Scientific
Publications - Work Done by Microbiology Reader Bioscreen C
Yasuko Jojima, Yasuhiro Mihara, Sonoko Suzuki, Kenzo Yokozeki, Shigeru Yamanaka
and Ryosuke Fudou,
Saccharibacter floricola gen. nov., sp. nov., a novel osmophilic
acetic acid bacterium isolated from pollen,
Int J Syst Evol Microbiol 54 (2004), 2263-2267
ABSTRACT
Three Gram-negative, aerobic, rod-shaped bacterial strains were isolated, from
the pollen of Japanese flowers, as producers of xylitol; these strains were
subjected to a polyphasic taxonomic study. Phylogenetic analyses of the 16S rRNA
gene sequences demonstrated that these three isolates formed a new cluster
within a group of acetic acid bacteria in the -Proteobacteria. The
characteristics of the three isolates were as follows: (i) their predominant
quinone was Q-10; (ii) their cellular fatty acid profile contained major amounts
of 2-hydroxy acids and an unsaturated straight-chain acid (C18 : 1ω7c);
and (iii) their DNA G+C contents were in the range 51·9–52·3 mol%, which is
around the lower limit of the reported range for the genera of acetic acid
bacteria. The negligible or very weak productivity of acetic acid from ethanol
and the osmophilic growth properties distinguished these strains from other
acetic acid bacteria. The unique phylogenetic and phenotypic characteristics
suggest that the three isolates should be classified within a novel genus and
species with the proposed name Saccharibacter floricola gen. nov., sp. nov. The
type strain is strain S-877T (=AJ 13480T=JCM 12116T=DSM
15669T).
(Abstract online)
