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Inheritance of Resistance to Bacillus thuringiensis subsp . kurstaki in Trichoplusia ni. Alida F. Janmaat, 2004.The genetic inheritance of resistance to a commercial formulation of Bacillus thuringiensis subsp . kurstaki was examined in a Trichoplusia ni colony initiated from a resistant population present in a commercial vegetable greenhouse in British Columbia, Canada . Progeny of F1 reciprocal crosses and backcrosses between F1 larvae and resistant (PR) and susceptible (PS) populations were assayed at different B . thuringiensis subsp . kurstaki concentrations . The responses of progeny of reciprocal F1 crosses were identical, indicating that the resistant trait was autosomal . The 50% lethal concentration for the F1 larvae was slightly higher than that for PS, suggesting that resistance is partially recessive . The responses of both backcross progeny (F1 x PR, F1 x PS) did not correspond to predictions from a single-locus model . The inclusion of a nonhomozygous resistant parental line in the monogenic model significantly increased the correspondence between the expected and observed results for the F1 x PR backcross but decreased the correspondence with the F1 x PS backcross results . This finding suggests that resistance to B . thuringiensis subsp . kurstaki in this T . ni population is due to more than one gene . Molecular Typing of Aeromonas Isolates in Natural Mineral Waters. P. Villari, 2003.A total of 103 isolates of Aeromonas spp . were obtained over a 3-year period from a natural mineral water and from surface streams located within the boundaries of the watershed of the natural mineral water wells and were typed by macrorestriction analysis of genomic DNA with XbaI and by pulsed-field gel electrophoresis . All Aeromonas caviae isolates from the natural mineral water belonged to the same clone, and an analogous clonal identity was found among Aeromonas hydrophila isolates . These two clones expressed no hemolytic or cytotoxic activities . Aeromonas isolates from surface waters showed high molecular heterogeneity and were not related to the clones found in the natural mineral water . The presence of aeromonads chronically found in the natural mineral water was a likely consequence of a localized development of a biofilm, with no exogenous contamination of the aquifer . Molecular fingerprinting of drinking water isolates is a useful tool in explaining possible reasons for bacterial occurrences . Receiver Operating Characteristic Curve Analysis of Beach Water Quality Indicator Variables. Ann Michelle Morrison, 2003.
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