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What Is Water Purification?Water purification generally means freeing water from any kind of impurity it contains, such as contaminants or micro organisms. Water purification is not a very one-sided process; the purification process contains many steps. The steps that need to be progressed depend on the kind of impurities that are found in the water. This can differ very much for different types of water. Water purification, or drinking water treatment, is the process of removing contaminants from surface water or groundwater to make it safe and palatable for human consumption. A wide variety of technologies may be used, depending on the raw water source, contaminants present, standards to be met, and available finances. Over time, ecosystems have developed sophisticated ways to process and store natural waste products such as sediments, nutrients, heavy metals, and bacteria. Water is the primary medium for transporting these materials through most ecosystems. The "water purification" process is accomplished to varying degrees in a variety of ecosystems including streams, wetlands, estuaries, and forests. Wetlands and riparian (streamside) plant communities are particularly efficient at improving water quality. These communities act as buffers that filter and process sediment and debris from floods, waste products from aquatic and terrestrial plants and animals, and, minerals, metals, and microorganisms before they can reach faster moving water such as rivers. Sources of Drinking Water Water to be used in public or private water supplies can be drawn from a variety of sources. Different sources of raw water demand different treatment methods to render it ft for human consumption. Deep Groundwater - The water emerging from some deep groundwaters may have fallen as rain many decades or even hundreds of years ago. Soil and rock layers will have naturally filtered the groundwater to a high degree of clarity even before it is pumped to the treatment plant. Such water may emerge as springs, artesian springs, or may be extracted from boreholes or wells. Deep groundwater is generally of very high bacteriological quality but may be rich in dissolved solids especially carbonates and sulphates of calcium and magnesium. Depending on the strata through which the water has flowed , other ions may also be present including chloride, and bi-carbonate and there may be a requirement to reduce the iron or manganese content of this water to make it pleasant for drinking, cooking, and laundry use. Disinfection is also required. Where groundwater recharge is practised, such groundwaters should be graded as being equivalent to lowland surface waters for treatment purposes. Shallow groundwaters - water emerging from shallow groundwaters are usually abstracted from wells or boreholes and the bacteriological quality can be variable depending on the nature of the catchment. A variety of soluble materials may be present including potentially toxic metals such as copper or zinc. In parts of Bangladesh many shallow ground water sources are contaminated with unacceptably high levels of arsenic. Upland lakes and reservoirs - typically located in the headwaters of rivers systems, upland reservoirs are usually sited above any human habitation and may be surrounded by some form of protection zone to restrict the opportunities for contamination. Bacterial and pathogens levels are usually low but some bacteria, protozoa and algae will be present. Where upland are forested or are peaty, humic acids can colour the water brown. Many upland sources have low pH which requires adjustment before the water is put into supply. Rivers , Canals and low-land reservoirs - lowland surface waters will have a significant bacterial load and may also contain algae, suspended solids and a variety of dissolved constituents Water Treatment Methods Screening - A municipal surface water treatment plant must first screen or sieve out large objects such as trash and leaves. The tighter the mesh of the sieve, the smaller the particles must be to pass through. Filtering is not sufficient to completely purify water, but it is often a necessary first step, since such particles can interfere with the more thorough purification methods. Storage- Water from rivers may also be stored in bankside reservoirs for periods between a few days and many months to allow natural biological purification to take place. This is especially important if treatment is to be by slow sand filters The filtered water is then treated to remove any microscopic organisms including protozoa and bacteria. This is generally followed by a disinfection stage to eliminate any residual bacteria and viruses. For waters that are particulalrly difficult to treat such as from catchments with intensive agriculture, both physical and biological treatment methods may be combined. Flocculation -The water is -treated with small volumes of appropriate chemicals which will form a chemical floc which entraps particles. The most common flocculent chemicals are aluminium salts such as aluminium sulfate which is flocculated by the small addition of lime to raise the pH. Rapid Sand Filters - The use of rapid sand filters is the most common form of physical treatment of water. Passing flocculated water through a sand filter strains out the floc and the particles trapped within it. Where taste and odour may be a problem (organo-leptic impacts), the sand filter may include a layer of activated carbon to remove the taste and odour. Sand filters become clogged with floc after a period in use and they are then backwashed or pressure washed to remove the floc. This backwash water is run into special settling tanks so that the floc can precipitate out and is then disposed of as waste material. In some countries this may be used as a soil conditioner. Slow Sand Filters - Where land and space are available, water may be treated in slow sand filter beds. These rely on biological treatment processes for their action rather than physical filtration. Slow sand filters are carefully constructed using graded layers of sand with the coarsest at the base and the finest at the top. Drains buried at the base of the filter convey treated water away for disinfection. When a new slow sand filter bed is brought into use, raw water is carefully decanted onto the filter material until a water depth of 1 to 3m is achieved, dependant on the size of the filter bed. The water passing through the filter for the first few hours is recirculated through the filter and not put into supply. Within a few hours, a biological film comprised of bacteria, protozoa, fungi, and algae builds on the surface of the sand. This is the Schmutzedecke layer and it is this layer that removes all the impurities. An effective slow sand filter may remain in service for many weeks or even months if the pre-treatment is well designed and produces an excellent quality of water which physical methods of treatment rarely achieve. Disinfection - The finished water is then disinfected with chlorine gas, chloramine, sodium hypochlorite, chlorine dioxide, ozone, or ultraviolet light, before it is pumped into the distribution system of water mains and storage tanks on its way to consumers. Some plants also pre-chlorinate their raw water influent after the screening phase to reduce the incidence of biological films in the treatment cycle. Water utilities may choose to further boost chlorine levels (termed re-chlorinating) in the distribution system to counteract any pathogens that may occur. Bleach may be used for emergency disinfection at the rate of 2 drops of 5% bleach per liter or quart of clear water according to a treatment table in the following US EPA document Emergency Disinfection (http://www.epa.gov/safewater/faq/emerg.html). Many environmental and cost considerations affect the siting and design of water purification plants. Groundwater is cheaper to treat, but aquifers once depleted can take thousands of years to recharge. Surface water sources must be carefully monitored for the presence of unusual types or levels of contaminants. The treatment plant itself must be kept secure from vandalism or terrorism and the presence of large quantities of dangerous chemicals mandates special training for workers and emergency personnel. The facility must responsibly dispose of its settled and filtered solids and prevent them from contaminating the treatment components or the source waters. All facilities disinfect finished water, but the exact method of disinfection can be controversial, and the costs and benefits of different methods must be evaluated. Other Water purification techniques Other popular methods for purifying water, especially for local private supplies are listed below. In some countries some of these methods are also used for large scale municipal supply. Particularly important are Distillation (de-salination of sea-water) and reverse osmosis Boiling: Water is heated to its boiling point long enough to inactivate or kill microorganisms that normally live in water at room temperature. Near sea level, bring the water to a vigorous rolling boil for at least one minute. At high altitudes (greater than a kilometer or a mile) boil for three minutes. Source: US EPA (http://www.epa.gov/safewater/faq/emerg.html) In areas where the water is "hard", (containing dissolved calcium salts), boiling decomposes the bicarbonate ion, resulting in some (but not all) of the dissolved calcium being precipitated in the form of calcium carbonate. This is the so-called "fur" that builds up on kettle elements etc. in hard water areas. With the exception of calcium, boiling does not remove solutes of higher boiling point than water, and in fact increases their concentration (due to some water being lost as vapour). Carbon filtering: Charcoal, a form of carbon with a high surface area due to its mode of preparation, adsorbs many compounds, including some toxic compounds. Water is passed through activated charcoal to remove such contaminants. This method is most commonly used in household water filters and fish tanks. Household filters for drinking water sometimes also contain silver, trace amounts of silver ions having a bactericidal effect. Distilling: Distillation involves boiling the water to produce water vapour. The water vapour then rises to a cooled surface where it can condense back into a liquid and be collected. Because the solutes are not normally vaporized, they remain in the boiling solution. Even distillation does not completely purify water, because of contaminants with similar boiling points and droplets of unvaporized liquid carried with the steam. However, 99.9% pure water can be obtained by distillation. Reverse osmosis: Mechanical pressure is applied to an impure solution to force pure water through a semi-permeable membrane. The term is reverse osmosis, because normal osmosis would result in pure water moving in the other direction to dilute the impurities. Reverse osmosis is theoretically the most thorough method of large-scale water purification available, although perfect semi-permeable membranes are difficult to create. Ion exchange: Most common ion exchange systems use a zeolite resin bed and simply replace unwanted ions (Ca2+ and Mg2+) with benign (soap friendly) sodium or potassium ions. This is the common water softener. A more rigorous type of ion exchange swaps hydrogen (H+) ions for unwanted cations and hydroxide (OH-) ions for unwanted anions. The result is H+ + OH- --> H2O. This system is recharged with hydrochloric acid and sodium hydroxide. The result is essentially deionized water. Electrodeionization: Water is passed between a positive electrode and a negative electrode. Ion selective membranes allow the positive ions to separate from the water toward the negative electrode and the negative ions toward the positive electrode. High purity de-ionized water results. The water is usually passed through a reverse osmosis unit first to remove nonionic organic contaminants. Portable water purification Portable drinking water systems or chemical additives are available for hiking, camping, and travel in remote areas. Portable pump filters are commercially available with ceramic filters that will filter 5000 to 50,000 liters per cartridge. Some also utilize activated charcoal filtering. Chemical additives include chlorine dioxide or iodine solutions. Iodine, in solution, crystallized, or in tablets, is added to water. The iodine kills off many, but not all, of the most common pathogens that may be present in natural fresh water sources such as lakes, rivers, and streams. Carrying iodine for water purification is a lightweight but imperfect solution for those in need of field purification of drinking water. Solar Disinfection (Sodis): Microbes are destroyed through temperature and UVA radiation, provided by the Sun. Water is placed in a transparent plastic bottle, which is oxygenated by shaking, followed by topping-up. It is placed on tile or metal for six hours in full sun, which raises the temperature and gives an extended dose of solar radiation, killing any microbes that may be present. The combination of the two provides a simple method of disinfection for tropical developing countries. Water Res, 2004 Nov, 38(19), 4135 - 42Isolation and characterization of a bacterium capable of removing taste- and odor-causing 2-methylisoborneol from water; Lauderdale CV et al.; 2-Methylisoborneol (MIB), a metabolite of blue-green algae, has been implicated in causing unpalatable drinking water throughout the world . Current non-biological water treatment technologies are ineffective in removing MIB from potable water or are cost-prohibitive, and biological applications may address these problems . We have isolated and characterized a bacterium derived from lake water and capable of aerobically degrading MIB . Light microscopy and transmission electron microscopy revealed that this strain is a spore-forming, flagellated bacterium that is bacilloid in shape, and 16S rRNA phylogenetic analysis determined that it is most closely related to Bacillus fusiformis and Bacillus sphaericus, both members of the Bacillus sphaericus senso lato taxon . While the growth and oxidation potential of this strain was shown to be affected beyond certain MIB concentrations in the mg/l range, it was capable of depleting MIB at mg/l and ng/l concentrations and of removing MIB to concentrations yielding no observed odor. Water Res, 2004 Nov, 38(19), 4045 - 54 Performance of ion-exchanger grafted textiles for industrial water treatment in dynamic reactors; Lacour S et al.; The performance of a special class of grafted textiles in removing metal cations from industrial wastewaters was examined in continuous reactors . The influence of various parameters on the ion exchange process (reactor geometry, inlet metal ion concentration, solution flow rate, concentration and type of reagent, etc.) was studied over the complete service cycle of the exchanger (saturation, desorption, regeneration, rinsing) . Dynamic ion exchange characteristics were determined and compared with those of resins under identical operating conditions . Higher efficiency of fibrous ion exchangers compared to analogous resins, was shown at all stages of the service cycle . Results were expressed as a function of breakthrough capacity, exchanger utilisation efficiency, volume of solution treated, eluted metal concentration . The use of different reactor geometries showed two of the many possible applications of grafted textiles in water treatment processes. J Photochem Photobiol B, 2004 Oct 25, 76(1-3), 103 - 6 Sensitised decomposition of microcystin-LR using UV radiation; Gajdek P et al.; UV radiation was applied to degrade cyanobacterial hepatotoxin, microcystin-LR in the presence of phycocyanin as a model natural sensitiser . The concentrations of both the toxin and the pigment used in the experiments were higher by several orders of magnitude than found in the environment . The photoreaction parameters were optimised . The process was found to be of limited use for water treatment due to its low efficacy . Additionally, pronounced UV-induced bleaching of the pigment significantly reducing the photoreaction rates of the toxin was observed for the highest UV radiation intensities applied. Rev Gastroenterol Peru, 2004 Jul-Sep, 24(3), 223 - 9 {Variation of Helicobacter pylori prevalence and its relation with the level of chlorine in the water at the "Atarjea" plant, Lima, PerĂº . Period 1985-2002.