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Cryptosporidium parvum and Giardia lamblia Recovered from Flies on a Cattle Farm and in a Landfill. Beata Szostakowska, 2004.Filth flies associated with a cattle barn and a municipal landfill were tested positive by combined immunofluorescent antibody and fluorescent in situ hybridization for Cryptosporidium parvum and Giardia lamblia on their exoskeletons and in their guts . More pathogens were carried by flies from the cattle barn than from the landfill; 81% of C . parvum and 84% of G . lamblia pathogens were presumptively viable . Complete Nucleotide Sequence of Plasmid Rts1: Implications for Evolution of Large Plasmid Genomes. Takahiro Murata, 2002.Rts1, a large conjugative plasmid originally isolated from Proteus vulgaris, is a prototype for the IncT plasmids and exhibits pleiotropic thermosensitive phenotypes . Here we report the complete nucleotide sequence of Rts1 . The genome is 217,182 bp in length and contains 300 potential open reading frames (ORFs) . Among these, the products of 141 ORFs, including 9 previously identified genes, displayed significant sequence similarity to known proteins . The set of genes responsible for the conjugation function of Rts1 has been identified . A broad array of genes related to diverse processes of DNA metabolism were also identified . Of particular interest was the presence of tus-like genes that could be involved in replication termination . Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes .
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