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Methylotrophy in Methylobacterium extorquens AM1 from a Genomic Point of View. Ludmila Chistoserdova, 2003. Strategy for Cloning Large Gene Assemblages as Illustrated Using the Phenylacetate and Polyhydroxyalkanoate Gene Clusters. Belén García, 2004. Regulation of the Bacillus subtilis Extracytoplasmic Function Protein  Y and Its Target Promoters.Min Cao, 2003.The Bacillus subtilis extracytoplasmic function sigma factor Y is of unknown function . We demonstrate that the sigY operon is expressed from an autoregulatory promoter site, PY . We selected for transposon-induced mutations that upregulate PY transcription in an attempt to identify genes involved in
Y regulation . The resulting insertions disrupted yxlC, the gene immediately downstream of sigY . However, the phenotype of the yxlC::Tn10 insertion was due to polarity on the downstream genes of the sigY operon; a nonpolar insertion in yxlC did not lead to derepression of PY . Further analyses revealed that both yxlD and yxlE encoded proteins important for the negative regulation of
Y activity . A comparison of the transcriptomes of wild-type and yxlC::Tn10 mutant strains revealed elevated expression of several operons . However, only one additional gene, ybgB, was unambiguously identified as a direct target for
Y . This was supported by analysis of direct targets for
Y transcription with whole-genome runoff transcription followed by macroarray analysis .
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