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Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum ß-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay. Gerhard F. Weldhagen, 2004.The LightCycler was compared to nested PCR for the detection of blaGES/IBC genes from 100 Pseudomonas aeruginosa clinical isolates . The real-time PCR assay detected a blaGES/IBC gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing . The Streptomyces coelicolor Polynucleotide Phosphorylase Homologue, and Not the Putative Poly(A) Polymerase, Can Polyadenylate RNA. Björn Sohlberg, 2003.A protein containing a nucleotidyltransferase motif characteristic of poly(A) polymerases has been proposed to polyadenylate RNA in Streptomyces coelicolor (P . Bralley and G . H . Jones, Mol . Microbiol . 40:1155-1164, 2001) . We show that this protein lacks poly(A) polymerase activity and is instead a tRNA nucleotidyltransferase that repairs CCA ends of tRNAs . In contrast, a Streptomyces coelicolor polynucleotide phosphorylase homologue that exhibits polyadenylation activity may account for the poly(A) tails found in this organism .
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