}; Ramirez R A et al.; OBJECTIVES: Relate the variation in the prevalence of Helicobacter pylori in patients from medium and high socio-economic levels, suffering from chronic active gastritis (CAG) and peptic ulcer, from 1985 to 2002, in Lima, Peru with the content levels of chlorine in the water at the "Atarjea" Water Treatment Plant . MATERIALS AND METHODS: A total of 1,815 patients showing upper gastrointestinal tract symptoms were examined by esophago-gastro-duodenoscopy, excluding those patients who during the past four weeks had been treated with antibiotics, H2 receptor blockers and proton pump inhibitors . The variation of the H . pylori infection prevalence was compared to the content levels of chlorine in the water at the "Atarjea" plant . RESULTS: A total of 1,290 patients with CAG were identified; 178 with duodenal ulcer; 55 with gastric ulcer and 292 with histologically normal gastric mucose (HNGM) . The prevalence of H . pylori in patients with CAG dropped from 83.3% to 58.7% (p<0.001) . In patients with duodenal ulcer, it decreased from 89.5% to 71.9% (p=0.004) and in those with gastric ulcer, from 84.8% to 77.3% (p=0.36) . In patients with HNGM prevalence remained the same (from 2.7% to 0.0%) (p=0.15) . The chlorine content levels at the water of "Atarjea" plant remained relatively stable from 1991 to 2000 . No correlation was found with the prevalence of Helicobacter pylori (p>0.05) . CONCLUSIONS: Between 1985 and 2002, prevalence of Helicobacter pylori with chronic active gastritis (CAG) and/or peptic ulcer in patients from the medium and upper socio-economic strata, diminished . No correlation was found between the chlorine content levels in the water treated at the "Atarjea" plant and the decrease of this prevalence. Int J Artif Organs, 2004 Aug, 27(8), 723 - 7 The role of improved water quality on inflammatory markers in patients undergoing regular dialysis; Rahmati MA et al.; Hemodialysis utilizes large quantities of water for the preparation of dialysis fluid . Such water meets national standards and international standards but a considerable disparity exists between such standards with respect to microbiological purity . This study collated and retrospectively analyzed the impact of upgrading water systems from that specified in the US standards to those specified in European standards on clinical measures associated with inflammation in four metropolitan dialysis units for two periods . Two periods were compared, three months prior to and six months post upgrading the water treatment systems . The monthly total erythropoietin dosage and intravenous iron supplementation for each patient were also compared over these periods . Variables with significant pre-post differences were assessed using multivariate models to control for confounding factors . The results indicated significant increases in hemoglobin, ferritin and TSat (all p < 0.0001) and albumin (p = 0.0001) were associated with improvement in water quality . Decreases in CRP and creatinine (both p < 0.0001) were also noted . These findings suggest that the current regulations in the United States set the microbiological limits of water and dialysis fluid inappropriately high, and the limits should be revised downwards, since such an approach is reflected in improvement in markers of inflammation. Environ Res, 2005 Jan, 97(1), 116 - 23 Characterization of NOM in the Han River and evaluation of treatability using UF-NF membrane; Kim MH et al.; Chlorine reacts with the natural organic matter (NOM) that remains in waters and forms halogenated by-products . Some of these products such as trihalomethanes (THMs) and haloacetic acids (HAAs) have been known to cause cancer and other toxic effects to human beings . Disinfection is a process for the inactivation of pathogenic microorganisms and prevention of waterborne diseases . Despite these advantages, disinfection needs to be evaluated and is of concern because of the serious risks to human health . Therefore, this study characterized NOM, which is a disinfection by-product (DBP) precursor, in the water treatment processes from the Han River and evaluated the removal efficiencies of NOM fractions by conventional and advanced processes . The raw water from the Han River contained higher hydrophilic fraction than the hydrophobic fraction . The hydrophilic NOM constituted still 55-70% of NOM in waters after each process . Since the hydrophilic NOM exhibited higher HAAFP than hydrophobic NOM, chlorination of the Han River water produced higher haloacetic acid formation potential (HAAFP) than trihalomethane formation potential (THMFP) . When the fractions of DBPFP are expressed as percentage of total organic halide formation potential (TOXFP), THMFP, HAAFP, and others are 26%, 43%, and 31% of the TOXFP, respectively . UF membrane could replace conventional processes showing a little higher removal efficiency of NOM and DBPFP, but advanced processes will be required to meet future requirements . The ultrafiltration (UF)-nanofiltration (NF) combined membrane process had better removal efficiency of NOM compared to other processes . Therefore, the results of this study suggest that the UF-NF membrane process is one of the best available ways for removing NOM. Environ Monit Assess, 2004 Nov, 98(1-3), 421 - 31 Evaluating the efficiency of coagulation in the removal of dissolved organic carbon from reservoir water using fluorescence and ultraviolet photometry; Cheng WP et al.; The present study used ultraviolet absorption (UVa) and the florescence intensity (FI) to evaluate the coagulation efficiency for removing dissolved organic carbon (DOC) in the raw water from Min-Ter, Li-Yu-Ten and Yun-Ho-Shen reservoirs in Taiwan . The results indicated that the ratio of DOC removal rate and FI removal rate was maintained at about 1 at various coagulant dosages . However, the ratio of DOC removal rate and UVa removal rate decreased as the coagulant dosage increased . In addition, after coagulation, the use of florescence intensity instead of total organic carbon (TOC) is better than UVa for measuring the DOC removal rate of the raw waters gathered in different months from the three reservoirs . Furthermore, a good linear relationship between florescence intensity and DOC removal rate was observed, and the DOC/FI ratio of raw water from each reservoir can be used to predict the DOC residual concentration after enhanced coagulation . This result shows that fluorescence analysis can be used for on-line and continuous monitoring the effectiveness of organic matter removal in water treatment. Rev Argent Microbiol, 2004 Apr-Jun, 36(2), 92 - 6 {The action of different coagulants to remove Cryptosporidium during the process of water treatment}; Abramovich B et al.; Cryptosporidium is one of the microorganisms of main concern from the point of view of Public Health, being a priority problem for water treatment plants and water regulatory institutions . Due to its small size and resistance to chlorination, Cryptosporidium removal during the process of drinking water treatment is a hard task . The effectiveness of different coagulants commonly used in the process of removal of oocysts was analyzed . The technique used was the Jar Test . It was found that: 1) coagulants with the addition of polimeric coadjuvants produce over 2 logs of oocyst removal; 2) a low value in turbidity does not necessarily mean optimal parasite removal, and 3) the addition of polyelectrolites to ferric chloride diminishes variability, both in final turbidity and Cryptosporidium removal. Waste Manag Res, 2004 Aug, 22(4), 306 - 22 A historical context of municipal solid waste management in the United States; Louis GE; Municipal solid waste management (MSWM) in the United States is a system comprised of regulatory, administrative, market, technology, and social subcomponents, and can only be understood in the context of its historical evolution . American cities lacked organized public works for street cleaning, refuse collection, water treatment, and human waste removal until the early 1800s . Recurrent epidemics forced efforts to improve public health and the environment . The belief in anticontagionism led to the construction of water treatment and sewerage works during the nineteenth century, by sanitary engineers working for regional public health authorities . This infrastructure was capital intensive and required regional institutions to finance and administer it . By the time attention turned to solid waste management in the 1880s, funding was not available for a regional infrastructure . Thus, solid waste management was established as a local responsibility, centred on nearby municipal dumps . George Waring of New York City organized solid waste management around engineering unit operations; including street sweeping, refuse collection, transportation, resource recovery and disposal . This approach was adopted nationwide, and was managed by City Departments of Sanitation . Innovations such as the introduction of trucks, motorized street sweepers, incineration, and sanitary landfill were developed in the following decades . The Resource Conservation and Recovery Act of 1976 (RCRA), is the defining legislation for MSWM practice in America today . It forced the closure of open dumps nationwide, and required regional planning for MSWM . The closure of municipal dumps caused a 'garbage crisis' in the late 1980s and early 1990s . Private companies assumed an expanded role in MSWM through regional facilities that required the transportation of MSW across state lines . These transboundary movements of MSW created the issue of flow control, in which the US Supreme Court affirmed the protection of garbage under the Commerce Clause of the Constitution . Thus MSWM in America today is largely managed by municipalities, and operated by a relatively small number of private companies . It consists of a mixture of landfill, incineration, recycling, and composting, and is regulated under RCRA, the Clean Air Act and other related federal and state laws. Water Sci Technol, 2004, 50(3), 207 - 15 New method for measuring potential secondary stickies in deinked pulp filtrates; Sarja T et al.; Secondary stickies refer to sticky material in recycled fiber process streams, which has been dissolved in one part of process, but turned to solid phase due to changes in process conditions . The material that can later form secondary stickies is called potential secondary stickies . A new, fast, and simple method for measuring the amount of potential secondary stickies was developed to measure the purification result in the deinked pulp (DIP) filtrates . The new method, now called potential secondary stickies method, was tested with process waters from a recycled fiber (RCF) plant . The method was found to be reliable . Several chemicals and chemical combinations were tested in laboratory scale and compared as coagulation-flocculation agents in two dissolved air flotations (DAF) used as internal water treatment units at the RCF plant . With potential secondary stickies method, significant differences between the effectiveness of the chemicals were found, whereas no difference was seen in the quality of purified process water with traditional measuring methods . The measurement method was also used in mill during a two-week measuring period . As a conclusion, it can be said that this method can be used to monitor the quality and to predict the changes in DIP process water. Wei Sheng Yan Jiu, 2004 Jul, 33(4), 413 - 5 {Development and evaluation of a composite structural filter for removal of arsenic in drinking water}; Zhao Y et al.; OBJECTIVE: To develop a composite structural filter and evaluate its performance on-site for removal of high level arsenic in drinking water . METHODS: Composite filters were manufactured using iron loaded carbon powder and superfine PE resin as main components . Test water was prepared by spiking arsenic in raw drinking water . The filter application safety and removal effects on arsenic and other parameters were tested . RESULTS: The composite structural filter made of iron loaded carbon powder and superfine PE resin in equal quantity was safe for drinking water treatment and reached a capacity of 2.56mgAs/g for water with arsenic concentration of 0.4mg/L, and improved water turbidity and chemical oxygen demand apparently . CONCLUSION: The composite filter manufactured using iron loaded carbon powder and superfine PE resin as main components was appropriate for removal of