Scientific Publications - Work Done by Microbiology Reader Bioscreen C

Wejdemar, K., Vitamins that stimulate the growth of Butyrivibrio fibrisolvens strains grown in rumen fluid, (Abstract), Swedish University of Agricultural Sciences, Department of Animal Nutrition and Management, Kungsängen Research Station; report, 1 pp

ABSTRACT

Butyrivibrio fibrisolvens strains grow poorly in rumen fluid supplemented with glucose unless yeast extract is added (1). Experiments had shown that B. fibrisolvens strain TC33 is stimulated when the vitamins pyroxine and folic acid are added to the rumen fluid media (paper in preparation). New experiments were conducted to examine which vitamins stimulated growth of other B. fibrisolvens strains. Eight strains representing different biotypes of B. fibrisolvens isolated by van Gylswyk (2) were selected with the aid of Principal Component Analysis (Sirius, Pattern Recognition Systems A/S, Norway) with VFA production in 40 % rumen fluid medium as criterion. The strains were cultivated in rumen fluid supplemented with glucose, essentially vitamin-free casein hydrolysate, bicarbonate and reducing agent (cysteine and sulphide). Rumen fluid was collected three hours after the morning feed from three lactating cows that were fed a mixture of grass silage (65 % DM) and concentrate (35 % DM) for four weeks before rumen fluid was collected. The rumen fluid was supplemented singly with 10 different vitamins or groups of vitamins (mostly B-vitamins). Five replicates were used for each treatment. An automated culturing apparatus (Bioscreen, Labsystems Oy, Helsinki, Finland)

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Last modified: May 25, 2005