Text

Biological Effects of Trichoderma harzianum Peptaibols on Mammalian Cells.
Joanna Peltola, 2004.Trichoderma species isolated from water-damaged buildings were screened for toxicity by using boar sperm cells as indicator cells . The crude methanolic cell extract from Trichoderma harzianum strain ES39 inhibited the boar sperm cell motility at a low exposure concentration (50% effective concentration, 1 to 5 µg [dry weight] ml of extended boar semen^–1) . The same exposure concentration depleted the boar sperm cells of NADH₂ . Inspection of the exposed boar sperm cells by transmission electron microscopy revealed damage to the plasma membrane . By using the black lipid membrane technique, it was shown that the semipurified metabolites (eluted from a SepPak C₁₈ cartridge) of T . harzianum strain ES39 induced voltage-dependent conductivity . The high-performance liquid chromatography-purified metabolites of T . harzianum strain ES39 dissipated the mitochondrial membrane potential (

_m) of human lung epithelial carcinoma cells (cell line A549) . The semipurified metabolites (eluted from a SepPak C₁₈ cartridge) of T . harzianum strain ES39 were analyzed by mass spectrometry (MS) . Matrix-assisted laser desorption ionization and nanoflow electrospray ionization MS revealed five major peptaibols, each of which contained 18 residues and had a mass ranging from 1,719 to 1,775 Da . Their partial amino acid sequences were determined by collision-induced dissociation tandem MS .

Speed versus Efficiency in Microbial Growth and the Role of Parallel Pathways.
Robert B. Helling, 2002.Many microorganisms have sets of parallel pathways for ATP production in respiration and for ATP utilization in glutamate synthesis . The alternatives differ in efficiency of ATP production and utilization . The choice among these parallel pathways has been hypothesized to control the speed and efficiency of growth . Thus, the organism should be able to alleviate (or exaggerate) deficiency in one pathway by deleting another . I show here that in Escherichia coli the effect of lack of the glutamate-synthesizing enzyme glutamate dehydrogenase on glucose-limited growth is altered predictably by ndh, cyo, and cyd mutations affecting parallel pathways leading to ATP synthesis in respiration .

What Is Bioengineering?, What Is Biofilter?, What Is Cell Biology?, What Is Yeast?, What Is Water Purification?, i, Bacteriology, i, Microbe, n, Microbes, s, Bacteria, a, Bacterium, n, Microbial, s, Vibriosis, s, Streptococci, e, Antibiotic treatment, n, Gram negative, a, Antibiotics, o, Bacteriological

Scientific Publications - Work Done by Microbiology Reader Bioscreen C
Agricultural Microbiology Anaerobic Microbiology Antimicrobial Susceptibility Artificial Atmosphere Bioassay of Antibiotics Biofilm Microbiology Bioreactor Technology Biotechnology Cell Biology Clinical Microbiology Environmental Microbiology Experiments with Yeast	Fermentation Food Microbiology Functional Genomics Gene Technology Growth Media Development Growth Rate and Lag Time Industrial Microbiology Medical/Pharmaceutical Field Microbiological Assay Microbiological Research Microbiology of Cosmetics go to a specific theme...	Military Microbiology Molecular Microbiology Mutagenicity and Genotoxicity Oral Microbiology Patents Postantibiotic Studies Soil Microbiology Spore Microbiology Veterinary Microbiology Waste/Wastewater Treatment Water Microbiology Wine Microbiology

© 2005 Transgalactic Ltd (manufacturer of Bioscreen C software) | Privacy Statement | P.O. Box 1393, 00101 Helsinki, Finland, phone: +358 9 85172920, fax: +358 9 8749481, e-mail: microbiology@bionewsonline.com

Last modified: May 25, 2005