arsenic in rural drinking water with high level of arsenic. Ground Water, 2004 Sep-Oct, 42(5), 678 - 88 Use of Rhodamine water tracer in the marshland upwelling system; Richardson SD et al.; Rhodamine water tracer (RWT) was used to characterize the migration of waste water within the saline subsurface of a marshland upwelling system (MUS), which is an alternative on-site waste water treatment system for coastal areas . Field tracer studies were performed to investigate the fresh waste water plume movement within the saline ground water . Pore velocities were calculated using first detection times and ranged from 0.68 to 10.7 x 10(-4) cm/sec for the loamy sandy soil matrix present at the site . Use of RWT in the field also allowed determination of main and preferential flowpaths . One- and two-dimensional laboratory experiments were performed using silica sand to investigate the interactions of the organically rich waste water with RWT within the zone surrounding the point of injection (one-dimensional) and the impact of background salinity on plume movement (two-dimensional) . The results from these studies were used to help explain the field data . One-dimensional breakthrough curves revealed retardation factors for the RWT in the waste water mixture of 1.73 to 1.90 . These results were similar to other researchers, indicating little interaction between the waste water and RWT . Variations in pore water salinity (5, 15, 25, and 35 ppt) were found to have a significant effect on pore water velocities of the fresh water plume (two-dimensional), indicating the need to incorporate background salinities into the design process for MUS. Mutagenesis, 2004 Sep, 19(5), 341 - 7 Genotoxicity and cytotoxicity assessment in lake drinking water produced in a treatment plant; Buschini A et al.; Chemical analyses and short-term mutagenicity bioassays have revealed the presence of genotoxic disinfection by-products in drinking water . In this study, the influence of the different steps of surface water treatment on drinking water mutagen content was evaluated . Four different samples were collected at a full-scale treatment plant: raw lake water (A), water after pre-disinfection with chlorine dioxide and coagulation (B), water after pre-disinfection, coagulation and granular activated carbon filtration (C) and tap water after post-disinfection with chlorine dioxide just before its distribution (D) . Water samples, concentrated by solid phase adsorption on silica C18 columns, were tested in human leukocytes and HepG2 hepatoma cells using the comet assay and in HepG2 cells in the micronuclei test . A significant increase in DNA migration was observed in both cell types after 1 h treatment with filtered and tap water, and, to a lesser extent, chlorine dioxide pre-disinfected water . Similar findings were observed for the induction of "ghost" cells . Overloading of the carbon filter, with a consequent peak release, might explain the high genotoxicity found in water samples C and D . Cell toxicity and DNA damage increases were also detected in metabolically competent HepG2 cells treated with a lower concentration of tap water extract for a longer exposure time (24 h) . None of the water extracts significantly increased micronuclei frequencies. l, h. Our monitoring approach appears to be able to detect contamination related to the different treatment stages before drinking water consumption and the results suggest the importance of improving the technologies for drinking water treatment to prevent human exposure to potential genotoxic compounds. J Water Health, 2004 Mar, 2(1), 1 - 13 A systematic review of the health outcomes related to household water quality in developing countries; Gundry S et al.; In developing countries, the microbial contamination of household drinking water is implicated in the prevalence of various diseases . This systematic review is concerned with two health outcomes, general diarrhoea and cholera, and their relationship with water quality at point-of-use . Observational studies investigating this relationship are reviewed, as well as studies of home water treatment and storage interventions . For cholera, a clear relationship was found with contaminated water . Home water treatment and storage interventions were also found to reduce cholera . For general diarrhoea, no clear relationship was found with point-of-use water quality, although interventions did significantly reduce diarrhoeal incidence . Reasons for these apparently contradictory results concerning general diarrhoea are discussed and suggestions for further research offered . The policy implications of the findings are also discussed. J Econ Entomol, 2004 Aug, 97(4), 1378 - 83 Insecticidal properties of a Chenopodium-based botanical; Chiasson H et al.; The emulsifiable concentrate UDA-245 based on an essential oil extract from Chenopodium ambrosioides variety near ambrosioides, a North American herbaceous plant, was compared with commercially available pesticides for their effectiveness to control green peach aphid, Myzus persicae (Sulzer) (Homoptera: Aphididae), western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), and greenhouse whitefly, Trialeurodes vaporariorium (Westwood) (Homoptera: Aleyrodidae) . Side effects on the whitefly parasitoid Encarsia formosa Gahan (Hymenoptera: Aphelinidae) also were determined . With green peach aphid, UDA-245 at 0.5% concentration was significantly more effective than the control (water) treatment in a laboratory bioassay and significantly more effective than neem oil and the control treatment and as effective as insecticidal soap in a greenhouse assay . With the western flower thrips, UDA-245 at 0.5% was significantly more effective than neem oil, insecticidal soap and the control treatment in a laboratory bioassay, whereas in a greenhouse assay, UDA-245 at 1.0% was the only treatment that maintained control of the western flower thrips 2 wk after the last treatment period . UDA-245 at 0.5% (laboratory bioassay) was significantly more effective in managing greenhouse whitefly than neem oil, endosulfan, and the control treatment and as effective as insecticidal soap . Insecticidal soap proved to be toxic to the parasitoid E . formosa (71.9% mortality), whereas UDA-245 at 0.5% was not significantly more toxic than the control (11.2 and 4.6% mortality, respectively) . Our results suggest that a greenhouse integrated pest management (IPM) program using a botanical such as UDA-245 could effectively control infestations of major pests present while having a negligible effect on biological control agents. J Water Health, 2003 Mar, 1(1), 15 - 22 A novel technology to improve drinking water quality: a microbiological evaluation of in-home flocculation and chlorination in rural Guatemala; Rangel JM et al.; An estimated 1 billion persons in low-income countries do not have access to improved drinking water . Chlorine, a useful water treatment agent, is less effective in turbid water, and lacks a visible effect, limiting its acceptability . A product incorporating precipitation, coagulation, flocculation, and chlorination technology (combined product) to reduce microbial, organic and heavy metal contaminants in water was evaluated . The combined product's microbiological efficacy in Guatemalan villagers' households was evaluated . One hundred randomly selected households from four neighboring Guatemalan villages were enrolled . Three groups received the combined product and either the Centers for Disease Control (CDC) water storage vessel, a covered bucket with spigot, or no vessel . One group received chlorine bleach and the CDC water storage vessel, and one group no intervention . Household water samples were collected for 4 weeks and Escherichia coli, chlorine, and turbidity levels were measured . Potable water was defined as having less than one E . coli per 100 ml . Eight (8%) baseline water samples were potable . Follow-up water samples were more likely to be potable than control samples (combined product and traditional vessel 83%; combined product and CDC vessel 92%; combined product and covered bucket with spigot 93%; chlorine and CDC vessel 92%; versus control 5%) . Among combined product users, 98% reported improved water clarity compared with 45% of chlorine bleach users (p < 0.0001) . The combined product technology improved water potability as effectively as chlorine bleach; improved water clarity could motivate more persons to effectively treat their water. J Water Health, 2003 Jun, 1(2), 91 - 100 Indigenous bacterial spores as indicators of Cryptosporidium inactivation using chlorine dioxide; Verhille S et al.; This objective of this study was to explore the practicality of monitoring naturally occurring organisms to predict drinking water treatment plant performance, in this case for the reduction of Cryptosporidium . Surface and ground water from seven drinking water treatment plants across North America that use chlorine dioxide were surveyed for aerobic and anaerobic bacterial spore concentrations . The concentrations of total spores were usually high enough in both raw and treated water to allow 4- to 5-log reductions to be observed across the treatment train by filtering up to 2 l of sample . These results suggested that naturally occurring treatment-resistant spores could be candidates as indicators of treatment performance . However, to be useful as indicators for Cryptosporidium reduction, the organisms would have to exhibit similar resistances to disinfection (chlorine dioxide in this case) in order to be useful . The inactivation kinetics of seven of the most common species were determined, and all were observed to be considerably more susceptible to chlorine dioxide inactivation than Cryptosporidium as reported in the literature . This study therefore did not identify an appropriate ambient microbial indicator for Cryptosporidium control. J Water Health, 2003 Jun, 1(2), 73 - 84 Evaluation of a new water treatment for point-of-use household applications to remove microorganisms and arsenic from drinking water; Souter PF et al.; Contamination of drinking water by microorganisms and arsenic represents a major human health hazard in many parts of the world . An estimated 3.4 million deaths a year are attributable to waterborne diseases . Arsenic poisoning from contaminated water sources is causing a major health emergency in some countries such as Bangladesh where 35 to 77 million people are at risk . The World Health Organization (WHO) has recently recognized point-of-use water treatment as an effective means of reducing illness in developing country households . A new point-of-use water treatment system that is based on flocculation, sedimentation and disinfection was evaluated for the removal of bacterial, viral and parasitic pathogens as well as arsenic from drinking water to estimate its potential for use in developing countries . Tests were conducted with United States Environmental Protection Agency (EPA)-model and field- sample waters from developing countries . Samples were seeded with known numbers of organisms, treated with the combined flocculation/disinfection product, and assayed for survivors using standard assay techniques appropriate for the organism . Results indicated that this treatment system reduced the levels from 10(8)/l to undetectable (<1) of 14 types of representative waterborne bacterial pathogens including Salmonella typhi and Vibrio cholerae . No Escherichia coli were detected post-treatment in 320 field water samples collected from five developing countries . In addition, the water treatment system reduced polio and rotavirus titres by greater than 4-log values . Cyrptosporidium parvum and Giardia lamblia inocula were reduced by greater than 3-log values following use of this water treatment system . Arsenic, added to laboratory test waters, was reduced by 99.8%, and naturally occurring arsenic in field samples from highly contaminated Bangladeshi wells was reduced by 99.5% to mean levels of 1.2 microg/l . This water treatment system has demonstrated the potential to provide improved drinking water to households in developing countries by removing microbial and arsenic contaminants. Water Res, 2004 Nov, 38(18), 3971 - 9 Optimisation and significance of ATP analysis for measuring active biomass in granular activated carbon filters used in water treatment; Magic-Knezev A et al.; A method for determining the concentration of active microbial biomass in granular activated carbon (GAC) filters used in water treatment was developed to facilitate studies on the interactions between adsorption processes and biological activity in such filters . High-energy sonication at a power input of 40 W was applied to GAC samples for the detachment of biomass which was measured as adenosine triphosphate (ATP) . Modelling of biomass removal indicated that a series of six to eight sonication treatments of 2 min each yielded more than 90% of the attached active biomass . The ATP concentrations in 30 different GAC filters at nine treatment plants in The Netherlands ranged from 25 to 5000 ng ATP cm(-3) GAC, with the highest concentrations at long filter run times and pretreatment with ozone . A similar concentration range was observed in nine rapid sand (RS) filters . ATP concentrations correlated significantly (p<0.05) with total direct bacterial cell counts in each of these filter types, but the median value of the ATP content per cell in GAC filters (2.1 x 10(-8) ng ATP/cell) was much lower than in the RS filters (3.6 x 10(-7) ng ATP/cell) . Average biofilm concentrations ranging from 500 to 10(5) pg ATP cm(-2) were calculated assuming spherical shapes for the GAC particles but values were about 20 times lower when the surface of pores >1 microm diameter is included in these calculations . The quantitative biomass analysis with ATP enables direct comparisons with biofilm concentrations reported for spiral wound membranes used in water treatment, for distribution system pipes and other aquatic environments. Water Res, 2004 Nov, 38(18), 3931 - 9 Detection of enteric viruses, Giardia and Cryptosporidium in two different types of drinking water treatment facilities; Ali MA et al.; In this study, two types of drinking water treatment facilities (two conventional drinking water treatment plants (DWTPs) and two compact units (Cus)) were compared referring to their production capacity . Water samples were collected from three main points: (a) different water treatment steps (b) washings of sand filters and (c) distribution system at different distances from the water treatment plants . Both viruses and protozoa were concentrated from each water sample by adsorption and accumulation on the same nitrocellulose membrane filters (0.45 microm pore size) . Enteroviruses were detected by plaque infectivity assay in BGM cells and HAV, HEV and Norovirus were detected by RT-PCR . Giardia and Cryptosporidium were detected by conventional staining methods and PCR . The results revealed that enterovirus load at the intake ranged between 10-15 PFU/L for the two compact units and between 4.5 and 75 PFU/L for the two conventional DWTPs . The virus load in distribution system of the first type DWTPs at 1 km from the plant was the same as that of the intake . Viruses in the other type of treatment plants CUs at 1, 5 and 7 km, were much reduced . Investigation of raw water sediments of the two DWTPs showed enterovirus counts between 12 and 17.5 PFU/L . Virus count was reduced in sand of filters after washing . Giardia cysts were equally detected by microscopy and PCR in only intake samples of EL-Hawamdia CU (33.3%) and Meet Fares DWTP (50%) . Cryptosporidium oocysts were equally detected by microscopy and PCR in intake samples of Abo EL-Nomros CU (100%), EL-Hawamdia CU (66.7%) and Fowa DWTP (50%) . At Meet Fares DWTP three positive intake samples for Cryptosporidium were detected by PCR, compared with only two positive samples by microscopy. g, e. Giardia cysts and Cryptosporidium oocysts were detected in raw water sediment and sand of filters before washing . Only one sample from Meet Fares DWTP sand of filters after washing was positive for both Giardia and Cryptosporidium . It can be concluded that the poor microbial quality of the water may be due to improper operational skills and management of the various water treatment plants (especially at the two high capacity treatment plants). J Colloid Interface Sci, 2004 Nov 1, 279(1), 1 - 22 Theorization on ion-exchange equilibria: activity of species in 2-D phases; Tamura H; Ion-exchange reactions are naturally occurring at soil and sediment/water interphases, determining soil fertility and water quality . These ion-exchange reactions with inorganic and organic exchangers are applied to chemical analysis, recovery of useful ions from low-grade ores (potentially from sea water), water purification including the preparation of "ultrapure" water, production of foods and medicines, therapy, and other uses . It is important to theorize about or to model ion-exchange reactions for quantitative explanations of ion-exchange phenomena and for efficient operation of ion-exchange processes . This paper describes the modeling of ion-exchange equilibria for hydroxyl sites on metal oxides and carboxyl sites in resins with monovalent cations (alkali metal ions), a monovalent anion (nitrate ion), and divalent heavy metal ions . The procedure of modeling is as follows: the stoichiometry and material balance equations of the respective ion-exchange reactions were established based on findings here and by others . The equilibrium conditions were given by the Frumkin equation, where the mass-action relation is modified with lateral interactions between species at the interphase . The model equations were fitted to the measured data and model parameter values were determined by nonlinear regression analysis . The formation of bonds between ions and exchanger sites was evaluated by the equilibrium constant and the suppression of bond formation by electrostatic, geometric, and other lateral interactions was evaluated by the interaction constant . It was established that the properties of ions are determined by the valence, size, and hydration state of the ions . Monovalent ions (anions and cations) react with oxide surface hydroxyl and resin carboxyl sites as hydrated ions and form loose ion-site pairs by a weak electrostatic bond (nonspecific adsorption) . However, the lateral interactions are large because of a large polarization of the ion-site pairs . When the monovalent cations are dehydrated to react with carboxyl sites in narrow resin nanopores, the bond formation is difficult because energy for dehydration is necessary . The suppressive lateral interactions here are small because of a small polarization of the dehydrated ion-site pairs that are in direct contact . Divalent heavy metal ions react with oxide hydroxyl sites by replacing their hydrated water molecules and form ion-site pairs in direct strong contact (specific adsorption) . The bond formation becomes easier with increasing charge density of the ions evaluated by the charge/radius ratio, agreeing with the order of these ions to form hydroxo complexes in solution . The suppressive lateral interaction is, however, small for ions with large charge densities, because a strong contact bond reduces the polarization of ion-site pairs by neutralization . The properties of exchangers are functions of the molecular and pore environments around the functional groups . The acid-base nature of oxide surface-hydroxyl groups is determined by the electronegativity of surrounding lattice metal ions, and that of resin carboxyl groups by the electron-repelling effect of adjacent methyl groups . Pores in oxides have diameters sufficient to accommodate hydrated ions, and the suppression is large because of repulsion from ions adsorbed on opposite pore walls (across-pore interaction) . Pores in resins differentiate ions that can access or not access sites on the internal surfaces of the pores . Narrow nanopores with diameters less than those of the hydrated ions require ions to dehydrate before they can enter . The ion-exchange reactivity here is small, as described above for dehydrated monovalent ions . In wide nanopores where hydrated ions can enter, bond formation is easier, but suppression is greater because of a larger polarization of hydrated ion-site pairs and also of the across-pore interaction . Macropores have diameters much larger than those of the hydrated ions and the bond formation is the same as that in wide nanopores, but the suppression is smaller because of the absence oe of the absence of the across-pore interaction . Finally, this paper attempts a formulation of activity coefficients of exchanging sites and adsorbed ion-site pairs and compares the proposed activity coefficients of interphase species with that of solution species given by the Debye-Huckel equation. J Chromatogr A, 2004 Aug 6, 1045(1-2), 85 - 92 Monitoring of estrogens, pesticides and bisphenol A in natural waters and drinking water treatment plants by solid-phase extraction-liquid chromatography-mass spectrometry; Rodriguez-Mozaz S et al.; A multi-residue analytical method has been developed for the determination of various classes of selected endocrine disruptors . This method allows the simultaneous extraction and quantification of different estrogens (estradiol, estrone, estriol, estradiol-17-glucuronide, estradiol diacetate, estrone-3-sulfate, ethynyl estradiol and diethylstilbestrol), pesticides (atrazine, simazine, desethylatrazine, isoproturon and diuron), and bisphenol A in natural waters . In the method developed, 500 ml of water are preconcentrated on LiChrolut RP-18 cartridges . Further analysis is carried out by liquid chromatography-mass spectrometry (LC-MS) using atmospheric pressure chemical ionisation (APCI) in the positive ion mode for determination of pesticides and electrospray in the negative ionisation mode for determination of estrogens and bisphenol A . Recoveries for most compounds were between 90 and 119%, except for bisphenol A (81%) and diethylstilbestrol (70%), with relative standard deviations below 20% . Limits of detection ranged between 2 and 15 ng/l . The method was used to study the occurrence of the selected pollutants in surface and groundwater used for abstraction of drinking water in a waterworks and to evaluate the removal efficiency of the different water treatments applied . Water samples from the river, the aquifer, and after each treatment stage (sand filtration, ozonation, activated carbon filtration and post-chlorination) were taken monthly from February to August of 2002 . The presence in river water of atrazine, simazine, diuron and bisphenol A were relatively frequent at concentrations usually below 0.1 microg/l . Lower levels, below 0.02 microg/l, were usual for isoproturon . Estrone-3-sulfate and estrone were detected occasionally in the river . Most of the compounds were completely removed during the water treatment, especially after activated carbon filtration. J Nephrol, 2004 Jul-Aug, 17(4), 565 - 9 Dialysis water treatment systems and monitoring in Italy: results of a national survey; Pizzarelli F et al.; BACKGROUND: The risks connected with dialysis fluid contamination are well established . Epidemiological studies have demonstrated just how difficult it is to obtain permanently satisfactory quality standards . Therefore, dialysis centers must have effective and regular quality control programs, particularly with the growing and widespread use of online convective dialysis treatments . Considering this, we conducted a national survey of water treatment systems and monitoring in Italian dialysis centers . METHODS: Two independent questionnaires were prepared and distributed in 1999 and 2001 . The questions were designed to acquire information on structural and procedural elements . Responses to similar questions from both questionnaires were analyzed together . RESULTS: Responses to questionnaires were received from 148 centers (17% of dialysis centers in Italy) . In accordance with the European Best Practice Guidelines (EBPG) published recently concerning dialysis fluid purity, in the majority of centers (97%) the water treatment system consisted of at least pre-treatment and reverse osmosis (RO) modules . However, only one stage RO module was implemented (71%), there was a water storage tank (65%) and water pipe distribution loop was made of sanitary polyvinyl chloride (85%) . Analysis of procedural elements--the timing and type of disinfection procedures used for the waterline system and monitors, the timing and type of water treatment quality control procedures--revealed a striking variability among centers in terms of the types and frequency of checks performed . CONCLUSIONS: Taking into account the EBPG, this survey revealed both bright and dark spots, calling for a national initiative aimed at defining a standard periodicity of loop disinfection, points in the water pipe line to be tested, and the frequency and type of tests. J Toxicol Environ Health A, 2004 Oct 22-Nov 26, 67(20-22), 1797 - 803 Disinfection by-products in small Alberta community drinking-water supplies; Charrois J et al.; Complacent attitudes toward drinking-water quality can lead to compromised disinfection practices, as noted in such episodes as Walkerton and North Battleford . The first priority for drinking-water providers must be to ensure microbial safety . However, it is recognized that effective disinfection may not be risk free . Consequently, drinking-water guidelines seek to balance the certain danger posed by microbial pathogens with the potential adverse health hazards that may arise from disinfection by-products (DBPs) . Providers of drinking water in small communities often do not have the means to reduce concentrations of DBPs, compared to utilities in larger municipalities . A significant portion of Alberta's population receives drinking water from smaller scale treatment plants or from private wells . A survey of selected DBPs was conducted in 11 rural Alberta communities, with populations ranging from 60 to 2300 . The objectives were to evaluate source water quality, as measured by total organic carbon, and to measure representative concentrations of trihalomethanes (THMs) and haloacetic acids (HAAs) at a point within each distribution system as well as within each water treatment plant . During the 5-wk study, our data show: (1) averages of THM3 (chloroform, bromodichloromethane, chloro dibromomethane) concentrations often exceed 100 microg/L (Health Canada's running annual average guideline for total THMs); (2) source waters with the highest TOC concentrations (15 mg/L) had the highest average THM3 concentrations (200 microg/L); and (3) poor source water quality may necessitate using alternative disinfection options to ensure compliance with microbial and chemical drinking-water guidelines. J Toxicol Environ Health A, 2004 Oct 22-Nov 26, 67(20-22), 1779 - 95 Aquatic taste and odor: a primary signal of drinking-water integrity; Watson S; Aquatic taste and odor (T/O) is rarely produced by toxic contaminants or pathogens; nevertheless, it has major negative impacts on the public and the drinking-water industry . Consumers use T/O as a primary measure of drinking water safety, yet this criterion is poorly understood, and its origins and triggers often go untraced . Much surface-water T/O is produced by the increased production of volatile organic compounds (VOCs) by algae . These chemicals can be symptomatic of short-term problems with source, treatment, or distribution systems . At a broader level, they can signify fundamental changes in aquatic ecosystems induced by human activity . T/O varies in chemistry, intensity, and production patterns among different algal taxa, and is often linked with excessive algal growth and/or the invasion of noxious species . Some VOCs may signal the presence of potentially toxic algae and/or other associated water quality issues . Traditionally, T/O has been linked with the widespread eutrophication of many surface waters; however, there has been a recent growth in the number of T/O events reported in oligo-mesotrophic systems, for example, the Glenmore Reservoir (Calgary AB) and the Laurentian Great Lakes . From a management and public perspective, therefore, it is vitally important to monitor T/O, and to continue to work toward a better understanding of the proximal and the ultimate causes-which VOCs and algae species are involved . In the short term, odor events could be anticipated and water treatment optimized . In the long term, this approach would contribute toward more a robust management of this resource through remedial or preventative measures. J Toxicol Environ Health A, 2004 Oct 22-Nov 26, 67(20-22), 1619 - 42 Rural water safety from the source to the on-farm tap; Corkal D et al.; For those Canadians who live in metropolitan areas, good quality water for domestic use and consumption is readily available, and