S. A. Kohlhoff 2004 1885

Antimicrobial Agents and Chemotherapy, May 2004, p . 1885-1886, Vol . 48, No . 5

In Vitro Activity of a Novel Diaminopyrimidine Compound, Iclaprim, against Chlamydia trachomatis and C . pneumoniae

S . A . Kohlhoff,¹^* P . M . Roblin,¹ T . Reznik,¹ S . Hawser,² K . Islam,² and M . R . Hammerschlag¹

Department of Pediatrics, Downstate Medical Center, State University of New York, Brooklyn, New York,¹ Arpida, Ltd., Münchenstein, Switzerland²

Received 19 November 2003/ Returned for modification 24 December 2003/ Accepted 29 January 2004

ABSTRACT

The in vitro activities of iclaprim, a novel dihydrofolate reductase inhibitor, azithromycin, and levofloxacin were tested against 10 strains of Chlamydia trachomatis and 10 isolates of Chlamydia pneumoniae . For C . trachomatis and C . pneumoniae, the iclaprim MIC and minimal bactericidal concentration at which 90% of isolates were inhibited (MIC₉₀ and MBC₉₀) were 0.5 µg/ml, compared to an azithromycin MIC₉₀ and MBC₉₀ of 0.125 µg/ml and levofloxacin MIC₉₀s and MBC₉₀s of 1 µg/ml for C . trachomatis and 0.5 µg/ml for C . pneumoniae .

TEXT

Chlamydia trachomatis infection is the most common sexually transmitted infection in the United States, causing more than 3 million cases of cervicitis and urethritis every year . Chlamydia pneumoniae is a frequent cause of community-acquired respiratory infections, including pneumonia and bronchitis, in adults and children . Dihydrofolate reductase (DHFR) inhibitors, specifically trimethoprim, have been used for many years to treat infections due to a wide range of bacteria, usually in combination with sulfonamides . The antimicrobial activity against bacterial pathogens is mediated through inhibition of thymidylate synthesis and therefore nucleic acid synthesis . Since mammalian cells do not synthesize folic acid, human purine synthesis is not affected significantly . Even though chlamydiae synthesize folate, trimethoprim (TMP) or TMP-sulfamethoxazole cannot be used for the treatment of chlamydial disease, because trimethoprim is not active against chlamydiae (1) .

Iclaprim (formerly AR-100), a novel DHFR inhibitor, has potent activity against gram-positive and gram-negative bacteria, including Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Haemophilus influenzae, and Escherichia coli (C . G . Gemmell and G . Middlemas, Abstr . 42nd Intersci . Conf . Antimicrob . Agents Chemother., abstr . F-2022, 2002; C . E . Good, A . Windau, S . Bajaksouzian, M . R . Jacobs, and P . C . Appelbaum, Abstr . 42nd Intersci . Conf . Antimicrob . Agents Chemother., abstr . F-2023, 2002; R . L . Then, Abstr . 42nd Intersci . Conf . Antimicrob . Agents Chemother., abstr . F-2021, 2002; M . R . Jacobs, A . Windau, S . Bajaksouzian, and P . C . Appelbaum, Abstr . 42nd Intersci . Conf . Antimicrob . Agents Chemother., abstr . F-2026, 2002; A . Windau, S . Bajaksouzian, P . C . Appelbaum, and M . R . Jacobs, Abstr . 42nd Intersci . Conf . Antimicrob . Agents Chemother., abstr . F-2025, 2002) . There are no data on the activity of iclaprim against Chlamydia spp.; however, trimethoprim has previously been reported to have no significant activity against C . trachomatis in vitro (1) . Therefore, we compared the activity of iclaprim, a new DHFR inhibitor, with the activities of azithromycin and levofloxacin against C . trachomatis and C . pneumoniae.

We tested 10 strains of C . trachomatis, including four clinical endocervical isolates: N16/CX, N17/CX, N18/CX, N19/CX, and ATCC strains HUW-43/CX (VR-879), JUW-36/CX (VR-886), IUW-12/UR (VR-880), LGV 434 (VR-902B), FIC-CAL3/CX (VR-346), and E-BOUR (VR-384B) . Isolates of C . pneumoniae tested included three reference isolates, TW183 (VR-2282), CM-1 (VR-1360), and AR39 (ATCC 53592), and seven clinical isolates from adults and children with pneumonia, W6805, T2023 (ATCC VR-1310), T2043 (ATCC VR-1355), BAL-15, BAL-16, BAY255, and BAY493 .

