Scientific Publications - Work Done by Microbiology Reader Bioscreen C

Tero Eerikäinen, Erkka Meskanen, and Veera Home, Modelling of bacterial bioreactor cultivation, “Proceedings of the 1st International Congress on Bioreactor Technology In Cell, Tissue Culture and Biomedical Applications” Tampmere, Finland, 14-18 July 2003, pp. 137-147

ABSTRACT

Modelling of bioreactor cultivation can be utilized different ways depending on the purpose the models are needed for. Process development, raw material cost, production rate and down stream processing can be modelled with proper tools in a straightforward manner. Examples are taken from bacterial cultivations for exopolysaccharide production. Different tools for modelling are shown in this methodological representation including screening and optimisation of medium components and process economics calculation.

Economic evaluation of bioreactor cultivation and down stream processes can be carried out different ways. Here, a simulation environment, SuperPro Designer (Intelligen, Inc), was used in the development work, which includes process design and profitability analysis. Modelling and simulating the cultivation process with this tool helps one to find out the cost distribution between different process parts and focus on the process bottlenecks.

Bioreactor environmental variables like temperature and pH should be optimised when possible and a recommended approach is the use of an experimental design along with response surface modelling. This is the most efficient way to find optimal cultivation conditions and to minimize unnecessary experiments.

Raw materials costs and effect on the down stream process were the reasons that caused the demand for synthetic medium development. Incubations in the turbidometric microbiology reader Bioscreen C along with fractional factorial screening and central composite designs were used to optimisation of synthetic growth medium. Various ion concentrations were the model factors and specific growth rate was the main response.

(Abstract online)

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Last modified: May 25, 2005