perhaps taken for granted . However, for over 4 million Canadians who rely on private water supplies, access to water that is safe for consumption and suitable for domestic use is a very real issue . This is also true in the agriculture and agri-food sector . Many of these private water supplies are in rural areas, where water is taken from surface or ground sources . These supplies may be of naturally poor quality, or may have had their quality affected by municipal, industrial, or agricultural activities . Options to protect and enhance the quality of private water supplies include source protection using best management practices (BMPs), source enhancement, and water treatment using innovative small-scale systems . With funding from the Canada-Saskatchewan Agri-Food Innovation Fund, Agriculture and Agri-Food Canada has conducted applied research into effective and affordable BMPs (remote livestock watering, low-drift nozzles for spraying farm chemicals, methods to reduce agro-chemical runoff or leaching, etc.) . Source enhancement strategies were studied using aeration for farm ponds or preventive maintenance procedures for ground water wells . Various water treatment technologies were adapted to the small-scale needs of farms, including coagulation, biological sand and biological carbon filtration, membrane filtration using microfilters, nanofilters or reverse osmosis processes, and disinfection systems using chlorination or ultra violet light . Each research project included a technology transfer component, to ensure that the knowledge gained from the research was available to those that needed the information, and to help decision makers address rural water quality problems. Bioresour Technol, 2003 Oct, 90(1), 1 - 9 Solids removal in upflow anaerobic reactors, a review; Mahmoud N et al.; This desk study deals with the mechanisms and parameters affecting particles separation from wastewater in mainly upflow anaerobic reactors . Despite the fact that the functioning of upflow anaerobic sludge blanket (UASB) systems depends on both physical parameters and biological processes, the physical parameters have been barely reported in the literature . The reason is that the underlying mechanisms are very complex and depend on various interrelated parameters . In addition, the lack of a serious attempt to gather the entire physical theme into one picture has resulted in just a superficial understanding of this field of science . Better understanding of the interaction and role of these parameters is essential for the development of anaerobic treatment technologies . In this study, the various parameters that might affect the solid liquid separation process by filtration through the sludge bed of a UASB have been elaborated . These parameters have been classified into (1) reactor operational conditions (temperature, organic loading rate, hydraulic retention time and upflow velocity), (2) influent characteristics (influent concentration, influent particle size and influent particle charge) and (3) sludge bed characteristics (particle size distribution, extracellular polymeric substances, and charge) . The overall output of this study includes (1) a literature review, (2) structuring of this field of science, and (3) highlighting fields where research is needed. J Hazard Mater, 2003 Jun 27, 100(1-3), 163 - 78 Treatment of textile wastewaters by electrocoagulation using iron and aluminum electrodes; Kobya M et al.; Treatment of textile wastewaters by electrocoagulation using iron and of aluminum electrode materials has been investigated in this paper . The effects of relevant wastewater characteristics such as conductivity and pH, and important process variables such as current density and operating time on the chemical oxygen demand (COD) and turbidity removal efficiencies have been explored . Furthermore, the electrode and energy consumptions for each electrode have been calculated . The results show that iron is superior to aluminum as sacrificial electrode material, from COD removal efficiency and energy consumption points. J Environ Monit, 2003 Jun, 5(3), 384 - 94 Multi-component analysis of polar water pollutants using sequential solid-phase extraction followed by LC-ESI-MS; Loos R et al.; A multi-component screening analysis method for polar to medium-polar water pollutants was developed . Sample clean-up and group separation are performed by sequential solid-phase extraction (SSPE) using automated SPE with C18 and polymeric sorbent materials . Analyses are performed by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) using a single-quadrupole instrument . More than 90 priority compounds of environmental interest--comprising the most important chemical and substance classes: phenols, carboxylic acids, aromatic sulfonates, aromatic amines, pharmaceuticals, surfactants, dyes, and pesticides--have been chosen for the experiments . The compounds are divided by the SSPE procedure into 3 different polarity classes . The extraction recoveries were determined in the 3 fractions for every single substance, and were for most of the analytes in the range of 50-100% . A mixture of hexane-dichloromethane was used for the elution of nonpolar compounds like alkylphenols from C18 . Methanol and acetone are well suited for the elution of more polar substances . The limits of detection (LODs) were determined for all compounds . Effluents from municipal and industrial wastewater treatment plants (WWTPs) treating waste water from textile industries; and the corresponding receiving waters (rivers and lakes) have been analysed with the developed method . Urban and industrial pollution was observed in rivers and streams in the area north of Milan, Italy . In the water samples different phenols (nitrophenols, bisphenol A, nonylphenol), alkylphenol ethoxylate surfactants, their metabolites with endocrine disrupting potential, aromatic sulfonates, linear alkylbenzenesulfonate surfactants, dyes, pesticides, pharmaceuticals, and a dichlorobenzidine compound were identified. J Chromatogr A, 2002 Mar 1, 948(1-2), 129 - 38 Preparation and characterization of polyalkene membranes modified with four different ion-exchange groups by radiation-induced graft polymerization; Lee KP et al.; Three different cation-exchange membranes with carboxylic acid, phosphoric acid, and sulfonic acid groups, and one anion-exchange membrane with a triethylamine group were prepared by radiation-induced graft polymerization . The maximum capacities of the carboxylic acid, phosphoric acid, sulfonic acid, and triethylamine groups were 3.0, 2.30, 2.43, and 1.42 mmol/g, respectively . The physico-chemical properties of the polyalkene non-woven fabric membrane with four different ion-exchange groups were examined by thermogravimetric analysis, X-ray photoelectron spectrometry (XPS), and scanning electron microscopy . The prepared ion-exchange membrane was applied to the removal of terephthalic acid in dyeing wastewater. Water Sci Technol, 2003, 47(9), 173 - 8 Electrochemical pilot scale study for reduction of 2,4-DNT; Doppalapudi R et al.; An electrochemical pilot scale reactor was used to treat simulated munitions wastewater containing 100 mg/L of 2,4-dinitrotoluene (DNT) . Experiments were conducted by using a glassy carbon (zero porosity) coated graphite cylinder as the cathode and a platinum wire as the anode . All experiments were conducted under dissolved oxygen concentration of less than 1.5 mg/L . Initially, simulating batch conditions were conducted to obtain the optimum operating conditions for the reactor . During this batch-mode study, the effects of various parameters such as applied current, electrolyte concentration, and type of electrolyte on the reduction of DNT were evaluated . Results obtained showed that the rates of reduction of DNT increased with an increase in current or concentration of electrolyte . Based on the results obtained from the batch simulation experiments, continuous flow experiments were conducted at three different currents . The ionic strength of the feed solution was maintained at 0.027 M. f, j, b. A current of 200 mA was found to provide a stable reduction of DNT at the 80% level for a period of 14 days after which reactor cleaning is necessary for removal of solids that were formed within the reactor . End products determined for the continuous flow experiments showed 100% molar balance conversion. Water Sci Technol, 2003, 47(9), 137 - 42 Heterogeneous ultrasonic destruction of aqueous organic contaminants; Suri RP et al.; Sonolysis is emerging as an effective advanced technology for destruction of organic pollutants in wastewater . The focus of this study is to investigate the potential of enhancing the sonochemical destruction of aqueous organics . Increasing the contaminant destruction rate will reduce the reaction time, and possibly reduce the cost of treatment . For this purpose, the effect of H2O2 and/or silica in the presence of ultrasound is examined for destruction of 2-chlorophenol (2-CP) . The effect of silica dosage (1, 5, 10, and 20 g/L), peroxide dosage (60, 75, and 100 mg/L), and pH (3, 7, and 11) is examined . Low solution pH provided higher destruction of 2-CP . The presence of peroxide or silica enhanced the destruction of 2-CP . The optimum silica dosage was 5 g/L . 100 mg/L of peroxide and 5 g/L of silica present together enhanced 2-CP sonolytic destruction by a factor of approximately 2 as compared to 2-CP destruction with ultrasound only. Water Sci Technol, 2003, 47(9), 71 - 6 Evaluating bioaccumulation of suspected endocrine disruptors into periphytons and benthos in the Tama River; Takahashi A et al.; There are two major routes through which fish are exposed to endocrine disruptors (EDs); one route is through water that is a habitat; the other is through aquatic food such as algae and benthos . Few studies on the bioaccumulation of EDs in food have been conducted . Therefore, we evaluated the concentration in food of nonylphenol (NP), bisphenol A (BPA) and 17beta-estradiol (E2), which were frequently detected in river water and in final discharge of Wastewater Treatment Plants (WWTPs) in Japan . We also evaluated the estrogenicity of samples using recombinant yeast . NP concentrations ranged 0.1-0.4 microg/L in the river water, while they ranged 8-130 microg/kg-wet in the periphytons and 8-140 microg/kg-wet in the benthos . BPA concentrations ranged 0.02-0.15 microg/L in the river water, while they ranged 2-8.8 microg/kg-wet in the periphytons and 0.3-12 microg/kg-wet in the benthos . E2 concentrations ranged 0.0001-0.0076 microg/L in the water, while they ranged 0.09-2.26 microg/kg-wet in the periphytons and <0.01-0.22 microg/kg-wet in the benthos . The estrogenicity ranged 0.0001-0.0464 microg-E2equivalent/L in the water, while it ranged 3.4-66.8 microg-E2equivalent/kg-wet in the periphytons and 7.4-5458 microg-E2equivalent/kg-wet in the benthos . Bioaccumulation factors of NP are estimated as 160-650 for the periphytons, and 63-990 for the benthos, respectively . Bioaccumulation factors of BPA are estimated as 18-650 for the periphytons, and 8-170 for the benthos, respectively . Bioaccumulation factors of E2 are estimated as 64-1,200 for the periphytons, and 100-160 for the benthos, respectively . The ratios of the periphytons and the benthos to the water in terms of the estrogenicity were larger than those in terms of the chemicals . In particularly, the ratio of the benthos to the water is about 10(6) in the maximum . The results suggest that food may be a more important route for fish exposed to EDs in water environment. Water Sci Technol, 2003, 47(9), 51 - 7 The effect of chlorination of estrogenic chemicals on the level of serum vitellogenin of Japanese medaka (Oryzias latipes); Tabata A et al.; Mature male medaka were continually exposed to four chemicals, p-n-nonylphenol (p-n-NP), nonylphenol (p-NP), bisphenol-A (BPA) and 17beta-estradiol (E2) to evaluate their estrogenic activities in the laboratory . In order to understand the effect of the chlorination that is applied widely in water and wastewater treatment, the above chemicals were chlorinated and then exposed to mature male medaka . Furthermore, in the case of vitellogenin, a is a female specific protein induced by the exposure to test waters containing the above chemicals after 5 weeks, medaka was returned to uncontaminated tap water to determine whether male medaka have a self recovery function from the effect of estrogenic chemicals . Much greater vitellogenin compared to the background levels were induced in the male medaka by separate exposure to 100 microg/L of p-NP, 1,000 microg/L of BPA and 0.05 microg/L of E2 . The levels of vitellogenin increased with increasing exposure periods . The relative potencies of these chemicals descended in the order of E2>>p-NP>BPA . Vitellogenin levels inducible by these chemicals were drastically reduced as a result of the chlorination for 24 hours . However, a moderate increase in hepatocyte somatic index (HSI) meant the hepatic fatness was observed as a result of chlorination . It is not clear at this stage whether or not the formation of chlorination byproducts is responsible for this moderate increase in HSI . The vitellogenin concentration of male medaka exposed to chemicals for 5 weeks decreased gradually after return to the uncontaminated water . However, the vitellogenin concentration did not return to the initial normal levels even after 5 weeks . A clear relationship between the serum vitellogenin concentration and the hepatic vitellogenin concentration was also found . Since quantitative analytical procedures for hepatic vitellogenin are easier than those of the serum vitellogenin, measuring the estrogenic effect using the measurement of vitellogenin in liver is recommended. FEMS Microbiol Lett, 2003 Jun 27, 223(2), 287 - 92 Extracellular polymeric substances responsible for bacterial adhesion onto solid surface; Tsuneda S et al.; The influence of extracellular polymeric substances (EPS) on bacterial cell adhesion onto solid surfaces was investigated using 27 heterotrophic bacterial strains isolated from a wastewater treatment reactor . Cell adhesion onto glass beads was carried out by the packed-bed method and the results were discussed in terms of the amount of each EPS component produced and cell surface characteristics such as zeta potential and hydrophobicity . Protein and polysaccharides accounted for 75-89% of the EPS composition, indicating that they are the major EPS components . Among the