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Osmoregulation of Dimer Resolution at the Plasmid pJHCMW1 mwr Locus by Escherichia coli XerCD Recombination. Huong Pham, 2002.Xer-mediated dimer resolution at the mwr site of plasmid pJHCMW1 is osmoregulated in Escherichia coli . Whereas under low-salt conditions, the site-specific recombination reaction is efficient, under high-salt conditions, it proceeds inefficiently . Regulation of dimer resolution is independent of H-NS and is mediated by changes in osmolarity rather than ionic effects . The low level of recombination at high salt concentrations can be overcome by high levels of PepA or by mutating the ARG box to a sequence closer to the E . coli ARG box consensus . The central region of the mwr core recombination site plays a role in regulation of site-specific recombination by the osmotic pressure of the medium .
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