Microbiology Reader Bioscreen C and Software Research Express - Microbiological Growth Curves

In batch culturing a growth of micro-organisms can be divided into 4 main phases: lag time (no growth), exponential growth phase, stationary phase and death phase.

All microorganisms produce a turbidity during growth in a liquid growth medium. This turbidity development is measured automatically by an incubating microbiology reader Bioscreen C.

200 growth curves are produced simultaneously - each well gives one growth curve (Figure 1). Growth curves of almost any strain and broth are produced directly into MS Excel. Growth curves are displayed by using a logarithmic or linear scaling - for some kind of work it is better to use a logarithmic OD axis and for some kind of work it is better to use a linear OD axis.

Bioscreen C growth curves (turbidity curves) are shown as a point-to-point graph (connected turbidity values). These kind of point-to-point turbidity curves are enough accurate for a wide range of microbiology work.

A turbidity graph of original OD values shows a combined turbidity of growth medium and a microorganism culture. By software Research Express a turbidity component of growth medium can be removed from a graph and as a result a turbidity of microbiological culture only is displaid. If a start concentration of microorganisms is very low then a point-to-point turbidity graph does not show precisely a microorganism (biomass) growth for a very start of a growth curve (i.e. at time when a turbidity of biomass is below a turbidity of growth medium). In these studies where a very start part of growth curve is wanted to display in a more precise way it is needed to use as transparent growth medium as possible or alternatively a whole turbidity graph or any part of a turbidity graph can be put into a mathematical formula (mathematical formulas for such justification work are not included in a software Research Express; these can be found in a microbiology literature).

Use of mathematical formulas in software Research Express is supported by a special sheet ODc (OD-calculated) in an experiment workbook and by permitting a user to link user-made personalized template sheets (i.e. MS Excel sheets containing user formulas) to over 20 default MS Excel sheets (with default microbiological calculations and graph) of an experiment workbook.

Microbiological Growth Curves

Growth curves on below are generated by Microbiology Reader Bioscreen C and software Research Express

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Larger collection of growth curves screens...

Software Research Express Screens

Software Research Express controls a Microbiology Reader Bioscreen C, generates growth curves and performs microbiological calculations.

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Larger collection of software Research Express screens...

© 2005 Transgalactic Ltd (manufacturer of Bioscreen C software) | Privacy Statement | P.O. Box 1393, 00101 Helsinki, Finland, phone: +358 9 85172920, fax: +358 9 8749481, e-mail: microbiology@bionewsonline.com
 

Last modified: May 25, 2005