polysaccharides, the amounts of hexose, hexosamine and ketose were relatively high in EPS-rich strains . For EPS-poor strains, the efficiency of cell adhesion onto glass beads increased as the absolute values of zeta potential decreased, suggesting that electrostatic interaction suppresses cell adhesion efficiency . On the other hand, the amounts of hexose and pentose exhibited good correlations with cell adhesiveness for EPS-rich strains, indicating that polymeric interaction due to the EPS covering on the cell surface promoted cell adhesion . It was concluded that, if the EPS amount is relatively small, cell adhesion onto solid surfaces is inhibited by electrostatic interaction, and if it is relatively large, cell adhesion is enhanced by polymeric interaction. Environ Monit Assess, 2003 Jun, 85(2), 115 - 34 Benchmarking for best practice environmental management; Jenkins BR et al.; Benchmarking of environmental performance to demonstrate the achievement of best practice environmental management is a component of a new form of licensing of industrial discharges in Western Australia . The paper describes the approaches to benchmarking for the critical environmental issues for an alumina refinery and wastewater treatment plant . It also describes the lessons learnt from the benchmarking process on appropriate methods, the benefits and difficulties in the benchmarking process, and changes that would assist benchmarking for best practice environmental management. Environ Pollut, 2003, 125(3), 385 - 92 Kinetics of basic dye (methylene blue) biosorption by giant duckweed (Spirodela polyrrhiza); Waranusantigul P et al.; Wastewater containing pigments and/or dyes can cause serious water pollution problems in the form of reduced light penetration and photosynthesis, and the toxicity from heavy metals associated with pigments and/or dyes . Laboratory investigations, of the potential use of dried Spirodela polyrrhiza biomass as an adsorbent for the removal of the basic dye methylene blue from aqueous solution were conducted . A series of experiments were undertaken in an agitated batch adsorber to assess the effect of the system variables, i.e . sorbent dosage, pH, and contact time . The results showed that as the amount of the dried S . polyrrhiza increased, the percentage of dye sorption increased accordingly . At pH 2.0 the sorption of dye was not favorable, while the sorption at other pHs (3.0-11.0) was remarkable . There was no significant difference in the dye concentration remaining when the pH was increased from 3.0 to 11.0 . The dye removal time was influenced by the initial dye concentration, and the process followed the first-order rate kinetics . The rate constants for intraparticle diffusion were 1.00 and 3.27 mg/g/min1/2 for 300 and 500 mg/l of dye, respectively. Sci Total Environ, 2003 Jul 1, 310(1-3), 47 - 59 Trends of phosphorus, nitrogen and chlorophyll a concentrations in Finnish rivers and lakes in 1975-2000; Raike A et al.; During recent decades the amounts of nutrients discharged to Finnish surface waters have markedly decreased . This has been achieved by considerable investments in water protection, which were made mainly to improve municipal and industrial wastewater purification . We investigated whether these water protection measures have decreased phosphorus and nitrogen concentrations in Finnish rivers and lakes . In addition, possible trends in chlorophyll a concentrations in lakes were studied . The data consisted of a total of over 68000 monitoring results of 22 rivers and 173 lakes (or sub-basins of lakes) with different types of catchment areas . The study period covered the years 1975-2000 and the non-parametric Kendall Tau b and Seasonal Kendall tests were applied for detecting trends . Decreasing nutrient concentration trends were typical in many lakes and rivers earlier polluted by municipal and industrial wastewaters . Increasing nutrient concentration trends were common in smaller rivers and lakes receiving diffuse loading from agriculture . The results show that the investments directed towards wastewater purification have effectively improved the quality of Finnish inland waters . However, no clear effects of decreasing non-point loading were found . Thus, more effective measures should be directed towards decreasing non-point source loading. J Environ Qual, 2003 May-Jun, 32(3), 1122 - 9 Sequestration of phosphorus by acid mine drainage floc; Adler PR et al.; Solubilization and transport of phosphorus (P) to the water environment is a critical environmental issue . Flocs resulting from neutralizing acid mine drainage (AMD) were tested as a possible low-cost amendment to reduce the loss of soluble P from agricultural fields and animal wastewater . Flocs were prepared by neutralizing natural and synthetic solutions of AMD with limestone, lime, ammonium hydroxide, and sodium hydroxide . Phosphorus sequestration was tested in three distinct environments: water, soil, and manure storage basins . In water, flocs prepared from AMD adsorbed 10 to 20 g P kg(-1) dry floc in equilibrium with 1 mg L(-1) soluble P . Similar results were observed for both Fe-based and Al-based synthetic flocs . A local soil sample adsorbed about 0.1 g P kg(-1), about two orders of magnitude less . The AMD-derived flocs were mixed with a high-P soil at 5 to 80 g floc kg(-1) soil, followed by water and acid (Mehlich-1) extractions . All flocs performed similarly . About 70% of the water-extractable P was sequestered by the floc when applied at a rate of 20 g floc kg(-1) soil, whereas plant-available P only decreased by about 30% . Under anaerobic conditions simulating manure storage basins, all AMD flocs reduced soluble P by greater than 95% at a rate of 0.2 g floc g(-1) rainbow trout (Oncorhynchus mykiss) manure . These findings indicate that AMD flocs could be an effective agent for preventing soluble P losses from soil and manure to the water environment, while at the same time decreasing the costs associated with AMD treatment. ScientificWorldJournal, 2002 Jun 20, 2, 1689 - 98 Can early life-stages of the marine fish Sparus aurata be useful for the evaluation of the toxicity of linear alkylbenzene sulphonates homologues (LAS C10-C14) and commercial LAS? Hampel M, Moreno-Garrido I, Blasco J. Most commercial household cleaning agents and personal care products contain the anionic surfactant linear alkylbenzene sulphonates (LAS) as the active compound . After their use they are discharged, theoretically after adequate wastewater treatment, into receiving waters finally reaching estuaries and coastal waters . Laboratory toxicity tests are useful tools in determining at which concentration a certain wastewater compound becomes hazardous for an existing group of organisms . Early life-stage toxicity tests include exposure during the most sensitive development period of the organism . In fish, this type of assay has shown to predict accurately maximum acceptable toxicant concentration (MATC) values (comprised in the range defined by the NOEC and LOEC) in fish early life-stage tests . For this reason, larvae of the seabream, Sparus aurata, were exposed to increasing concentrations of LAS homologues (C10-C14) and commercial LAS . Obtained LC50 values ranged between 0.1 and 3.0 mg l(-1) and were compared with LC50 values of previous hatching experiments with the same species . Larvae proved to be more sensitive to LAS exposure of individual homologues than eggs, except in the case of commercial LAS . LC50 values can be directly employed to determine their potential risk in a concrete environment with known pollutant concentrations . Dividing the LC50 value with the found homologue concentration and extrapolating with certain security factors proposed by different environmental organisms, potentially hazardous pollutant concentrations may be detected . Average estuarine or coastal LAS concentrations are generally below toxicity limits for this kind of organism, considering that the average alkyl chain length of commercial LAS is 11.6 carbon atoms. ScientificWorldJournal, 2002 Jan 05, 2, 48 - 52 Wastewater minimization in indirect electrochemical synthesis of phenylacetaldehyde; Sun Z et al.; Wastewater minimization in phenylacetaldehyde production by using indirect electrochemical oxidation of phenylethane instead of the seriously polluting traditional chemical process is described in this paper . Results show that high current efficiency of Mn(III) and high yield of phenylacetaldehyde can be obtained at the same sulfuric acid concentration (60%) . The electrolytic mediator can be recycled and there will be no waste discharged. ScientificWorldJournal, 2002 Apr 26, 2, 1121 - 6 A decontamination process to remove metals and stabilise Montreal sewage sludge; Mercier G et al.; The Montreal Urban Community (MUC) treatment plant produces approximately 270 tons of dry sludge daily (tds/day) during physicochemical wastewater treatment . The sludges are burned and contribute to the greenhouse effect by producing atmospheric CO2 . Moreover, the sludge emanates a nauseating odour during its thermal stabilisation and retains unpleasant odours for the part (25%) that is dried and granulated . To solve this particular problem, the treatment plant authorities are currently evaluating an acidic chemical leaching (sulfuric or hydrochloric acid) process at a pH between 2 and 3, using an oxidizing agent such as ferric chloride or hydrogen peroxide (METIX-AC technology, patent pending; {20}) . They could integrate it to a 70 tds/day granulated sludge production process . Verification of the application of METIX-AC technology was carried out in a pilot plant set up near the sludge production plant of the MUC . The tests showed that METIX-AC technology can be advantageously integrated to the process used at the MUC . The residual copper (274 +/- 58 mg/kg) and cadmium (5.6 +/- 2.9 mg/kg) concentrations in the treated sludge meet legislation standards . The results have also shown that odours have been significantly eliminated for the dewatered, decontaminated, and stabilized biosolids (> 97%) compared to the non-decontaminated biosolids . A high rate of odour elimination also was obtained for the liquid leached biosolids (> 93%), compared to the untreated liquid biosolids . The fertilising value (N and P) is well preserved by the METIX-AC process . Dissolved organic carbon measurements have showed that little organic matter is brought in solution during the treatment . In fact, the average concentration of dissolved organic carbon measured in the treated liquid phase is 966 +/- 352 mg/l, whereas it is 1190 +/- 325 mg/l in untreated sludge . The treated sludge was first conditioned with an organic polymer and a coagulant aid . It was successfully dewatered with various dehydration equipments (filter press, rotary press, centrifuge). ScientificWorldJournal, 2001 Oct 23, 1 Suppl 2, 908 - 13 ThermoEnergy Ammonia Recovery Process for municipal and agricultural wastes; Fassbender AG; The Ammonia Recovery Process (ARP) is an award-winning, low-cost, environmentally responsible method of recovering nitrogen, in the form of ammonia, from various dilute waste streams and converting it into concentrated ammonium sulfate . The ThermoEnergy Biogas System utilizes the new chemisorption-based ARP to recover ammonia from anaerobically digested wastes . The process provides for optimal biogas production and significantly reduced nitrogen levels in the treated water discharge . Process flows for the ammonia recovery and ThermoEnergy biogas processes are presented and discussed . A comparison with other techniques such as biological nitrogen removal is made . The ARP technology uses reversible chemisorption and double salt crystal precipitation to recover and concentrate the ammonia . The ARP technology was successfully proven in a recent large-scale field demonstration at New York City's Oakwood Beach Wastewater Treatment Plant, located on Staten Island . This project was a joint effort with Foster Wheeler Environmental Corporation, the Civil Engineering Research Foundation, and New York City Department of Environmental Protection . Independent validated plant data show that ARP consistently recovers up to 99.9% of the ammonia from the city's centrate waste stream (derived from dewatering of sewage sludge), as ammonium sulfate . ARP technology can reduce the nitrogen (ammonia) discharged daily into local bodies of water by municipalities, concentrated animal farming operations, and industry . Recent advances to ARP enhance its performance and economic competitiveness in comparison to stripping or ammonia destruction technologies. ScientificWorldJournal, 2001 Oct 09, 1 Suppl 2, 802 - 8 Multicompartment ecosystem mass balances as a tool for understanding and managing the biogeochemical cycles of human ecosystems; Baker LA et al.; Nitrogen remains a ubiquitous pollutant in surface and groundwater throughout the United States, despite 30 years of pollution control efforts . A detailed multicompartment N balance for the Central Arizona-Phoenix ecosystem is used to illustrate how an ecosystem-level approach can be used to develop improved N management strategies . The N balance is used to demonstrate how nitrate in pumped groundwater used for crop irrigation could be used to reduce inputs of commercial fertilizer and decrease N leaching to aquifers . Effectively managing N pollution also will require an understanding of the complex factors that control the N balance, including targeted regulations, individual human behavior, land-use conversion, and other ecosystem management practices that affect the N balance . These sometimes countervailing factors are illustrated with several scenarios of wastewater treatment technology and population growth in the Phoenix area . Management of N eventually must be coupled to management of other elements, notably carbon, phosphorus, and salts . We postulate that an ecosystem framework for pollution management will result in strategies that are more effective, fairer, and less expensive than current approaches. ScientificWorldJournal, 2001 Nov 22, 1 Suppl 2, 976 - 83 A potential integrated water quality strategy for the Mississippi River Basin and the Gulf of Mexico; Greenhalgh S et al.; Nutrient pollution, now the leading cause of water quality impairment in the U.S., has had significant impact on the nation"s waterways . Excessive nutrient pollution has been linked to habitat loss, fish kills, blooms of toxic algae, and hypoxia (oxygen-depleted water) . The