Antimicrobial agents were supplied as powders and solubilized according to manufacturers' instructions . Iclaprim (Arpida, Basel, Switzerland), azithromycin (Pfizer, New York, N.Y.), and levofloxacin (Ortho Pharmaceuticals, Raritan, N.J.) were used . In addition, TMP (Roche, N.J.) was tested against two isolates of C . pneumoniae (TW183 and CM-1) . Susceptibility testing of C . pneumoniae was performed in cell culture by using HEp-2 cells grown in 96-well microtiter plates as previously described (3) . Each experiment was set up in duplicate plates . Each well was inoculated with 0.1 ml of the test organism diluted to yield 10³ to 10⁴ inclusion-forming units per ml, centrifuged at 1,700 x g for 1 h, and incubated at 35°C for 1 h . Wells were then aspirated and overlaid with 0.2 ml of medium containing 1 µg of cycloheximide per ml and serial twofold dilutions of the test drug . After incubation at 35°C for 72 h, the cultures in one plate were fixed and stained for inclusions with fluorescein-conjugated antibody to the lipopolysaccharide genus antigen (Pathfinder; Bio-Rad Labs, Hercules, Calif.) . The MIC was the lowest antibiotic concentration at which no inclusions were seen . The minimal bactericidal concentration (MBC) was determined by aspirating the antibiotic-containing medium of the second plate, washing wells twice with phosphate-buffered saline, and adding antibiotic-free medium . Cultures were frozen at –70°C, thawed, passed onto fresh new cells, incubated for 72 h, and then fixed and stained as described above . The MBC was the lowest antibiotic concentration that resulted in no inclusions after passage . All assays were performed in triplicate .

The MICs and MBCs for C . trachomatis and C . pneumoniae are shown in Tables 1 and 2 . The MICs and MBCs at which 90% of isolates were inhibited (MIC₉₀s and MBC₉₀s) of iclaprim for C . trachomatis and C . pneumoniae were 0.5 µg/ml . The MIC₉₀s and MBC₉₀s of azithromycin and levofloxacin for C . trachomatis were 0.125 and 1 µg/ml, respectively . The MIC₉₀s and MBC₉₀s of azithromycin and levofloxacin for C . pneumoniae were 0.125 and 0.5 µg/ml, respectively . The MICs and MBCs of iclaprim for C . trachomatis and C . pneumoniae indicate that its activity is comparable to those of both azithromycin and levofloxacin . By contrast to the activity of iclaprim, trimethoprim was inactive . The MICs and MBCs of trimethoprim for C . pneumoniae TW183 and CM-1 were 128 µg/ml .

TABLE 1 . Activity of iclaprim and other antibiotics against 10 strains of C . trachomatis

TABLE 2 . Activity of iclaprim and other antibiotics against 10 isolates of C . pneumoniae

Iclaprim is a bactericidal drug that demonstrated activity comparable to those of both azithromycin and levofloxacin . MICs and MBCs were consistent for all strains and isolates tested, especially in view of their wide geographic distribution . Even though both trimethoprim and iclaprim target DHFR, iclaprim was much more active . Trimethoprim had poor activity against C . pneumoniae in this study, similar to what was previously reported for C . trachomatis, with MICs of 128 µg/ml (2) . Whereas most bacteria are unable to utilize exogenous folate, Fan et al . demonstrated that C . trachomatis L₂, C . psittaci 6BC, and C . psittaci strain francis appeared to both synthesize and to transport folate from the host cell in various degrees, depending on the strain (1) . The relevance of this folate transport mechanism for in vitro or in vivo susceptibilities to trimethoprim and sulfonamides is not clear . Sulfonamides block the bacterial folic acid metabolism at a different site and have been used in a fixed combination with trimethoprim for synergistic effect . Iclaprim was synergistic when tested in combination with sulfonamides against a wide range of gram-positive and gram-negative bacteria (S . Hawser, L . Weiss, M . Fischer, D . Gillessen, I . Kompis, and K . Islam, Abstr . 42nd Intersci . Conf . Antimicrob . Agents Chemother., abstr . F-2019, 2002) . Although C . trachomatis is susceptible to sulfonamides, C . pneumoniae is not (1) . Earlier studies of the synergy of trimethoprim and sulfamethoxazole against C . trachomatis found the combined activity to be only additive, with most of the activity being due to the sulfonamide, but it may be useful to perform synergy studies with iclaprim and sulfonamides to look for similar effects on C . trachomatis (2) . However, based on its in vitro activity in this study, using iclaprim as a single drug for the treatment of chlamydial infections could be adequate .

These data suggest that new DHFR inhibitors may have a potential role in the treatment of respiratory infections due to C . pneumoniae as well as genital infections caused by C . trachomatis . Studies to evaluate efficacy in patients using culture-based diagnostic methods are indicated .

FOOTNOTES

* Corresponding author . Mailing address: Department of Pediatrics, Box 49, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203-2098 . Phone: (718) 270-7588 . Fax: (718) 270-3210 . E-mail: stephan.kohlhoff{at}downstate.edu .

REFERENCES

Fan, H., R . C . Brunham, and G . McClarty. 1992 . Acquisition and synthesis of folates by obligate intracellular bacteria of the genus Chlamydia. J . Clin . Investig . 90:1803-1811.
Hammerschlag, M . R. 1982 . Activity of trimethoprim-sulfamethoxazole against Chlamydia trachomatis in vitro . Rev . Infect . Dis . 4:500-504.
Roblin, P . M., T . Reznik, and M . R . Hammerschlag. 2002 . In vitro activity of garenoxacin against recent clinical isolates of Chlamydia pneumoniae. Int . J . Antimicrob . Agents 21:578-580.

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