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Biofilm Mode of Growth of Streptococcus intermedius Favored by a Competence-Stimulating Signaling Peptide. Fernanda C. Petersen, 2004.Gram-positive and gram-negative bacteria use quorum sensingto coordinate population behavior . In several streptococci,quorum sensing mediated by competence-stimulating peptides [CSP]is associated with development of competence for transformation.We show here that a synthetic CSP favored the biofilm mode ofgrowth of Streptococcus intermedius without affecting the rateof culture growth. Toward an International Standard for PCR-Based Detection of Food-Borne Thermotolerant Campylobacters: Validation in a Multicenter Collaborative Trial. P. S. Lübeck, 2003.As part of a European research project, the performance of a PCR assay to detect food-borne thermotolerant campylobacters (Campylobacter jejuni, C . coli, and C . lari) was evaluated through an international collaborative trial involving 12 participating laboratories . DNA from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis . The overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) was 100% . The results indicate that the assay can become an international standard and can be confidently applied in microbiological laboratories .
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