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Scientific
Publications - Work Done by Microbiology Reader
Pastoni, F., Marengo, G. and Zampollo, F., Experimental Observations on the Presence of "Injured Cells" in Drinking Waters, Joint Research Centre, Environment Institute, Consumer Protection and Food Unit, Laboratory of Microbiology, 21020 Ispra, Italy, Report, 2000, 16 pp ABSTRACT Many chemical, physical and biological factors associated with
water have the potential to induce stress or injury in bacterial cells. Damaged
microorganisms, in particular, can be detected in water samples intended for
human consumption which have been treated with disinfectants. This investigation
was undertaken in order to collect experimental data on the presence of stressed
or injured microorganisms in mineral water samples collected from various EU
Countries which are not detectable using official methods of analysis. The study
has been carried out through the application of a recently developed analytical
procedure based on the study of bacteria and yeast growth kinetics. Analytical
determinations verified the presence of stressed or injured microorganisms in
reversible form in the examined water samples. The same samples frequently
showed no microbial growth when analyzed using traditional methods of analysis.
Therefore, in order to obtain a true indication of microbial numbers, the
samples must be subjected to some form of procedure for the recovery of injured
cells.
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