Scientific Publications - Work Done by Microbiology Reader Bioscreen C

Susana Rodríguez-Navarro, Bertrand Llorente, María Teresa Rodríguez-Manzaneque, Anna Ramne, Genoveva Uber, Denis Marchesan, Bernard Dujon, Enrique Herrero, Per Sunnerhagen, José E. Pérez-Ortín, Functional analysis of yeast gene families involved in metabolism of vitamins B₁ and B₆. Functional Analysis Report, Yeast, Volume 19, Issue 14 , Pages 1261-1276

ABSTRACT

In order to clarify their physiological functions, we have undertaken a characterization of the three-membered gene families SNZ1-3 and SNO1-3. In media lacking vitamin B₆, SNZ1 and SNO1 were both required for growth in certain conditions, but neither SNZ2, SNZ3, SNO2 nor SNO3 were required. Copies 2 and 3 of the gene products have, in spite of their extremely close sequence similarity, slightly different functions in the cell. We have also found that copies 2 and 3 are activated by the lack of thiamine and that the Snz proteins physically interact with the thiamine biosynthesis Thi5 protein family. Whereas copy 1 is required for conditions in which B₆ is essential for growth, copies 2 and 3 seem more related with B₁ biosynthesis during the exponential phase.

© 2005 Transgalactic Ltd (manufacturer of Bioscreen C software) | Privacy Statement | P.O. Box 1393, 00101 Helsinki, Finland, phone: +358 9 85172920, fax: +358 9 8749481, e-mail: microbiology@bionewsonline.com
 

Last modified: May 25, 2005