropanoid biosynthesis and its regulation . Curr . Opin . Plant Biol . 1:251-257.

Free Online Full-text Article

What Is Biofilm?, What Is Yeast?, What Is Bioremediation?, What Is Environmental Microbiology?, What Is Molecular Biology?, o, Microbes, i, Microbiology, a, Bacterium, o, Microorganisms, a, Microbe, o, Bacteriophage, i, Eubacterium, r, Fermentations, c, Microbial, s, Bacillus subtilis, s, Lactobacillus, i, Bacteroides, a, Escherichia coli, s, Meningococcus, s, Bacteroides, s, Escherichia coli, c, Salmonella, c, Haemophilus, i, Cephalosporin, i, Bacillus subtilis, s, Micrococci, c, Antibiotics, c, Streptococcal, n, Cryptococci, e, Antibiotics, n, Antibiotics




 

© 2005 Transgalactic Ltd (manufacturer of Bioscreen C software) | Privacy Statement | P.O. Box 1393, 00101 Helsinki, Finland, phone: +358 9 85172920, fax: +358 9 8749481, e-mail: microbiology@bionewsonline.com
 

Last modified: May 25, 2005