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tmRNA in Caulobacter crescentus Is Cell Cycle Regulated by Temporally Controlled Transcription and RNA Degradation. Kenneth C. Keiler, 2003.SsrA, or tmRNA, is a small RNA found in all bacteria that intervenes in selected translation reactions to target the nascent polypeptide for rapid proteolysis . We have found that the abundance of SsrA RNA in Caulobacter crescentus is regulated with respect to the cell cycle . SsrA RNA abundance increases in late G1 phase, peaks during the G1-S transition, and declines in early S phase, in keeping with the reported role for SsrA in the timing of DNA replication initiation . Cell cycle regulation of SsrA RNA is accomplished by a combination of temporally controlled transcription and regulated RNA degradation . Transcription from the ssrA promoter peaks late in G1, just before the peak in SsrA RNA abundance . SsrA RNA is stable in G1-phase cells and late S-phase cells but is degraded with a half-life of 4 to 5 min at the onset of S phase . This degradation is surprising, since SsrA RNA is both highly structured and highly abundant . This is the first observation of a structural RNA that is cell cycle regulated .
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