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Jose A. Simoes, Alla A. Aroutcheva, Susan Shott, Sebastian Faro, Effect
of metronidazole on the growth of vaginal lactobacilli in vitro,
Infectious Diseases in Obstetrics & Gynecology, Volume 9, Number 1, March
01, 2001, Pages: 41-45
ABSTRACT
Objective: To determine whether metronidazole has an adverse effect on
the growth of Lactobacillus.
Methods: Hydrogen peroxide- and bacteriocin-producing strains of
Lactobacillus were used as test strains. Concentrations of metronidazole
used ranged from 128 to 7000 wg/ml.
Susceptibility to metronidazole was conducted by the broth microdilution method
recommended by the National Committee for Clinical Laboratory Standards.
Results: Growth of Lactobacillus was partially inhibited at
concentrations between 1000 and 4000 wg/ml (p
= 0.014). Concentrations S 5000
wg/ml completely inhibited growth of
Lactobacillus. Concentrations between 128 and 256 wg/ml
stimulated growth of Lactobacillus (p = 0.025 and 0.005,
respectively). Concentrations of metronidazole between 64 and 128
wg/ml or S 512
wg/ml did not have an inhibitory or a
stimulatory effect on the growth of Lactobacillus compared to the control.
Conclusions: High concentration of metronidazole, i.e. between 1000 and
4000 wg/ml, partially inhibited the growth of
Lactobacillus. Concentrations S 5000
wg/ml completely suppressed the growth of
Lactobacillus. Concentrations between S 128
and h 256 wg/ml
stimulated the growth of Lactobacillus. Further investigation to
determine the ideal concentration of metronidazole is needed in order to use the
antimicrobial agent effectively in the treatment of bacterial vaginosis.
Keywords:
METRONIDAZOLE, LACTOBACILLUS, BACTERIAL VAGINOSIS
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