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Scientific
Publications - Work Done by Microbiology Reader
Mikami, Y., Scalarone, G.M., Kurita, N., Yazawa, K., Uno, J. and Miyaji, M., Synergistic postantifungal effect of flucytosine and fluconazole on Candida albicans, Journal of Medical and Veterinary Mycology, 1992, vol. 30, pp. 197-206 ABSTRACT The in vitro efficacy of flucytosine and fluconazole, separately
and in combination, with respect to induction of a postantifungal effect (PAFE)
on Candida albicans was studied. PAFE refers to the persistent suppression of
fungal cell growth following a short period of exposure to an antifungal agent.
A turbidometric method was used to measure cell growth and to quantitate the
PAFE following exposure of C. albicans yeast cells to different concentrations
of the two agents for 2 h. The PAFE was determined by the difference in time (h)
required for growth of the control and test cultures to increase to the 0.5
absorbance level following removal of the drug by dilution. Minimum (MIC) and
fractional inhibitory concentration determinations were made and the data used
for selecting the concentrations used in the PAFE evaluations. A synergistic
interaction of the two drugs at concentrations well below their individual MICs
was evidenced. Flucytosine:fluconazole ratios of 1:16-1:32 at concentrations
ranging from 0.024-0.098 micrograms ml-1 and from 0.78-1.56 micrograms ml-1,
with flucytosine and fluconazole, respectively, induced PAFEs which persisted
for 2.5 h longer than those achieved when each of the two agents was assayed
separately.
(order Full Text from publisher)
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