Bio Docs

Multiplex Real-Time PCR Method To Identify Shiga Toxin Genes stx1 and stx2 and Escherichia coli O157:H7/H^- Serotype.
Karen C. Jinneman, 2003.A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E . coli O157:H7/H^- uidA gene has been developed . There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates . The PCR efficiencies were

1.89, and as few as 6 CFU/reaction could be detected .

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Last modified: May 25, 2005