hypoxic "dead zone" in the Gulf of Mexico is one of the most striking illustrations of what can happen when too many nutrients from inland watersheds reach coastal areas . Despite programs to improve municipal wastewater treatment facilities, more stringent industrial wastewater requirements, and agricultural programs designed to reduce sediment loads in waterways, water quality and nutrient pollution continues to be a problem, and in many cases has worsened . We undertook a policy analysis to assess how the agricultural community could better reduce its contribution to the dead zone and also to evaluate the synergistic impacts of these policies on other environmental concerns such as climate change . Using a sectorial model of U.S . agriculture, we compared policies including untargeted conservation subsidies, nutrient trading, Conservation Reserve Program extension, agricultural sales of carbon and greenhouse gas credits, and fertilizer reduction . This economic and environmental analysis is watershed-based, primarily focusing on nitrogen in the Mississippi River basin, which allowed us to assess the distribution of nitrogen reduction in streams, environmental co-benefits, and impact on agricultural cash flows within the Mississippi River basin from various options . The model incorporates a number of environmental factors, making it possible to get a more a complete picture of the costs and co-benefits of nutrient reduction . These elements also help to identify the policy options that minimize the costs to farmers and maximize benefits to society. Environ Technol, 2003 May, 24(5), 589 - 96 Environmental assessment of cement/foundry sludge products; Ruiz MC et al.; This work deals with the environmental assessment of products based on cement and a waste from a cast iron activity . The waste is a foundry sludge from wastewater treatment previously characterized . This industrial waste shows a high water content (62.4%) and a hazardous behavior due to its metallic content mainly Zn (16.5%), together with a low fraction of organic pollutants, mainly phenolic compounds . The feasibility of immobilizing both typs of contaminants was studied using Portland cement as binder at different cement/waste ratios . The parameters of environmental control were the ecotoxicity and mobilization of zinc and phenolic compounds, all determined on the basis of compliance leaching tests . The acid neutralization capacity of the cement/waste products was measured in order to obtain information on their buffering capacity . Experimental results from chemical analysis of leachates led to a non ecotoxic character of cement/waste products Although the metallic ions were mobilized within the cement mattices, the organic matter did not allow the formation of monolithic forms and an efficient immobilization of phenolic compounds. a, d, b. Concerning the acid neutralization capacity, this parameter was shown to depend mainly on the quantity of cement, although a decrease in alkalinity was observed when the amount of water in the cement/waste products increased. Environ Technol, 2003 May, 24(5), 561 - 72 Evaluation of a single-residence biological wastewater treatment system with thermal biosolids destruction; Wistrom AO et al.; An aerobic package residential wastewater treatment system was evaluated to determine process performance while connected to a single-family residence . Three components of the study were: 1) long-term (5-month) performance, 2) 24-hour performance track studies, and 3) enhanced loading study . For the long-term performance and enhanced loading study hourly influent and effluent samples were collected and composited on a flow-averaged basis . Short-term fluctuations in flow and mass loading rates were evaluated from the hourly discrete samples . The long-term average removals for CBOD5, TSS, and inorganic N were 97.3, 97.8 percent, and 89.6 percent, respectively, with average effluent concentrations of 5.7, 5.9, and 2.2 mg l(-1) . To prevent biosolids buildup in the system and to eliminate the need for frequent sludge removal a thermal process is incorporated into the system for biosolid ash conversion . Periodic spikes of influent mass loading rates persisted in the treatment system at time scales of one hour or less . Treatment process peformance, evaluated from 24-hour composite samples, was not affected by the highly variable infuent concentrations and flow rates experienced in the study single-family household . Average monthly power consumption for aeration, thermal processing of accumulated biosolids, and biomass re-circulation was 132 kWh per month, or about 4.4 kWh per day. Appl Microbiol Biotechnol, 2004 Feb, 63(5), 602 - 8 Epub 2003 Jun 12. Changes in structure, activity and metabolism of aerobic granules as a microbial response to high phenol loading; Jiang HL et al.; Four column-type sequential aerobic sludge blanket reactors were fed with phenol as the sole carbon and energy source and operated at loading rates of 1.0, 1.5, 2.0 and 2.5 kg phenol m(-3) day(-1) . The results indicated that phenol loading exerted a profound influence on the structure, activity and metabolism of the aerobic granules . Compact granules with good settling ability were maintained at loadings up to 2.0 kg phenol m(-3) day(-1), and structurally weakened granules with enhanced production of extracellular polymers and proteins and significantly lower hydrophobicities were observed at the highest loading of 2.5 kg phenol m(-3) day(-1) . Specific oxygen uptake rate, catechol 2,3-dioxygenase (C23O) and catechol 1,2-dioxygenase (C12O) activities peaked at a loading of 2.0 kg phenol m(-3) day(-1), and declined thereafter . Granules degraded phenol completely in all four reactors, mainly through the meta cleavage pathway as C23O activities were significantly higher than C12O activities . At the highest loading applied, the anabolism and catabolism of microorganisms were regulated such that phenol degradation proceeded exclusively via the meta pathway, apparently to produce more energy for overstimulation of protein production against phenol toxicity . This work contributes to a better understanding of the ability of aerobic granules to handle high-strength industrial wastewaters containing chemicals that are normally inhibitory to microbial growth. J Chromatogr A, 2003 May 2, 995(1-2), 87 - 97 Optimization, validation and comparison of various extraction techniques for the trace determination of polycyclic aromatic hydrocarbons in sewage sludges by liquid chromatography coupled to diode-array and fluorescence detection; Miege C et al.; There is a need for a better characterization of sludges from wastewater treatment plants which are destined to be spread on agricultural lands . Inorganic pollutants are regularly controlled but organic pollutants have received few attention up to now . On this paper, we have been interested on the analysis of the 16 polycyclic aromatic hydrocarbons (PAHs) listed in the US Environmental Protection Agency (US EPA) priority list and more particularly of the six PAHs listed in the European community list (fluoranthene, benzo{b and k}fluoranthene, benzo{a}pyrene, benzo{ghi}perylene, indeno{1,2,3-cd}pyrene) . The analysis step consists on liquid chromatography with both fluorescence and UV detections as described in the EPA Method 8310 . As for the extraction step, several techniques such as supercritical fluid extraction, pressurized liquid extraction, focused microwave extraction in open vessels, Soxhlet and ultrasonic extractions are compared after optimization of the experimental conditions (solvent nature and quantity, temperature, pressure, duration, .. . ) and validation with certified sludges . When optimized, these five extraction techniques are as much efficient with similar relative standard deviation . Whatever the extraction techniques used, the whole analysis protocol permits to quantify PAHs in the range of 0.09 to 0.9 mg/kg of dried sludges. Huan Jing Ke Xue, 2003 Mar, 24(2), 130 - 3 {Study on two stage immerged biological film wastewater treatment reactor}; Tian G et al.; The two stage immerged biological film wastewater treatment reactor was studied in different organic loads . The organic removal efficiency of each stage, filter capability and technical contrast between one stage reactor and two stages reactor were discussed . The results showed that the distribution ratio of organic removal were 76% and 24% in first and second stage reactors respectively, COD removal efficiency was 42% in sand filter unit, and the two stage reactor was more effective and reliable than one stage reactor in the same organic loads. Huan Jing Ke Xue, 2003 Mar, 24(2), 121 - 4 {Advanced electrochemical oxidation process for treatment of biorefractory wastewater containing typical aromatic compounds}; Zhou M et al.; Biorefractory wastewater containing one kind of typical aromatic compounds, i.e., aniline, chlorobenzene, p-chlorophenol and p-nitrophenol, was investigated by Advanced Electrochemical Oxidation Process (AEOPs) on a novel beta-PbO2 anode modified by fluorine resin . Under current of 0.25A, the removal rate for organic compounds and COD of the wastewater in 2 hours was around 75%-100%, and 22%-55%, respectively, following by the sequence of aniline, chlorobenzene, p-chlorophenol and p-nitrophenol . Higher current and initial organic compound concentration could enhance the wastewater treatment efficiencies . Benzoquinone, fumaric acid and oxalic acid were detected as the common degradation intermediates by HPLC, based on which a general degradation pathway of these aromatic compounds was further proposed. Huan Jing Ke Xue, 2003 Mar, 24(2), 116 - 20 {Experimental study on the treatment of organic wastewater containing chlorobenzene by using an EGSB reactor}; Wang Y et al.; In this paper, the study on the inhibition and recovery of the methanogenic activity of the granular sludge which uncontacted and contacted with chlorobenzene caused by chlorobenzene, and the study of the treatment of organic wastewater containing chlorobenzene by using an EGSB reactor were conducted . The results showed that the activity of the granular sludge which had not contacted with chlorobenzene would be inhibited by different concentrations chlorobenzene; but for the granular sludge which had contacted with chlorobenzene, its activity would be inhibited only by higher concentration (100 mg/L) chlorobenzene . Using EGSB reactor to treat organic wastewater containing chlorobenzene, when the influent chlorobenzene concentration was about 10-50 mg/L, the effluent concentration was lower than 7 mg/L before 65 days, but after the 66th day, the effluent concentration suddenly reached above 25 mg/L, and after stopping adding chlorobenzene, the effluent chlorobenzene concentration was between 3.4-38.32 mg/L, this showed that the high chlorobenzene removal efficiency was mainly due to the strong adsorption by granular sludge, and the biological degradation of chlorobenzene was not obvious. J Chromatogr A, 2002 Jul 19, 963(1-2), 205 - 11 Chemically facilitated chromium(VI) transport throughout an anion-exchange membrane application to an optical sensor for chromium(VI) monitoring; Castillo E et al.; The Raipore R1030 membrane, an anion-exchange membrane containing ammonium groups as ionogenic groups, was evaluated as the interface of an optical sensor for Cr(VI), and the effect of chemical parameters affecting Cr(VI) transport were studied . Good transport features were obtained, demonstrating the suitability of the Raipore R1030 membrane for this application . Thus, an optical sensor for chromium(VI) monitoring in industrial process waters was developed . The sensor is based on the renewable reagent approach and uses the Raipore R1030 membrane as the interface between the sample and the sensor head, which contains 1,5-diphenylcarbazide as spectrophotometric reagent for chromium . Chromium(VI) crosses the membrane and reacts with the reagent inside the sensor head, resulting in changes in the absorption of light . These changes are monitored in situ through a system of optical fibers . The sensor performance was tested by analysing samples from a waste water treatment plant for effluents from electroplating industries. BMC Public Health . 2002 Aug 15;2(1):13. Identification of bacteria in drinking and purified water during the monitoring of a typical water purification system; Penna VT et al.; BACKGROUND: A typical purification system that provides purified water which meets ionic and organic chemical standards, must be protected from microbial proliferation to minimize cross-contamination for use in cleaning and preparations in pharmaceutical industries and in health environments . METHODOLOGY: Samples of water were taken directly from the public distribution water tank at twelve different stages of a typical purification system were analyzed for the identification of isolated bacteria . Two miniature kits were used: (i) identification system (api 20 NE, Bio-Merieux) for non-enteric and non-fermenting gram-negative rods; and (ii) identification system (BBL crystal, Becton and Dickson) for enteric and non-fermenting gram-negative rods . The efficiency of the chemical sanitizers used in the stages of the system, over the isolated and identified bacteria in the sampling water, was evaluated by the minimum inhibitory concentration (MIC) method . RESULTS: The 78 isolated colonies were identified as the following bacteria genera: Pseudomonas, Flavobacterium and Acinetobacter . According to the miniature kits used in the identification, there was a prevalence of isolation of P . aeruginosa 32.05%, P . picketti (Ralstonia picketti) 23.08%, P . vesiculares 12.82%,P . diminuta 11.54%, F . aureum 6.42%, P . fluorescens 5.13%, A . lwoffi 2.56%, P . putida 2.56%, P . alcaligenes 1.28%, P . paucimobilis 1.28%, and F . multivorum 1.28% . CONCLUSIONS: We found that research was required for the identification of gram-negative non-fermenting bacteria, which were isolated from drinking water and water purification systems, since Pseudomonas genera represents opportunistic pathogens which disperse and adhere easily to surfaces, forming a biofilm which interferes with the cleaning and disinfection procedures in hospital and industrial environments. J Food Prot, 2002 Aug, 65(8), 1276 - 80 Effectiveness of electrolyzed water as a sanitizer for treating different surfaces; Park H et al.; The effectiveness of electrolyzed (EO) water at killing Enterobacter aerogenes and