|
|
|
|
|
|
Determination of Susceptibilities of 26 Leptospira sp . Serovars to 24 Antimicrobial Agents by a Broth Microdilution Technique. Clinton K. Murray, 2004.The MICs of 24 antimicrobials for 26 Leptospira spp . serovars were determined using a broth microdilution technique . The MICs at which 90% of isolates tested were inhibited (MIC90s) of cefepime, imipenem-cilastatin, erythromycin, clarithromycin, and telithromycin were all  0.01 µg/ml . The MIC90s of amoxicillin, aztreonam, cefdinir, chloramphenicol, and penicillin G were
 3.13 µg/ml . Many antimicrobials have excellent in vitro activity against Leptospira .
Comparative Analysis of the Escherichia coli Ketopantoate Hydroxymethyltransferase Crystal Structure Confirms that It Is a Member of the (ß  )8 Phosphoenolpyruvate/Pyruvate Superfamily.Florian Schmitzberger, 2003.Escherichia coli ketopantoate hydroxymethyltransferase (KPHMT) catalyzes the first step in the biosynthesis pathway of pantothenate (vitamin B5), the transfer of a hydroxymethyl group onto -ketoisovalerate . Here we describe a detailed comparative analysis of the KPHMT crystal structure and the identification of structural homologues, some of which have remarkable similarities in their active sites, modes of binding to substrates, and mechanisms . We show that KPHMT forms a family within the phosphoenolpyruvate/pyruvate superfamily . Based on the analysis, we propose that in this superfamily there should be a subdivision into two groups . This paper completes our structural analysis of the E . coli enzymes in the pantothenate pathway .
Molecular Identification of Ectomycorrhizal Mycelium in Soil Horizons. Renske Landeweert, 2003.Molecular identification techniques based on total DNA extraction provide a unique tool for identification of mycelium in soil . Using molecular identification techniques, the ectomycorrhizal (EM) fungal community under coniferous vegetation was analyzed . Soil samples were taken at different depths from four horizons of a podzol profile . A basidiomycete-specific primer pair (ITS1F-ITS4B) was used to amplify fungal internal transcribed spacer (ITS) sequences from total DNA extracts of the soil horizons . Amplified basidiomycete DNA was cloned and sequenced, and a selection of the obtained clones was analyzed phylogenetically . Based on sequence similarity, the fungal clone sequences were sorted into 25 different fungal groups, or operational taxonomic units (OTUs) . Out of 25 basidiomycete OTUs, 7 OTUs showed high nucleotide homology (  99%) with known EM fungal sequences and 16 were found exclusively in the mineral soil . The taxonomic positions of six OTUs remained unclear . OTU sequences were compared to sequences from morphotyped EM root tips collected from the same sites . Of the 25 OTUs, 10 OTUs had
98% sequence similarity with these EM root tip sequences . The present study demonstrates the use of molecular techniques to identify EM hyphae in various soil types . This approach differs from the conventional method of EM root tip identification and provides a novel approach to examine EM fungal communities in soil .
|
© 2005
Transgalactic Ltd (manufacturer of Bioscreen C software) |
Privacy Statement | P.O. Box
1393, 00101 Helsinki, Finland,
Last modified: May 25, 2005
| ||||||
