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Scientific
Publications - Work Done by Microbiology Reader
Skyttä, E. and Mattila-Sandholm, T., A quantitative method for assessing bacteriocins and other food antimicrobials by automated turbidometry, Journal of Microbiological Methods, 1991, vol. 14, pp. 77-88 ABSTRACT Automated turbidometry was used in assessing the antimicrobial
efficacy of bacteriocin-like inhibitors produced by Pedicoccus damnosus and
Pedicoccus pentasaceus. four bacterial strains, i.e., Pseudomonas aeruginosa,
Pseudomonas fragi, Salmonella enteritidis and Salmonella infantis were used as
the test organisms. The growth of the test organisms was kinetically monitored
throughout the incubation period and various growth curve parameters were used
as quantitative indicators of growth inhibition. The results obtained in this
study showed that a kinetic method based on automated turbidometry provides a
sophisticated tool for the determination of bacteriocin activity. A number of
growth curve parameters were applicable for the quantification of inhibitory
effects. Regression analysis showed that the linear relationship between the
level values and the log CFU counts was in most cases highly significant.
However, the area value after 24 h appeared to describe most effectively the
overall inhibitory effect since all the other growth curve parameters were
simple end-point values giving information solely at a single point of time.
Furthermore, the area reduction rate shown by the subsequent serial dilutions of
the antimicrobials tested appeared to provide additional data on their
antibacterial potency.
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