Antimicrobial Agents and Chemotherapy, July 2004, p . 2751-2752, Vol . 48, No . 7

Comparison of Artesunate and Chloroquine Activities against Plasmodium vivax Gametocytes

Mathieu Nacher,^1,2,3* Udomsak Silachamroon,¹ Pratap Singhasivanon,¹ Polrat Wilairatana,¹ Weerapong Phumratanaprapin,¹ Arnaud Fontanet,² and Sornchai Looareesuwan¹

Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok,¹ Shoklo Malaria Research Unit, Mae Sod, Thailand,² Institut Pasteur, Unite de Recherche et d'Expertise, Epidemiologie des Maladies Emergentes, Paris, France³

Received 25 January 2004/ Returned for modification 14 March 2004/ Accepted 17 March 2004

Between 1993 and 2002, 2,078 patients aged 4 to 83 years were treated at the Hospital for Tropical Diseases in Bangkok for P . vivax malaria . From 1993 to 1998 patients received chloroquine phosphate (Thai Government Pharmaceutical Organization, Bangkok, Thailand) at 10 mg of base/kg of body weight followed by 5 mg of base/kg at h 6, 24, and 36 (n = 1,018) . From 1999 to 2002, patients were treated with artesunate (Guilin No . 1 Factory, Guanxi, People's Republic of China) at 3.3 mg/kg on the first day followed by 1.65 mg/kg for the following 4 days (n = 586) or artesunate at a dose of 1.65 mg/kg/day for 7 days (n = 474) . All patients gave informed consent, and the study was approved by the Ethical Committee of Mahidol University . Patients received oral paracetamol if they had a temperature above 38°C . Among the above patients, 516 (50.7%) of the patients receiving chloroquine and 607 (57.2%) of the patients receiving artesunate received primaquine at the end of their treatment (on the third day for patients receiving chloroquine and on the fifth or seventh day for patients receiving artesunate) to destroy hypnozoites . However, all but four patients (all in the chloroquine group) had already cleared their gametocytes when primaquine was started; therefore, it was inferred that primaquine was given too late to influence the studied outcome . Gametocytes per 200 leukocytes were counted every 12 h by experienced microscopists on Giemsa-stained thick smears until parasitemia became negative . For all patients, a complete blood count was performed on admission, allowing us to transform asexual parasite counts from thin or thick smears into parasitemia expressed in parasites per microliter of blood . The proportion of gametocyte carriers, the peak gametocyte count, and the gametocyte carriage duration were compared between treatment groups . To compare peak gametocyte counts and the proportion of gametocyte carriers between treatment groups, the analysis did not include 591 patients with gametocytes on admission (total number of patients analyzed = 1,487) . The Mann-Whitney test was used to compare medians, and a generalized linear model (binomial family with a log link function) was used to control for the initial parasitemia, age, and fever duration .

After excluding observations of patients with P . vivax gametocytes on admission, patients treated with artesunate were less likely to develop gametocyte carriage (Table 1) . Among patients with P . vivax gametocytes, the median peak gametocyte count was lower in patients treated with artesunate than in those treated with chloroquine, respectively: 465 per mm³ (interquartile range, 305 to 795 per mm³) versus 560 per mm³ (interquartile range, 355 to 975 per mm³) (P = 0.001) . Among patients with P . vivax gametocytes, patients treated with artesunate had detectable gametocytes for a shorter duration than those treated with chloroquine, respectively: median = 24 h (range, 0 to 96 h) versus 24 h (range, 0 to 264 h) (P = 0.005) . The lower gametocyte carriage with artesunate followed a temporal trend for odds (² = 8.94; P = 0.002) . When looking at the difference between artesunate regimens, the 5-day regimen, which delivers a larger initial dose, was associated with less gametocyte carriage than the 7-day regimen risk ratio, 0.83 (95% CI = 0.69 to 0.99; P = 0.04) . All the above results were not affected when controlling for the use of posttreatment primaquine .

 
Gametocytes do not cause any symptoms, but their presence in sufficient numbers and duration is directly responsible for the infection of the anopheline vector, thus perpetuating the plasmodial cycle . Although both chloroquine and artesunate are able to kill gametocytes (5), the treatment of P . vivax malaria with artesunate led to a threefold reduction of gametocyte carriage when compared to chloroquine . Peak gametocyte densities and duration of gametocyte carriage were also lower in patients treated with artesunate . These elements suggest that, as for P . falciparum (2), by reducing transmission, the generalized treatment of P . vivax with artesunate could predictably be followed by a reduction of P . vivax incidence . In addition, hidden P . falciparum infections, which occur in 10% of P . vivax infections (1), could be effectively treated, thus avoiding their delayed appearance following chloroquine treatment . However, although it has been previously shown that artemisinin derivatives had the strongest activity against asexual parasites (3), the problem of relapses was not affected by these drugs and thus still requires the use of primaquine (4) (if the local treatment policy advocates the prevention of relapses) . Thus prospective interventions in target areas should be implemented to measure the potential benefits of artesunate in P . vivax malaria for both individuals and populations .

1. Krudsood, S., P . Wilairatana, D . P . Mason, S . Treeprasertsuk, P . Singhasivanon, and S . Looareesuwan. 1999 . Hidden Plasmodium falciparum infections . Southeast Asian J . Trop . Med . Public Health 30:623-624.
2. Price, R . N., F . Nosten, C . Luxemburger, F . O . ter Kuile, L . Paiphun, T . Chongsuphajaisiddhi, and N . J . White. 1996 . Effects of artemisinin derivatives on malaria transmissibility . Lancet 347:1654-1658.
3. Pukrittayakamee, S., A . Chantra, J . A . Simpson, S . Vanijanonta, R . Clemens, S . Looareesuwan, and N . J . White. 2000 . Therapeutic responses to different antimalarial drugs in vivax malaria . Antimicrob . Agents Chemother . 44:1680-1685.
4. Silachamroon, U., S . Krudsood, S . Treeprasertsuk, P . Wilairatana, K . Chalearmrult, H . Y . Mint, P . Maneekan, N . J . White, V . R . Gordeuk, G . M . Brittenham, and S . Looareesuwan. 2003 . Clinical trial of oral artesunate with or without high-dose primaquine for the treatment of vivax malaria in Thailand . Am . J . Trop . Med . Hyg . 69:14-18.
5. World Health Organization. 2001 . The use of antimalarial drugs: report of an informal consultation . W.H.O . Technical Review W.H.O./CDS/RBM/2001.33 . 43-7 . [Online.] www.emro.who.int/rbm/WHOPositionStatement.pdf.

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