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Factors and Global Gene Regulation in Mycobacterium tuberculosis. Riccardo Manganelli, 2004. Learning from Nature's Drug Factories: Nonribosomal Synthesis of Macrocyclic Peptides. Stephan A. Sieber, 2003. Purification and Characterization of a Prolyl Aminopeptidase from Debaryomyces hansenii. Tomás Bolumar, 2003.A prolyl aminopeptidase (PAP) (EC 3.4.11.5) was isolated from the cell extract of Debaryomyces hansenii CECT12487 . The enzyme was purified by selective fractionation with protamine and ammonium sulfate, followed by two chromatography steps, which included gel filtration and anion-exchange chromatography . The PAP was purified 248-fold, with a recovery yield of 1.4% . The enzyme was active in a broad pH range (from 5 to 9.5), with pH and temperature optima at 7.5 and 45°C . The molecular mass was estimated to be around 370 kDa . The presence of inhibitors of serine and aspartic proteases, bestatin, puromycin, reducing agents, chelating agents, and different cations did not have any effect on the enzyme activity . Only iodoacetate, p-chloromercuribenzoic acid, and Hg2+, which are inhibitors of cysteine proteases, markedly reduced the enzyme activity . The Km for proline-7-amido-4-methylcoumarin was 40 µM . The enzyme exclusively hydrolyzed N-terminal-proline-containing substrates . This is the first report on the identification and purification of this type of aminopeptidase in yeast, which may contribute to the scarce knowledge about D . hansenii proteases and their possible roles in meat fermentation . Endotoxin Inactivation in Water by Using Medium-Pressure UV Lamps. W. B. Anderson, 2003.Deionized water was spiked with various concentrations of endotoxin and exposed to UV irradiation from medium-pressure UV lamps to assess endotoxin inactivation . It was found that endotoxin inactivation was proportional to the UV dose under the conditions examined . The inactivation rate was determined to be  0.55 endotoxin unit/ml per mJ/cm2 of irradiation delivered .
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