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Three Different Systems Participate in L-Cystine Uptake in Bacillus subtilis. Pierre Burguière, 2004.The symporter YhcL and two ATP binding cassette transporters, YtmJKLMN and YckKJI, were shown to mediate L-cystine uptake in Bacillus subtilis . A triple  yhcL
ytmJKLMN
yckK
mutant was unable to grow in the presence of L-cystine
and to take up L-cystine . We propose that
yhcL, ytmJKLMN, and yckKJI should be renamed
tcyP, tcyJKLMN, and tcyABC, respectively . The
L-cystine uptake by YhcL (Km = 0.6 µM) was
strongly inhibited by seleno-DL-cystine, while
the transport due to the YtmJKLMN system (Km = 2.5 µM)
also drastically decreased in the presence of DL-cystathionine,
L-djenkolic acid, or S-methyl-L-cysteine .
Accordingly, a
ytmJKLMN
mutant did not grow in the presence of 100 µM DL-cystathionine,
100 µM L-djenkolic acid, or 100 µM S-methyl-L-cysteine .
The expression of the ytmI operon and the yhcL gene was
regulated in response to sulfur availability, while the level of
expression of the yckK gene remained low under all the
conditions tested .
Molecular Characterization of a Carbapenem-Hydrolyzing Class A ß-Lactamase, SFC-1, from Serratia fonticola UTAD54. Isabel Henriques, 2004.An environmental isolate of Serratia fonticola resistant to carbapenems contains a gene encoding a class A ß-lactamase with carbapenemase activity . The enzyme was designated SFC-1 . The blaSFC-I gene is contained in the chromosome of S . fonticola UTAD54 and is absent from other S . fonticola strains .
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