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In Vitro Susceptibilities of Mycoplasma hyopneumoniae Field Isolates. J. Vicca, 2004.The in vitro susceptibilities of 21 Mycoplasma hyopneumoniae field isolates were determined using a broth microdilution technique . One isolate showed acquired resistance to lincomycin, tilmicosin, and tylosin, while five isolates were resistant to flumequine and enrofloxacin . Acquired resistance against these antimicrobials in M . hyopneumoniae field isolates was not reported previously . Lack of Strand Bias in UV-Induced Mutagenesis in Escherichia coli. Damian Gawel, 2002.We have investigated whether UV-induced mutations are created with equal efficiency on the leading and lagging strands of DNA replication . We employed an assay system that permits measurement of mutagenesis in the lacZ gene in pairs of near-identical strains . Within each pair, the strains differ only in the orientation of the lacZ gene with respect to the origin of DNA replication . Depending on this orientation, any lacZ target sequence will be replicated in one orientation as a leading strand and as a lagging strand in the other orientation . In contrast to previous results obtained for mutations resulting from spontaneous replication errors or mutations resulting from the spontaneous SOS mutator effect, measurements of UV-induced mutagenesis in uvrA strains fail to show significant differences between the two target orientations . These data suggest that SOS-mediated mutagenic translesion synthesis on the Escherichia coli chromosome may occur with equal or similar probability on leading and lagging strands .
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