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Scientific Publications - Work Done by Microbiology Reader Bioscreen C

 

Aoyama, Isao, Application of DNA repair-deficient mutants of yeast Saccharomyces cerevisiae for genetic toxicity testing of chemicals, Nogaku Kenkyu (1991), 62(4), 271-81 [Journal written in Japanese]

ABSTRACT

The possibility of detecting DNA-damaging substances was investigated using DNA repair-deficient mutants of Saccharomyces cerevisiae. Yeast cells were incubated at 30° without shaking using a BIOSCREEN C system. Relative growth and survival rates were calcd. to analyze data. There are three kinds of independent DNA repair systems for yeast, excission repair, mutation repair, and recombination repair. The sensitivity to 4-nitroquinoline 1-oxide (4NQQ) obtained from EC50 values of the inhibition rate for relative growth rate is given. The mutant deficient in all DNA repair systems was the most sensitive in cell growth rate and the wild type was the least. 4NQQ damaged DNA and the intensity of the effect was dependent on the DNA repair sites.

 

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Last modified: May 25, 2005