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Characterization of Nocardia asteroides Isolates from Different Ecological Habitats on the Basis of Repetitive Extragenic Palindromic-PCR Fingerprinting. Hideki Yamamura, 2004.Thirteen isolates of Nocardia asteroides from both soils and aquatic samples (lake and moat sediments, as well as scum from activated sludge), together with a type strain and two known clinical isolates of this species, were characterized by repetitive extragenic palindromic-PCR fingerprinting with the BOX-A1R primer . The resulting DNA fingerprint patterns proved to be strain specific, and cluster analysis distinguished the soil isolates, the aquatic isolates, and the known strains as being in separate groups . Molecular Evolution of the Intimin Gene in O111 Clones of Pathogenic Escherichia coli. Cheryl L. Tarr, 2002.Intimin is an important virulence factor in two groups of enteric pathogens: enteropathogenic Escherichia coli (EPEC), which is a major cause of infant diarrhea in the developing world, and enterohemorrhagic E . coli (EHEC), which has caused large food-borne outbreaks of hemorrhagic colitis in the United States and other developed countries . Intimin is encoded on a 35-kb pathogenicity island called the locus of enterocyte effacement (LEE) . At least five antigenic types have been described for the highly variable gene, and each type is generally characteristic of particular evolutionary lineages . We determined the nucleotide sequences of intimin and other LEE genes in two O111 clones that have not been amenable to typing . The sequences from both O111:H8 and O111:H9 differed from the Int-ß that is typical of other clones in the same evolutionary lineage . The sequence from the O111:H8 strains was a mosaic of divergent segments that alternately clustered with Int-  , Int-ß, or Int- . The sequence from the O111:H9 clone consistently showed a close relationship with that from E2348/69, a distantly related strain that expresses Int- . The results suggest that there have been multiple acquisitions of the LEE in the EHEC 2/EPEC 2 clonal lineage, with a recent turnover in either O111:H8 or its close relatives . Amino acid substitutions that alter residue charge occurred more frequently than would be expected under random substitution in the extracellular domains of intimin, suggesting that diversifying selection has promoted divergence in this region of the protein . An N-terminal domain that presumably functions in the periplasm may also be under positive selection .
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