Staphylococcus aureus in pure culture was evaluated . One milliliter (approximately 10(9) CFU/ml) of each bacterium was subjected to 9 ml of EO water or control water (EO water containing 10% neutralizing buffer) at room temperature for 30 s . Inactivation (reduction of > 9 log10 CFU/ ml) of both pathogens occurred within 30 s after exposure to EO water containing approximately 25 or 50 mg of residual chlorine per liter . The effectiveness of EO water in reducing E . aerogenes and S . aureus on different surfaces (glass, stainless steel, glazed ceramic tile, unglazed ceramic tile, and vitreous china) was also evaluated . After immersion of the tested surfaces in EO water for 5 min without agitation, populations of E . aerogenes and S . aureus were reduced by 2.2 to 2.4 log10 CFU/ cm2 and by 1.7 to 1.9 log10 CFU/cm2, respectively, whereas washing with control water resulted in a reduction of only 0.1 to 0.3 log10 CFU/cm2 . The washing of tested surfaces in EO water with agitation (50 rpm) reduced populations of viable cells on the tested surfaces to < 1 CFU/cm2 . For the control water treatment with agitation, the surviving numbers of both strains on the tested surfaces were approximately 3 log10 CFU/cm2 . No viable cells of either strain were observed in the EO water after treatment, regardless of agitation . However, large populations of both pathogens were recovered from control wash solution after treatment. Curr Opin Biotechnol, 2002 Jun, 13(3), 218 - 27 Bacterial community composition and function in sewage treatment systems; Wagner M et al.; The application of modern molecular techniques has led to the identification, in situ quantification, and partial ecophysiological characterisation of bacteria responsible for bulking and foaming or for nutrient removal in sewage treatment systems . Unexpectedly, previously unrecognised, yet uncultured bacteria were demonstrated to catalyse nitrogen and phosphorous removal in activated-sludge and biofilm reactors . These findings provide the basis for the development of novel concepts for improving the efficiency and functional stability of waste water treatment systems. Urban Health Newsl, 1997 Mar, (32), 29 - 33 Water fluoridation; Rudolph MJ; PIP: This article discusses the importance of fluoridation of the water supply in South Africa, as a racially and socioeconomically equitable contribution to health, and for building a healthy nation . Costs of fluoridation amount to under R1/person/year, which is 18 times cheaper than toothpaste and 61 times cheaper than filling a tooth cavity . The dental profession and health professionals are faced with the challenge of informing elected officials and the total population about improving community oral health through fluoridation . Many people are confused about the effectiveness of water fluoridation . The health care establishment can be charged with negligence for not informing the public about the benefits of fluoridation as a safe public health measure that is effective and economical . A recent survey found that under 25% of the survey population had heard or read about water fluoridation . Among those with knowledge of fluoridation, almost 60% learned from mass media reports . Only 25% could identify the purpose of fluoridation, and 40% associated fluoridation with water purification . Only 13% opposed fluoridation for reducing tooth decay . It is suggested that fluoridation be introduced through legislative and administrative action at the national, provincial, and local levels . Collaboration should occur in related sectors . Section 37 of the Health Act No . 63 (1977) authorizes the Minister of Health to establish regulations on fluoridation of public water supplies . The new National Health Bill also provides for water fluoridation . Many governmental bodies approve of fluoridation . J Environ Qual, 2002 Jul-Aug, 31(4), 1388 - 98 Particulate and dissolved phosphorus chemical separation and phosphorus release from treated dairy manure; Dao TH et al.; In confined animal feeding operations, liquid manure systems present special handling and storage challenges because of the large volume of diluted wastes . Water treatment polymers and mineral phosphorus (P) immobilizing chemicals {AI2(SO4)3 x 18H2O, FeCl3-6H2O, and Class C fly ash} were used to determine particulate and dissolved reactive phosphorus (DRP) reduction mechanisms in high total suspended solid (TSS) dairy manure and the P release from treated manure and amended soils . Co-application exceeded the aggregation level achieved with individual manure amendments and resulted in 80 and 90% reduction in metal salt and polymer rates, respectively . At marginally effective polymer rates between 0.01 and 0.25 g L(-1), maximal aggregation was attained in combination with 1 and 10 g L(-1) of aluminum sulfate (3 and 30 mmol Al3+ L(-1)) and iron chloride (3.7 and 37 mmol Fe3+ L(-1)) in 30 g L(-1) (TSS30) and 100 g L(-1) TSS (TSS100) suspensions, respectively . Fly ash induced particulate destabilization at rates > or = 50 g L(-1) and reduced solution-phase DRP at all rates > or = 1 g L(-1) by 52 and 71% in TSS30 and TSS100 suspensions, respectively . Aluminum and Fe salts also lowered DRP at rates < or = 10 g L(-1) and higher concentrations redispersed particulates and increased DRP due to increased suspension acidity and electrical conductivity . The DRP release from treated manure solids and a Typic Paleudult amended with treated manure was reduced, although the amendments increased Mehlich 3-extractable P . Therefore, the synergism of flocculant types allowed input reduction in aggregation aid chemicals, enhancing particulate and dissolved P separation and immobilization in high TSS liquid manure. J Environ Qual, 2002 Jul-Aug, 31(4), 1362 - 9 Influence of water treatment residuals on phosphorus solubility and leaching; Elliott HA et al.; Laboratory and greenhouse studies compared the ability of water treatment residuals (WTRs) to alter P solubility and leaching in Immokalee sandy soil (sandy, siliceous, hyperthermic Arenic Alaquod) amended with biosolids and triple superphosphate (TSP) . Aluminum sulfate (Al-WTR) and ferric sulfate (Fe-WTR) coagulation residuals, a lime softening residual (Ca-WTR) produced during hardness removal, and pure hematite were examined . In equilibration studies, the ability to reduce soluble P followed the order Al-WTR > Ca-WTR = Fe-WTR >> hematite . Differences in the P-fixing capacity of the sesquioxide-dominated materials (Al-WTR, Fe-WTR, hematite) were attributed to their varying reactive Fe- and Al-hydrous oxide contents as measured by oxalate extraction . Leachate P was monitored from greenhouse columns where bahiagrass (Paspalum notatum Flugge) was grown on Immokalee soil amended with biosolids or TSP at an equivalent rate of 224 kg P ha(-1) and WTRs at 2.5% (56 Mg ha(-1)) . In the absence of WTRs, 21% of TSP and 11% of Largo cake biosolids total phosphorus (PT) leached over 4 mo . With co-applied WTRs, losses from TSP columns were reduced to 3.5% (Fe-WTR), 2.5% (Ca-WTR), and <1% (Al-WTR) of applied P . For the Largo biosolids treatments all WTRs retarded downward P flux such that leachate P was not statistically different than for control (soil only) columns. l, a, i, j. The phosphorus saturation index (PSI = {Pox}/ {Al(ox) + Fe(ox)}, where Pox, Al, and Fe(ox) are oxalate-extractable P, Al, and Fe, respectively) based on a simple oxalate extraction of the WTR and biosolids is potentially useful for determining WTR application rates for controlled reduction of P in drainage when biosolids are applied to low P-sorbing soils. Water Res, 2002 Jul, 36(12), 3161 - 4 Low- and medium-pressure UV inactivation of microsporidia Encephalitozoon intestinalis; Huffman DE et al.; Newly recognized waterborne pathogens such as microsporidia are being detected in the world's water supplies with increasing frequency . Many of these organisms have been shown to cause negative health impacts for both immunocompetent as well as immunocompromised individuals . It is imperative that these emerging pathogens be investigated for their ability to resist both traditional and novel disinfection technologies that are currently in use or under consideration for drinking water treatment . Low- and medium pressure UV light is at the cutting edge of disinfection technologies for the drinking water industry . While previous UV disinfection studies have focused on the inactivation of Cryptosporidium and Giardia as well as viruses and common bacteria, this research reports the ability of low- and medium pressure UV light to inactivate > 3.6 log10 of microsporidia Encephalitozoon intestinalis spores at a dose of 6 mJ/cm2 or higher as determined using a cell culture approach. Water Res, 2002 Jul, 36(12), 3141 - 9 Development of a Ct equation for the inactivation of Cryptosporidium oocysts with ozone; Clark RM et al.; Cryptosporidium parvum, a protozoan parasite, has been implicated in a number of waterborne disease outbreaks . It is difficult to inactivate using free chlorine, but appears to be easily inactivated by ozone . Therefore, the US EPA has promulgated the Interim Enhanced Surface Water Treatment Rule, which for the first time, addresses the control of C . parvum in drinking water . The use of Ct (concentration of disinfectant in mg/L times, time in minutes) values is being considered as one of the options for controlling this organism . This paper proposes a Ct equation, based on first order kinetics, to provide guidance to drinking water utilities for the application of ozone for controlling C parvum oocysts in drinking water . The equation, which provides mean estimates of inactivation, was developed using standard statistical techniques and currently available field and bench scale data . In addition, the possibility of using a statistically conservative upper bound Ct value in order to insure an appropriate safety factor is explored. Water Res, 2002 Jul, 36(12), 3045 - 53 Molecular size fractions of treated aquatic humus; Myllykangas T et al.; The effects of ozone, chlorine, hydrogen peroxide, and permanganate on the aquatic humic matter with different molecular size fractions and the organic acid formation in drinking water treatment were studied . Aquatic humus in lake water (LW), artificially recharged groundwater (AW), and purified artificially recharged groundwater (PW) were fractionated by high-pressure size-exclusion chromatography (HP-SEC) with UV-254nm detection before and after oxidation, a technique which resulted generally in seven peaks . The sum of the molecular size fractions (SMSF) of the LW was reduced by 47% during the bank filtration process, and the SMSF of the AW was reduced by 55% during the process in the water treatment plant . The oxidation of the AW resulted in reductions in the range of 18-35% of the SMSF; the respective range of the PW was 15-69% . However, the content of the total organic carbon (TOC) reduced only slightly, and a high correlation between the TOC and the SMSF (0.911) was observed in the whole material . The greatest decreases appeared in the highest-molecular-weight fractions while the low-molecular-weight fractions remained nearly unchanged . The total content of the six organic small-molecular-weight acids (sum of the organic acids, SOA) (formate, acetate, propionate, pyruvate, oxalate, and citrate) varied between 0.1-5.1% and 0.1-9.7% of the reduced TOC in the AW and the PW, respectively . The formation of the SOA, especially of oxalate, was the greatest after hydrogen peroxide combined with ozonation (as much as 1,100 microg/L), while chlorination resulted in the SOA of < 50 microg/L. Water Res, 2002 Jul, 36(12), 2959 - 66 The mineralisation of methamidophos using ionised AN air water treatment pilot system and ultraviolet irradiation; Hung DQ et al.; The degradation of methamidophos in pure bi-distilled water (with initial concentration 5 mg L(-1), pH = 7 at the beginning) was studied . For the first time, 2 ionised air water treatment pilot systems (IAPS-1, -2, no additives) were set up (Figs . 1 and 2) . The degradation of methamidophos was carried out with only the IAPS-2 and with two different ultraviolet (UV) lamps: high- and low-pressure mercury lamps (HP and LP, with or without H202) . The kinetics of these oxidative processes was investigated . The highest rate constant was reached by the LP, 0.2% H2O2 (k = 0.7524min(-1), t 1/2 = 0.9min), followed closely by both treatments with the HP, 0.2% H202 (k = 0.6328min(-1), t 1/2 = 1.1 min) and LP, 0% H202 (k = 0.4749min(-1), t 1/2 = 1.5 min) . The lowest rate constants were achieved from the HP, 0% H202 process (k = 0.0303min(-1), t 1/2 = 22.9min) and from the IAPS-2 (k = 0.0117 min(-1), t 1/2 = 59.2min) . The mineralisation of methamidophos was confirmed by the determination of the anions produced including NO3-, PO4(3-) and SO4(2-) during and at the end of each experiment . The toxicity of water samples before, during and after the experiments was tested with Daphnia test. Tissue Cell, 2002 Apr, 34(2), 129 - 33 Immunohistochemical localization of three different immunoglobulin classes in the Harderian gland of young chickens; Ohshima K et al.; The chicken Harderian gland (HG) was investigated using single immunohistochemical staining for one of the three different immunoglobulins (Igs) followed by Alcian blue/periodic acid Schiff (AB/PAS) staining and triple immunohistochemical staining for all of the Igs with hot water treatment . In the HG of 5-week-old chickens, IgG-containing plasma cells were more frequent than IgA- and IgM-containing cells . These numerous IgG-containing cells were predominantly accumulated in the central region of the stroma, whereas a small number of IgA- and IgM-containing cells were scattered in the peripheral region of the stroma . Also, the plasma cells containing PAS-positive Russell bodies (RBs) exhibited distinct immunoreactivity for one of the Igs, being inversely proportional to the intensity of PAS reaction . The RB-containing cells positive for IgA were more frequent than those positive for IgM, whereas those positive for IgG were very rare . The most distinct feature of the IgG-containing plasma cells was a PAS-positive globule located close to the nucleus . Triple immunostaining with hot water treatment simultaneously identified these three Igs in normal plasma cells and RB-containing ones in the stroma of the chicken HG. Environ Technol, 2002 Jul, 23(7), 813 - 21 Application of a dynamic 2-D mixing model to assess the impact of chemical |