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Proteomic Analysis of the Sarcosine-Insoluble Outer Membrane Fraction of Helicobacter pylori Strain 26695. Seung-Chul Baik, 2004.Helicobacter pylori causes gastroduodenal disease, which is mediated in part by its outer membrane proteins [OMPs] . To identify OMPs of H . pylori strain 26695, we performed a proteomic analysis. A sarcosine-insoluble outer membrane fraction was resolved by two-dimensional electrophoresis with immobilized pH gradientstrips . Most of the protein spots, with molecular masses of10 to 100 kDa, were visible on the gel in the alkaline pI regions[6.0 to 10.0] . The proteome of the OMPs was analyzed by matrix-assistedlaser desorption ionization-time-of-flight mass spectrometry.Of the 80 protein spots processed, 62 spots were identified;they represented 35 genes, including 16 kinds of OMP . Moreover,we identified 9 immunoreactive proteins by immunoblot analysis.This study contributes to the characterization of the H . pyloristrain 26695 proteome and may help to further elucidate thebiological function of H . pylori OMPs and the pathogenesis ofH . pylori infection. Alterations of Penicillin-Binding Proteins 1A, 2X, and 2B in Streptococcus pneumoniae Isolates for Which Amoxicillin MICs Are Higher than Penicillin MICs. K. Kosowska, 2004.Penicillin-binding proteins (PBPs) of 15 selected penicillin- and amoxicillin-resistant Streptococcus pneumoniae isolates (MICs of 2 to 8 and 8 to 16 µg/ml, respectively) were studied . In addition to typical changes in PBPs 1A and 2X, these strains had 10 unique changes in PBP 2B, including a 618A-G substitution, which may be the key alteration associated with amoxicillin resistance . Effect of Ertapenem Protein Binding on Killing of Bacteria. David E. Nix, 2004.The effect of protein binding on the antimicrobial activity of ertapenem was evaluated using the bacterial kill rate and concentration-response studies . Various proportions of human serum were utilized to determine the total and free-drug concentrations using a validated high-performance liquid chromatography assay . The MICs and kill curves were determined for test isolates of Enterobacter cloacae and Staphylococcus aureus at various percentages of human serum . The killing of bacteria was analyzed in relation to the free and total concentrations of ertapenem at various proportions of human serum . It was determined that unbound ertapenem was responsible for the antimicrobial activity against the test isolates . Repeated Introduction of Genetically Modified Pseudomonas putida WCS358r without Intensified Effects on the Indigenous Microflora of Field-Grown Wheat. M. Viebahn, 2003.
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