Textbook

Development of a Multispecies Oral Bacterial Community in a Saliva-Conditioned Flow Cell.
Jamie S. Foster, 2004.Microbial communities within the human oral cavity are dynamic associations of more than 500 bacterial species that form biofilms on the soft and hard tissues of the mouth . Understanding the development and spatial organization of oral biofilms has been facilitated by the use of in vitro models . We used a saliva-conditioned flow cell, with saliva as the sole nutritional source, as a model to examine the development of multispecies biofilm communities from an inoculum containing the coaggregation partners Streptococcus gordonii, Actinomyces naeslundii, Veillonella atypica, and Fusobacterium nucleatum . Biofilms inoculated with individual species in a sequential order were compared with biofilms inoculated with coaggregates of the four species . Our results indicated that flow cells inoculated sequentially produced biofilms with larger biovolumes compared to those biofilms inoculated with coaggregates . Individual-species biovolumes within the four-species communities also differed between the two modes of inoculation . Fluorescence in situ hybridization with genus- and species-specific probes revealed that the majority of cells in both sequentially and coaggregate-inoculated biofilms were S . gordonii, regardless of the inoculation order . However, the representation of A . naeslundii and V . atypica was significantly higher in biofilms inoculated with coaggregates compared to sequentially inoculated biofilms . Thus, these results indicate that the development of multispecies biofilm communities is influenced by coaggregations preformed in planktonic phase . Coaggregating bacteria such as certain streptococci are especially adapted to primary colonization of saliva-conditioned surfaces independent of the mode of inoculation and order of addition in the multispecies inoculum . Preformed coaggregations favor other bacterial strains and may facilitate symbiotic relationships .

What Is Anthrax?, What Is Botulism?, What is Food Microbiology?, What Is Molecular Microbiology?, What Is Biofilm?, a, Bacteriology, e, Microbe, a, Microorganism, s, Bacteria, e, Microbiology, c, Haemophilus, o, Haemophilus, n, Micrococci, s, Escherichia coli, r, Antibiotics, o, Bacillus subtilis, c, S. cerevisiae

Scientific Publications - Work Done by Microbiology Reader Bioscreen C
Agricultural Microbiology Anaerobic Microbiology Antimicrobial Susceptibility Artificial Atmosphere Bioassay of Antibiotics Biofilm Microbiology Bioreactor Technology Biotechnology Cell Biology Clinical Microbiology Environmental Microbiology Experiments with Yeast	Fermentation Food Microbiology Functional Genomics Gene Technology Growth Media Development Growth Rate and Lag Time Industrial Microbiology Medical/Pharmaceutical Field Microbiological Assay Microbiological Research Microbiology of Cosmetics go to a specific theme...	Military Microbiology Molecular Microbiology Mutagenicity and Genotoxicity Oral Microbiology Patents Postantibiotic Studies Soil Microbiology Spore Microbiology Veterinary Microbiology Waste/Wastewater Treatment Water Microbiology Wine Microbiology

© 2005 Transgalactic Ltd (manufacturer of Bioscreen C software) | Privacy Statement | P.O. Box 1393, 00101 Helsinki, Finland, phone: +358 9 85172920, fax: +358 9 8749481, e-mail: microbiology@bionewsonline.com

Last modified: May 25, 2005