Bio Abstracts

Two Genetic Loci Produce Distinct Carbohydrate-Rich Structural Components of the Pseudomonas aeruginosa Biofilm Matrix.
Lisa Friedman, 2004.Pseudomonas aeruginosa forms biofilms, which are cellular aggregates encased in an extracellular matrix . Molecular genetics studies of three common autoaggregative phenotypes, namely wrinkled colonies, pellicles, and solid-surface-associated biofilms, led to the identification of two loci, pel and psl, that are involved in the production of carbohydrate-rich components of the biofilm matrix . The pel gene cluster is involved in the production of a glucose-rich matrix material in P . aeruginosa strain PA14 (L . Friedman and R . Kolter, Mol . Microbiol . 51:675-690, 2004) . Here we investigate the role of the pel gene cluster in P . aeruginosa strain ZK2870 and identify a second genetic locus, termed psl, involved in the production of a mannose-rich matrix material . The 11 predicted protein products of the psl genes are homologous to proteins involved in carbohydrate processing . P . aeruginosa is thus able to produce two distinct carbohydrate-rich matrix materials . Either carbohydrate-rich matrix component appears to be sufficient for mature biofilm formation, and at least one of them is required for mature biofilm formation in P . aeruginosa strains PA14 and ZK2870 .

Antimicrobial Susceptibility Patterns and Macrolide Resistance Genes of ß-Hemolytic Streptococci in Korea.
Young Uh, 2004.In 540 beta-hemolytic streptococci, the rates of resistance to tetracycline, chloramphenicol, erythromycin, and clindamycin were 80.0, 22.8, 20.2, and 19.1%, respectively . Of the erythromycin-resistant isolates, 63.3% had the constitutive macrolide-lincosamide-streptogramin B (MLS_B) resistance phenotype, 23.9% had the M phenotype, and 12.8% had the inducible MLS_B resistance phenotype . The constitutive MLS_B resistance phenotype with the erm(B) gene was dominant in Korea .

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Last modified: May 25, 2005