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Verocytotoxin-Producing Escherichia coli in Wild Birds and Rodents in Close Proximity to Farms. Eva Møller Nielsen, 2004. Differential Expression and Localization of Mn and Fe Superoxide Dismutases in the Heterocystous Cyanobacterium Anabaena sp . Strain PCC 7120. Tao Li, 2002.Superoxide dismutases (Sods) play very important roles in preventing oxidative damages in aerobic organisms . The nitrogen-fixing heterocystous cyanobacterium Anabaena sp . strain PCC 7120 has two Sod-encoding genes: a sodB, encoding a soluble iron-containing Sod (FeSod), and a sodA, encoding a manganese-containing Sod (MnSod) . The FeSod was purified and characterized . A recombinant FeSod was also obtained by overproduction in Escherichia coli. Immunoblot study of the FeSod during induction of heterocyst differentiation showed that the cells produced six- to eightfold more FeSod 8 h after a shift from a nitrogen-replete condition to a nitrogen-depleted condition . However, the amount of FeSod protein in filaments with mature heterocysts was the same as that in filaments grown with combined nitrogen . Superoxide anion-generating chemicals such as methyl viologen did not induce upregulation of the sodB gene expression . The predicted preprotein of the sodA gene has a leader peptide and a motif for membrane attachment at the N terminus of the mature protein . Activity staining after gel electrophoresis of the purified thylakoid membranes showed that most of the MnSod in Anabaena sp . strain PCC 7120 was located on thylakoids toward the lumenal side . Expression of the sodA gene in E . coli shows that the leader peptide was required for its activity and the membrane localization of the MnSod . Northern hybridization detected one 0.82-kb transcript of sodA . The sodA gene was upregulated by methyl viologen, whereas its amount was unchanged during heterocyst differentiation . Immunoblotting and activity staining showed that isolated heterocysts contained a lower but still significant amount of FeSod, suggesting that its function is required in heterocysts . No MnSod was observed in isolated heterocysts . These results show that the two different Sod proteins have differentiated roles in Anabaena sp . strain PCC 7120 .
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