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Susceptibility of Chlamydia trachomatis to Excipients Commonly Used in Topical Microbicide Formulations. M. F. Lampe, 2004.Commonly used "inactive" pharmaceutical excipients were tested in a previously developed minimum cidal concentration assay to assess their ability to kill Chlamydia trachomatis topically . Sixteen excipients were evaluated in these studies under various conditions . A range of activities was found among the excipients that could be tested in our assay system . Cyanobacterial Ecotypes in Different Optical Microenvironments of a 68°C Hot Spring Mat Community Revealed by 16S-23S rRNA Internal Transcribed Spacer Region Variation. Mike J. Ferris, 2003.We examined the population of unicellular cyanobacteria (Synechococcus) in the upper 3-mm vertical interval of a 68°C region of a microbial mat in a hot spring effluent channel (Yellowstone National Park, Wyoming) . Fluorescence microscopy and microsensor measurements of O2 and oxygenic photosynthesis demonstrated the existence of physiologically distinct Synechococcus populations at different depths along a light gradient quantified by scalar irradiance microprobes . Molecular methods were used to evaluate whether physiologically distinct populations could be correlated with genetically distinct populations over the vertical interval . We were unable to identify patterns in genetic variation in Synechococcus 16S rRNA sequences that correlate with different vertically distributed populations . However, patterns of variation at the internal transcribed spacer locus separating 16S and 23S rRNA genes suggested the existence of closely related but genetically distinct populations corresponding to different functional populations occurring at different depths . Precipitation of Barite by Myxococcus xanthus: Possible Implications for the Biogeochemical Cycle of Barium. Maria Teresa González-Muñoz, 2003.Bacterial precipitation of barite (BaSO4) under laboratory conditions is reported for the first time . The bacterium Myxococcus xanthus was cultivated in a solid medium with a diluted solution of barium chloride . Crystallization occurred as a result of the presence of live bacteria and the bacterial metabolic activity . A phosphorous-rich amorphous phase preceded the more crystalline barite formation . These experiments may indicate the involvement of bacteria in the barium biogeochemical cycle, which is closely related to the carbon cycle .
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