Scientific Publications - Work Done by Microbiology Reader Bioscreen C

Ji-Sook Han, Dong-Hwa Shin and Nam-In Baek, Identification of Growth Inhibitory Substance on Food-borne Microorganisms from Commiphora molmol Engl. and Its Application to Food Products, Korean Journal of Food Science and Technology, 2001, Vol. 33, No. 4, pp. 401-408

ABSTRACT

The ethanol extract and n-hexane fraction of Commiphora molmol Engl. showed minimum inhibitory concentration of 50 ppm and 25 ppm, respectively, on 5 strains of Listeria monocytogenes at 32ºC. The purified substance, C3-3-2 fraction, was isolated by silica gel column and preparative thin layer chromatography from n-hexane fraction of Commiphora molmol Engl. The C3-3-2 fraction showed a strong bactericidal activity on 5 strains of L. monocytogenes at the concentration of 10 ppm in tryptic soy broth medium. At that concentration, the viable count was reduced 5~6 log cycle from initial cell number. The n-hexane fraction of Commiphora molmol Engl. showed strong growth inhibition at the concentration of 25 ppm on Bacillus cereus and Staphylococcus aureus, at 50 ppm in broth on Salmonella enteritidis, and at 500 ppm on Vibrio parahaemolyticus. The purified antimicrobial substance, the C3-3-2 fraction, was identified as m-nonylphenol by on the basis of the ¹H-, ¹³C-NMR and EI/MS data. For the application test, the C3-3-2 fraction which was purely isolated from Commiphora molmol Engl. at 100 ppm were applied to minced Alaska pollack and ground beef at 32ºC and 5ºC. The antimicrobial substances did not reduce L. monocytogenes ATCC 19113 at 32ºC, while they reduced L. monocytogenes ATCC 19113 in viable number at 5ºC. However, the antimicrobial effect of C3-3-2 fraction in food system was lower than that of broth condition.

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