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Scientific
Publications - Work Done by Microbiology Reader
Scalarone, G.M., Mikami, Y., Kurita, N., Ichihara, Y., Yazawa, K. and Miyaji, M., Turbidometric characterization of the postantifungal effect: comparative studies with amphotericin B, 5-fluorocytosine and miconazole on Candida albicans, (Eng.), [Summary in Ger.: Turbidimetrische Charakterisierung des postantimyzetischen Effekts: Vergleichende Untersuchungen mit Amphotericin B, 5-Fluorcytosin und Miconazol and Candida albicans], Mycoses, 1991, vol. 34, pp. 297-302 ABSTRACT The phenomenon of persistent suppression of Candida albicans
yeast cell growth after short drug exposures (postantifungal effect, PAFE) was
determined by performing comparative studies using different concentrations of
5-fluorocytosine (5-FC), amphotericin B (AMPH) and miconazole (MCZ). An in vitro
turbidometric method was used to measure cell growth and to quantitate the PAFE
after removal of the drug by dilution following exposure to C. albicans yeast
cells for 0.5 h, 1 h or 2 h. The PAFE was determined by the difference in time
(h) required for growth of the control and test cultures to increase to the 0.5
absorbance level following removal of the antifungal agent. A PAFE was
demonstrated with each agent and generally the length of the PAFE was dependent
upon the concentration of the drug and the time of exposure. An exposure time of
0.5 h resulted in PAFEs ranging from 0.6 to 16.7 h with 5-FC, 0 to 16.5 h with
AMPH and 0.1 to 14.1 h with MCZ. In most instances exposure of the cells to each
drug for 1 or 2 h resulted in slightly longer PAFEs, respectively. Longer PAFEs
were induced with lower concentrations of 5-FC as compared to AMPH and MCZ. The
data from such PAFE assays may be useful for determining in vivo treatment
regimens, since longer PAFEs may allow for intermittent dosing instead of
continuous drug administration.
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