More Papers

Formation of an F' Plasmid by Recombination between Imperfectly Repeated Chromosomal Rep Sequences: a Closer Look at an Old Friend (F'₁₂₈ pro lac).
Eric Kofoid, 2003.Plasmid F'₁₂₈ was formed by an exchange between chromosomal Rep sequences that placed lac near dinB between many pairs of Rep sequences . Plasmid F'₁₂₈ is critical for selection-enhanced lac reversion (adaptive mutation), which requires prior lac amplification . The structure of F'₁₂₈ supports the idea that amplification is initiated by Rep-Rep recombination and that general mutagenesis requires coamplification of dinB (error-prone polymerase) with lac .

Bacteriophage SPP1 Chu Is an Alkaline Exonuclease in the SynExo Family of Viral Two-Component Recombinases.
Trina S. Vellani, 2003.Many DNA viruses concatemerize their genomes as a prerequisite to packaging into capsids . Concatemerization arises from either replication or homologous recombination . Replication is already the target of many antiviral drugs, and viral recombinases are an attractive target for drug design, particularly for combination therapy with replication inhibitors, due to their important supporting role in viral growth . To dissect the molecular mechanisms of viral recombination, we and others previously identified a family of viral nucleases that comprise one component of a conserved, two-component viral recombination system . The nuclease component is related to the exonuclease of phage

and is common to viruses with linear double-stranded DNA genomes . To test the idea that these viruses have a common strategy for recombination and genome concatemerization, we isolated the previously uncharacterized 34.1 gene from Bacillus subtilis phage SPP1, expressed it in Escherichia coli, purified the protein, and determined its enzymatic properties . Like

exonuclease, Chu (the product of 34.1) forms an oligomer, is a processive alkaline exonuclease that digests linear double-stranded DNA in a Mg²⁺-dependent reaction, and shows a preference for 5'-phosphorylated DNA ends . A model for viral recombination, based on the phage

Red recombination system, is proposed .

What Is Genetics?, What Is Functional Genomics?, What Is Botulism?, What Is Nitrification?, What Is Genetic Engineering?, r, Microbiology, c, Microbe, n, Bacterium, n, Microorganisms, e, Microbes, a, S. cerevisiae, c, Bacillus subtilis, e, Bacteroides, o, Salmonella typhimurium, n, Antibiotics, e, Escherichia coli, r, Biological reactors

Scientific Publications - Work Done by Microbiology Reader Bioscreen C
Agricultural Microbiology Anaerobic Microbiology Antimicrobial Susceptibility Artificial Atmosphere Bioassay of Antibiotics Biofilm Microbiology Bioreactor Technology Biotechnology Cell Biology Clinical Microbiology Environmental Microbiology Experiments with Yeast	Fermentation Food Microbiology Functional Genomics Gene Technology Growth Media Development Growth Rate and Lag Time Industrial Microbiology Medical/Pharmaceutical Field Microbiological Assay Microbiological Research Microbiology of Cosmetics go to a specific theme...	Military Microbiology Molecular Microbiology Mutagenicity and Genotoxicity Oral Microbiology Patents Postantibiotic Studies Soil Microbiology Spore Microbiology Veterinary Microbiology Waste/Wastewater Treatment Water Microbiology Wine Microbiology

© 2005 Transgalactic Ltd (manufacturer of Bioscreen C software) | Privacy Statement | P.O. Box 1393, 00101 Helsinki, Finland, phone: +358 9 85172920, fax: +358 9 8749481, e-mail: microbiology@bionewsonline.com

Last modified: May 25, 2005